EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA

Year 2020, Volume: 8 Issue: 2, 293 - 302, 31.08.2020

Muhammet Ceylan Shang-you Yang Ramazan Asmatulu

https://doi.org/10.20290/estubtdb.658998

Abstract

Polycaprolactone (PCL) nanofiber with constant plasmid DNA addition and with different concentration of gentamicin designed and studied for their effect on cell viability and release rate. PCL nanofibers were fabricated using electrospinning method with plasmid DNA and gentamicin addition. The plasmid DNA used in PCL nanofiber were extracted from E. coli. The scanning electron microscopy (SEM) images show that the nano-scale fiber structures have an average diameter of 113.9 nm. UV microplate reader confirmed the existence of plasmid DNA in the PCL nanofibers. Elisa reader study showed the addition of gentamicin in the fibers. Cell viability tests indicated that PCL nanofibers with 10% gentamicin on a fibroblast cell showed high cell viability, which is related to surface areas and pore size of the electrospun fibers besides to the interaction among gentamicin, plasmid DNA and electrospun fiber matrix.

Keywords

Electrospinning, PCL nanofibers, Wound Dressing, Cell viability

GENTAMICIN YÜKLENEN PCL NANOFİBERLERİNİN ÇEŞİTLİLİK HÜCRESİNE ETKİSİ VE PLAZMİD DNA ORANI

Abstract

Sabit plazmid DNA ilaveli ve farklı gentamisin konsantrasyonlu polikaprolakton (PCL) nano elyafı, hücre canlılığı ve salıverme hızı üzerindeki etkileri için tasarlanmış ve çalışılmıştır. PCL nano lifleri, plazmid DNA ve gentamisin ilavesiyle elektrospinning yöntemi kullanılarak imal edildi. PCL nanofiberinde kullanılan plazmid DNA, E. coli'den özümlendi. Taramalı elektron mikroskobu (SEM) görüntüleri nano ölçekli elyaf yapılarının ortalama 113.9 nm çapa sahip olduğunu göstermektedir. UV mikroplaka okuyucu, PCL nano fiberlerinde plazmid DNA'nın varlığını doğruladı. Elisa okuyucu çalışması liflere gentamisin eklendiğini gösterdi. Hücre canlılığı testleri, bir fibroblast hücresi üzerinde% 10 gentamisin içeren PCL nano elyaflarının, gentamisin, plazmid DNA ve elektrospun elyaf matrisi arasındaki etkileşimin yanı sıra, elektrospun liflerinin yüzey alanları ve gözenek büyüklüğü ile ilgili yüksek hücre canlılığı gösterdiğini göstermiştir.

Keywords

Elektrospinleme, PCL nano lifleri, Hücre canlılığı, Yara Pansuman

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