Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 2667-4203 Eskisehir Technical University 10.18036/estubtdc.583152 Microbiology Mikrobiyoloji KANSER İMMÜNOTERAPİSİNDE KULLANILMAK ÜZERE PHBHHX BAZLI KATI LİPİD NANOPARTİKÜL DİZAYNI https://orcid.org/0000-0003-3176-0902 Laçin Türkoğlu Nelisa YILDIZ TEKNİK ÜNİVERSİTESİ 07 30 2020 9 2 239 248 06 27 2019 07 10 2020 Copyright © 2010, 2010

Kanser tedavisinde immünoterapi yaklaşımının kullanılması her geçen gün popüleritisini arttırarak bu alanda yerini sağlamlaştırmaktadır. Mevcut kanser immünoterapi sistemleri ile yapılan tedaviler tek başına istenen verimlilikte çalışmamaktadır. İlgili alanda terapötik maddelerin tümöre iletilmesi için oldukça karmaşık ve sınırlayıcı olan tümör mikroçevresini aşarak tümör hücresine iletilmesi gerekmektedir. Bu çalışmada, IL-6 kodlayan plazmid DNA model olarak kullanılarak, plazmidi tümör hücrelerine aktarabilecek bir taşıyıcı sisteminin geliştirilmesi üzerine odaklanılmıştır. Farklı miktarlarda bileşenler kullanılarak ve farklı koşullarda çözücü difüzyon yöntemiyle bir katı lipit katyonik nanopartikül sisteminin üretimi hedeflenmiştir. Elde edilen nanotaşıyıcının kimyasal yapısı ATR-FTIR spektroskopisi ile karakterize edilmiştir. Zeta-sizer cihazı kullanılarak, nanopartiküllerin ortalama büyüklüğünün 208,1 nm (± 256,8) [Polidispersite Endeksi (PDI), 0,06] ve 13,7 mV (± 5,4) zeta potansiyeline sahip olduğu tespit edilmiştir. Nanopartiküllerin morfolojik karakterizasyonu taramalı elektron mikroskobu (SEM), atomik kuvvet mikroskobu (AFM) cihazları ile incelenmiştir. PHBHHX bazlı SLN'nin sitotoksisitesi, fare fibroblast hücre hattında (L929) MTT testi ile belirlenmiştir.PHBHHX bazlı nanopartikül, yeşil floresan protein (GFP) ve IL-6 kodlayan plazmid DNA ile konjuge edildikten sonra MCF-7 hücrelerine transfeksiyonu gerçekleştirilip, transfeksiyon verimi GFP ekspresyonunu floresan mikroskobu ile takip edilmesi ile belirlenmiştir. GFP ekspresyon etkinliği %73 (± 5,8) olarak belirlenmiştir.

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