ejm

Ege Tıp Dergisi

1016-9113 2147-6500

Ege University

10.19161/etd.1704479

Obstetrics and Gynaecology

Kadın Hastalıkları ve Doğum

Deneysel prematüre yumurtalık yetmezliği modelinde kullanılan antioksidanların mitofaji proteinleri ve bunlarla ilişkili MikroRNA'lar üzerindeki etkileri

Effects of antioxidants used in the experimental premature ovarian insufficiency model on mitophagy proteins and them-associated MicroRNAs

https://orcid.org/0000-0003-4751-5944

Uçar Kartal

Duygu

Manisa Merkezefendi Devlet Hastanesi

https://orcid.org/0000-0002-7953-9361

Kuşçu

Naci Kemal

MANİSA CELÂL BAYAR ÜNİVERSİTESİ

https://orcid.org/0000-0001-6627-5443

Özbilgin

Kemal

MANISA CELAL BAYAR UNIVERSITY

https://orcid.org/0000-0003-1040-0393

Pekmezci

Muhammed Yusuf

MANISA CELAL BAYAR UNIVERSITY

12 08 2025

64 4 688 700 05 22 2025 08 28 2025

1962

Ege Journal of Medicine

Amaç: Deneysel olarak Prematür Ovaryen Yetmezlik (POI) oluşturulmuş sıçanlar üzerinde; quercetin ve curcumin ile parkin bağımlı ve bağımsız mekanizmalarda meydana gelen değişiklikler inceledi. Folikül sayısı, immünohistokimyasal proteinler, mRNA'lar ve bunlarla ilişkili mikroRNA'ları inceleyerek POI'de quercetin ve curcuminin koruyucu ve tedavi edici etkilerini araştırmak amaçlandı.Gereç ve Yöntem: Çalışmamızda sıçanlarda siklofosfamid aracılığıyla deneysel prematüre over yetmezliği modeli oluşturuldu. Daha sonra dokular histopatolojik yöntemler ve histokimyasal boyama ile incelendi. Over örneklerinde RNA izolasyonu yapıldı ve cDNA sentezi ve qPCR işlemleri gerçekleştirildi.Bulgular: Elde edilen bulgular ile gruplar arasındaki fark istatistiksel olarak değerlendirildi.

Aim: It was aimed to investigate the protective and therapeutic effects of quercetin and curcumin in POI by examining follicle count, immunohistochemical proteins, mRNAs and their related microRNAs.Materials and Methods: In our study, an experimental premature ovarian insufficiency model was created on rats by means of cyclophosphamide. Then the tissues were examined by histopathological methods and histochemical staining. We studied the changes in parkin-dependent and independent mechanisms that occur with quercetin and curcumin. RNA isolation was performed on ovarian samples and cDNA synthesis, and qPCR processes were performed. The difference between the obtained findings and the groups was evaluated statistically.Results: Cyclophosphamide depleted primordial follicles, which is the main pool for fertility, on ovarian tissue and induced atresia (p

Cyclophosphamide Curcumin Quercetin MiRNA POI Parkin FUNDC1

Siklofosfamid Kurkumin Kuersetin MiRNA POI Parkin FUNDC1.

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