Evaluation of Antimicrobial Effects of Some Plant Species Growing in Turkiye: Verbascum lydium Boiss. var. lydium Boiss., Euphorbia anacampseros Boiss. var. tmolea M.S. Khan., Rosa pisiformis subsp. pisiformis (Christ) D. Sosn., Stachys tmolea Boiss. subsp. tmolea and Aronia melanocarpa (Michx.) Elliott

Year 2024, Volume: 17 Issue: 1, 91 - 99, 28.03.2024

Çiğdem Bilgi , Hatice Demiray , Engin Kaplan

https://doi.org/10.18185/erzifbed.1329880

Abstract

Abstract
The increasing prevalence of antibiotic-resistant bacteria has emerged as a critical global public health issue, necessitating the exploration of alternative measures to combat infectious diseases. Traditional medicinal plants have been long recognized for their potential in providing natural compounds with antimicrobial properties for various therapeutic purposes. The diverse plant biodiversity in Turkey offers a promising potential for the discovery of novel antimicrobial compounds. Therefore, this study aimed to investigate the antimicrobial activities of methanolic extracts from several endemic and non-endemic plant species against various microorganisms. The endemic species included Verbascum lydium Boiss. var. lydium (stem), Euphorbia anacampseros var. tmolea Boiss. (root), Stachys tmolea subsp. tmolea Boiss. (leaves and stem), Rosa pisiformis subsp. pisiformis (fruit, petiole, root, leaves), while non-endemic species were represented by cultivated Aronia melanocarpa (fruit). In this research, the effectiveness of V. lydium, E. anacampseras, R. pisiformis (all tested parts) and A. melanocarpa (fruits) were demonstrated against Staphylococcus aureus and Escherichia coli. Methanolic extracts of the roots and leaves of R. pisiformis showed the most promising bioactivity with a MIC value of 62.5 µg mL-1 on Pseudomonas aeruginosa among all tested extracts. In addition, the results showed that antimicrobial bioactivities were observed for Candida parapsilosis, C. glabrata and Klebsiella pneumoniae for V. lydium; S. tmolea and R. pisiformis with MIC values of 31.5 µg mL-1 per each. The findings of this study could provide valuable information for future research into the use of Turkish plants for pharmacological purposes, public health and traditional medicine applications.

Keywords

Verbascum lydium, Euphorbia anacampseras, Rosa pisiformis, Aronia melanocarpa, Stachys tmolea, antimicrobial.

Project Number

-

References

• [1].Khan, Rosina, Barira Islam, Mohd Akram, Shazi Shakil, Anis Ahmad, S Manazir Ali, Mashiatullah Siddiqui, and Asad U Khan. 2009. “Antimicrobial Activity of Five Herbal Extracts against Multi Drug Resistant (MDR) Strains of Bacteria and Fungus of Clinical Origin.” Molecules 14 (2): 586–97.
• [2].Cragg, Gordon M, and David J Newman. 2013. “Natural Products: A Continuing Source of Novel Drug Leads.” Biochimica et Biophysica Acta (BBA)-General Subjects 1830 (6): 3670–95.
• [3].Mahady, Gail B. 2005. “Medicinal Plants for the Prevention and Treatment of Bacterial Infections.” Current Pharmaceutical Design 11 (19): 2405–27.
• [4].Arif, Tasleem, J D Bhosale, Naresh Kumar, T K Mandal, R S Bendre, G S Lavekar, and Rajesh Dabur. 2009. “Natural Products–Antifungal Agents Derived from Plants.” Journal of Asian Natural Products Research 11 (7): 621–38.
• [5]Shakeri, A R, and A Farokh. 2015. “Phytochemical Evaluation and Antioxidant Activity of Verbascum Sublobatum Murb. Leaves.” Research Journal of Pharmacognosy 2 (3): 43–47.
• [6].Panchal, Mayank A, Krishna Murti, and Vijay Lambole. 2010. “Pharmacological Properties of Verbascum Thapsus—A Review.” Int J Pharm Sci Rev Res 5 (2): 73–77.
• [7].Jamshidi-Kia, Fatemeh, Zahra Lorigooini, Sedigheh Asgari, and Karamatollah Saeidi. 2018. “Iranian Species of Verbascum: A Review of Botany, Phytochemistry, and Pharmacological Effects.” Toxin Reviews.
• [8].Tatli, I Irem, and Zeliha Fi Akdemir. 2006. “Traditional Uses and Biological Activities of Verbascum Species.” FABAD Journal of Pharmaceutical Sciences 31 (2): 85.
• [9].Ertuğ, Füsun. 2004. “Wild Edible Plants of the Bodrum Area (Muğla, Turkey).” Turkish Journal of Botany 28 (1): 161–74.
• [10].Gokcen, Ümmüşen, Onur Koyunvu, and Okan Sezer. 2018. “Euphorbia Anacampseros Boiss. Var. Anacampseros Üzerinde Morfo-Anatomik Araştırmalar.” Biyoloji Bilimleri Araştırma Dergisi 11 (2): 11–13.
• [11]Tutgun, Gizem, and Ahmet Gönüz. 2020. “An Endemic Plant Growing In Kazdaği Importance And Usage Areas Of Euphorbia Anacampseros Boiss. Var. Anacampseros taxon.” Journal of Scientific Perspectives 4 (4): 281–88.
• [12].Askun, Tülin, Emmanuel Mouafo Tekwu, Fatih Satil, Seyma Modanlioglu, and Hatice Aydeniz. 2013. “Preliminary Antimycobacterial Study on Selected Turkish Plants (Lamiaceae) against Mycobacterium Tuberculosis and Search for Some Phenolic Constituents.” BMC Complementary and Alternative Medicine 13 (1): 1–11.
• [13].Demiray, Hatice, Nurhayat Tabanca, Alden S Estep, James J Becnel, and Betül Demirci. 2019. “Chemical Composition of the Essential Oil and N-Hexane Extract of Stachys Tmolea Subsp. Tmolea Boiss., an Endemic Species of Turkey, and Their Mosquitocidal Activity against Dengue Vector Aesdes Aegypti.” Saudi Pharmaceutical Journal 27 (6): 877–81.
• [14].Elfalleh, Walid, Bulent Kirkan, and Cengiz Sarikurkcu. 2019. “Antioxidant Potential and Phenolic Composition of Extracts from Stachys Tmolea: An Endemic Plant from Turkey.” Industrial Crops and Products 127: 212–16.
• [15].Leblebici, Sema, Özge Kaygusuz, Tülin Korkmaz, and Cihan Darcan. 2016. “The Antimicrobial Activities of the Leaves of Some Endemic Stachys Species Spreading in West Anatolia, Turkey.” Mitteilungen Klosterneuburg.
• [16].Selahvarzian, Amin, Abuzar Alizadeh, Peyman Amanolahi Baharvand, Omayma A Eldahshan, and Bahram Rasoulian. 2018. “Medicinal Properties of Rosa Canina L.” Herbal Medicines Journal (Herb Med J), 77–84.
• [17].Dalar, Abdullah, Muzaffer Mukemre, Murat Unal, and Fevzi Ozgokce. 2018. “Traditional Medicinal Plants of Ağrı Province, Turkey.” Journal of Ethnopharmacology 226: 56–72.
• [18].Ercisli, S, E Orhan, and A Esitken. 2007. “Fatty Acid Composition of Rosa Species Seeds in Turkey.” Chemistry of Natural Compounds 43: 605–6.
• [19].Ercisli, Sezai. 2007. “Chemical Composition of Fruits in Some Rose (Rosa Spp.) Species.” Food Chemistry 104 (4): 1379–84.
• [20].Yilmaz, Suzan Ozturk, and SEZAI Ercisli. 2011. “Antibacterial and Antioxidant Activity of Fruits of Some Rose Species from Turkey.” Romanian Biotechnological Letters 16 (4): 6407–11
• [21]. Institute, Clinical and Laboratory Standards. 2006. “Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically.” Approved Standard M7-A7. Clinical and Laboratory Standards Institute Wayne, PA.
• [22].Wayne, P A. 2002. “Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts, Approved Standard.” CLSI Document M27-A2.
• [23].Zhang, X., Song, M., Xu, W., & Xu, W. (2023). Polyphenols extracted from Aronia melanocarpa by combined enzymatic and ultrasonication and their antioxidant potential and antimicrobial activity. 多酚, 5(1), 35-47.
• [24].Tanağardı, D. (2023). Antimicrobial, antioxidant, antiproliferative and cytotoxic activities of aronia fruit extract (Master's thesis, 01. Izmir Institute of Technology).