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Characterization of high fluoride resistant Pseudomonas aeruginosa species isolated from water samples

Year 2022, Volume: 5 Issue: 4, 325 - 339, 31.12.2022
https://doi.org/10.35208/ert.1070624

Abstract

Groundwater fluoride contamination is one of the most serious toxicological environmental issues in India and around the world. Water samples were taken from Natham taluk and screened for fluoride resistant bacteria. Initially, twenty-four fluoride resistant colonies were selected from 50 mM NaF supplemented LB agar plates. On blood agar plates, all isolates showed β-haemolysis, confirming their status as pathogens. Virulence factors (algD, plcH, toxA, gyrB, rhlC, lasB) and biofilm-forming genes (ppyR, pelA, pslA) were identified in these isolates by PCR analysis. The fluoride ion transporter ‘crcB’ was successfully amplified from these isolates by gene-specific PCR. Genus Pseudomonas and P. aeruginosa species-specific PCR analysis identified that all strains as belonging to the P. aeruginosa species. Besides, three high fluoride resistance strains were selected based on high fluoride resistance and confirmed as P. aeruginosa species by 16S rRNA sequencing and NCBI blast analysis.

Supporting Institution

This work was supported by grant from Science and Engineering Research Board (SERB), DST, New Delhi, India, Ramanujan Fellowship to C. Edward Raja .

Project Number

Award No:SB/S2/RJN-009/2016

References

  • [1] W.M. Edmunds and P.L. Smedley, Fluoride in natural waters, Essentials of medical geology, [O. Selinus (eds.)]. Springer, Netherlands, pp. 311-336, 2001
Year 2022, Volume: 5 Issue: 4, 325 - 339, 31.12.2022
https://doi.org/10.35208/ert.1070624

Abstract

Project Number

Award No:SB/S2/RJN-009/2016

References

  • [1] W.M. Edmunds and P.L. Smedley, Fluoride in natural waters, Essentials of medical geology, [O. Selinus (eds.)]. Springer, Netherlands, pp. 311-336, 2001
There are 1 citations in total.

Details

Primary Language English
Subjects Environmental Sciences
Journal Section Research Articles
Authors

Chellaiah Edward Raja 0000-0002-5559-7946

Ravi Pandeeswari This is me 0000-0003-4829-9534

U Ramesh This is me 0000-0002-5100-2191

Project Number Award No:SB/S2/RJN-009/2016
Publication Date December 31, 2022
Submission Date February 10, 2022
Acceptance Date November 2, 2022
Published in Issue Year 2022 Volume: 5 Issue: 4

Cite

APA Edward Raja, C., Pandeeswari, R., & Ramesh, U. (2022). Characterization of high fluoride resistant Pseudomonas aeruginosa species isolated from water samples. Environmental Research and Technology, 5(4), 325-339. https://doi.org/10.35208/ert.1070624
AMA Edward Raja C, Pandeeswari R, Ramesh U. Characterization of high fluoride resistant Pseudomonas aeruginosa species isolated from water samples. ERT. December 2022;5(4):325-339. doi:10.35208/ert.1070624
Chicago Edward Raja, Chellaiah, Ravi Pandeeswari, and U Ramesh. “Characterization of High Fluoride Resistant Pseudomonas Aeruginosa Species Isolated from Water Samples”. Environmental Research and Technology 5, no. 4 (December 2022): 325-39. https://doi.org/10.35208/ert.1070624.
EndNote Edward Raja C, Pandeeswari R, Ramesh U (December 1, 2022) Characterization of high fluoride resistant Pseudomonas aeruginosa species isolated from water samples. Environmental Research and Technology 5 4 325–339.
IEEE C. Edward Raja, R. Pandeeswari, and U. Ramesh, “Characterization of high fluoride resistant Pseudomonas aeruginosa species isolated from water samples”, ERT, vol. 5, no. 4, pp. 325–339, 2022, doi: 10.35208/ert.1070624.
ISNAD Edward Raja, Chellaiah et al. “Characterization of High Fluoride Resistant Pseudomonas Aeruginosa Species Isolated from Water Samples”. Environmental Research and Technology 5/4 (December 2022), 325-339. https://doi.org/10.35208/ert.1070624.
JAMA Edward Raja C, Pandeeswari R, Ramesh U. Characterization of high fluoride resistant Pseudomonas aeruginosa species isolated from water samples. ERT. 2022;5:325–339.
MLA Edward Raja, Chellaiah et al. “Characterization of High Fluoride Resistant Pseudomonas Aeruginosa Species Isolated from Water Samples”. Environmental Research and Technology, vol. 5, no. 4, 2022, pp. 325-39, doi:10.35208/ert.1070624.
Vancouver Edward Raja C, Pandeeswari R, Ramesh U. Characterization of high fluoride resistant Pseudomonas aeruginosa species isolated from water samples. ERT. 2022;5(4):325-39.