Biyo-Primingin İtalyan Çimi (Lolium multiflorum Lam.) Tohum Çimlenmesi ve Fide Büyümesi Üzerine Etkileri

Year 2024, Volume: 7 Issue: 2, 111 - 114

Onur Okumuş , Büşra Gün , Semih Yılmaz , Satı Uzun

https://doi.org/10.55257/ethabd.1559267

Abstract

Bitki büyümesini teşvik eden rizobakteriler (PGPR) ile tohum biyo-priming uygulamaları, çimlenmeyi ve fide gelişimini iyileştirmek amacıyla son zamanlarda yaygın olarak kullanılmaktadır. Bu nedenle, bu çalışmanın amacı, farklı bakteri suşları ile biyo-priming uygulamalarının İtalyan çimi tohumlarının çimlenmesi ve fide gelişimi üzerindeki etkilerini araştırmaktır. Çalışmada Elif çeşidi (Lolium multiflorum Lam) İtalyan çimi tohumları kullanılmıştır ilk olarak sterilize edilmiş tohumlar Bacillus türlerine ait dokuz farklı bakteri suşu (108 cfu/mL bakteri süspansiyonu) ile 120 rpm'de 15 dakika süreyle inoküle edilmiş ve ardından oda sıcaklığında kurutulmuştur. Uygulama yapılan tohumlar petri kaplarında 3 filtre kağıdı arasında 25 tohum olacak şekilde 22 ±2 ˚C'de çimlendirilmiştir. Çalışma üç tekerrürlü olarak tesadüf parselleri deneme desenine göre yürütülmüştür. Çalışma sonucunda, çimlenme yüzdesi ve kök uzunluğu bakımından uygulamalar arasında önemli bir fark elde edilmemiş, ancak SY2 ve SY5'in (Bacillus izolatları) sürgün uzunluğu ve fide taze ve kuru ağırlıkları bakımından kontrole kıyasla üstün performans gösterdiği belirlenmiştir.

Keywords

Lolium multiflorum Lam, çimlenme, PGPR, priming

The Effects of Bio-Priming on Seed Germination and Seedling Growth of Italian Ryegrass (Lolium multiflorum Lam.)

Abstract

Seed bio-priming applications with plant growth promoting rhizobacteria (PGPR) have been widely used recently to improve germination and seedling growth. Therefore, the aim of this study was to investigate the effects of bio-priming with different bacterial strains on germination and seedling development of Italian ryegrass seeds. The sterilized seeds of the Elif variety (Lolium multiflorum Lam) were inoculated with nine different bacterial strains belonging to Bacillus species (108 cfu/mL bacterial suspension) for 15 min at 120 rpm and then dried at room temperature. The treated seeds were germinated in petri dishes with 25 seeds between 3 filter papers at 22 ±2 ˚C. The study was carried out in a completely randomized design with three replications. As a result of the study, no significant difference was obtained between the treatments in germination percentage and root length, but it was determined that SY2 and SY5 (Bacillus isolates) showed superior performance compared to the control in terms of shoot length and seedling fresh and dry weights.

Keywords

Germination, Lolium multiflorum Lam, PGPR, priming

References

• Açıkgöz, E., 2021. Yem Bitkileri (Cilt 1). Tarım ve Orman Bakanlığı Bitkisel Üretim Müdürlüğü Yayınları, Ankara. Bashan, Y., Holguin, G., and De-Bashan, L. E., 2004. Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997-2003). Canadian journal of microbiology, 50(8), 521-577.
• Bidabadi, S.S., and Mehralian, M., 2020. Seed bio-priming to improve germination, seedling growth and essential oil yield of Dracocephalum Kotschyi Boiss, an endangered medicinal plant in Iran. Gesunde Pflanzen 72 (1), 17–27.
• Chabbi, N., Chafiki, S., Telmoudi, M., Labbassi, S., Bouharroud, R., Tahiri, A., Mentag, R., El Amri, M., Bendiab, K., Hsissou,, D. 2024. Plant-Growth-Promoting Rhizobacteria Improve Seeds Germination and Growth of Argania spinosa. Plants, 13. https://doi.org/10.3390/ plants13152025
• Chitra, P., and Jijeesh, C.M., 2021. Biopriming of seeds with plant growth promoting bacteria Pseudomonas fluorescens for better germination and seedling vigour of the East Indian sandalwood. New For. 1–13 Deshmukh, A.J., Jaiman, R.S., Bambharolia, R.P., and Patil, V.A., 2020. Seed biopriming-a review. Int. J. Econ. Plant. 7 (1), 038–043.
• Egamberdiyeva, D., 2007. The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Applied soil ecology, 36(2-3), 184-189.
• Erman, M., Çiğ, F., Ceritoglu, M. 2022. Determination of optimum PGPB-priming protocol on germintion and seedling growth in lentil. BSEU Journal of Science, 9 (1): 62-70.
• Gowthamy, U., Selvaraju, P., and Hemalatha, G., 2017. Standardization of seed biopriming with liquid biofertilizers on nnake gourd (Trichosanthes cucumerina). Int. J. Curr. Microbiol. Appl. Sci. 6 (12), 2513–2524.
• Houida, S., Yakkou, L., Kaya, L. O., Bilen, S., Fadil, M., Raouane, M., Harti, A.E., Amghar, S., 2022. Biopriming of maize seeds with plant growth‐promoting bacteria isolated from the earthworm Aporrectodea molleri: effect on seed germination and seedling growth. Letters in Applied Microbiology, 75(1), 61-69.
• Lale, V., Kökten, K., 2020. Bingöl şartlarında bazı İtalyan çimi (Lolium multiflorum Lam.) çeşitlerinin ot verimi ve kalitesinin belirlenmesi. Türk Doğa ve Fen Dergisi, 9(Özel Sayı), 46-50.
• Li, H., Yue, H., Li, L., Liu, Y., Zhang, H., Wang, J., and Jiang, X., 2021. Seed Biostimulant Bacillus sp. MGW9 Improves the Salt Tolerance of Maize During Seed Germination, 11. AMB Express, pp. 1–15.
• Mahmood, A., Turgay, O. C., Farooq, M., and Hayat, R., 2016. Seed biopriming with plant growth promoting rhizobacteria: a review. FEMS Microbiology Ecology, 92, fiw112.
• Mitra, D., Mondal, R., Khoshru, B., Shadangi, S., Mohapatra, P.K.D., and Panneerselvam, P., 2021. Rhizobacteria mediated seed bio-priming triggers the resistance and plant growth for sustainable crop production. Current Research in Microbial Sciences, 2, 100071.
• Miljakovi´ D., Marinkovi´ J., Tamindži´ G., Ðor devi´V., Tintor, B., Miloševi´ D., Ignjatov, M., Nikoli´ Z., 2022. Bio-Priming of Soybean with Bradyrhizobium japonicum and Bacillus megaterium: Strategy to Improve Seed Germination and the Initial Seedling Growth. Plants, 11, 1927. https://doi.org/10.3390/ plants11151927
• Moeinzadeh, A., Sharif-Zadeh, F., Ahmadzadeh, M., and Tajabadi, F. H., 2010. Biopriming of Sunflower (Helianthus annuus L.) Seed with'Pseudomonas fluorescens' for Improvement of Seed Invigoration and Seedling Growth. Australian Journal of Crop Science, 4(7), 564-570.
• Özkan, U., Benlioğlu, B., and Telci Kahramanoğulları, C., 2022. A comparison of germination responses on Italian ryegrass (diploid vs tetraploid) seeds to interactive effects of salinity and temperature. Polish Journal of Environmental Studies, 31(5).
• Pérez-García, L.-A., Sáenz-Mata, J., Fortis-Hernández, M., Navarro-Muñoz, C.E., Palacio-Rodríguez, R., and Preciado-Rangel, P., 2023. Plant-Growth-Promoting Rhizobacteria Improve Germination and Bioactive Compounds in Cucumber Seedlings. Agronomy, 13, 315.
• Pişkin, M., 2007. İtalyan çiminde (Lolium multiflorum Lam.) farklı tohum miktarlarının verim ve bazı verim unsurları üzerine etkileri üzerine araştırmalar. Selçuk Üniversitesi, Yüksek Lisans Tezi. Konya, 54ss.
• Roslan, M.A.M., Zulkifli, N.N., Sobri, Z.M., Zuan, A.T.K., Cheak, S.C., and Abdul Rahman, N. A., 2020. Seed biopriming with P-and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling. PLoS One 15 (7), e0232860 p.
• Salantur, A., Ozturk, A., and Akten, S., 2006. Growth and yield response of spring wheat (Triticum aestivum L.) to inoculation with rhizobacteria. Plant Soil and Environment, 52(3), 111.
• Shaharoona, B., Arshad, M., Zahir, Z. A., and Khalid, A., 2006. Performance of Pseudomonas spp. containing ACC-deaminase for improving growth and yield of maize (Zea mays L.) in the presence of nitrogenous fertilizer. Soil Biology and Biochemistry, 38(9), 2971-2975.
• Snedecor, GW, and Cochran, W.G., 1967. Statistical methods. Iowa State College Press, Iowa, USA. 6th ed., pp: 96.
• TUİK, 2023. Yem bitkileri üretimi. Türkiye İstatistik Kurumu. https://data.tuik.gov.tr/Kategori/GetKategori?p=tarim-111&dil=1 Erişim tarihi: 22.10.2024.
• Yaman, M., Yıldız, E., Sümbül, A., Ercişli, S., Sönmez, O., Güneş, A., ... and Say, A., 2023. The Effect of PGPR Applications on Bioactive Content and Fruit Characteristics of Different Apple Scion–Rootstock Combinations. Erwerbs-Obstbau , vol.65, no.5, 1267-1273.
• Yıldız, E., Yaman, M., Ercişli, S., Sümbül, A., Sönmez, O., Güneş, A., ... and Bozhuyuk, M. R.., 2022. Effects of Rhizobacteria Application on Leaf and Fruit Nutrient Content of Different Apple Scion-Rootstock Combinations. Hortıculturae , vol.8, no.6.
• Yıldız, E., Yaman, M., and Say, A., 2023. Effects of rhızobacterıa applıcatıon on enzyme actıvıty of dıfferent apple scıon–rootstock combınatıons. Current Trends in Natural Sciences , vol.12, no.23, 42-48.
• Widawati, S., and Suliasih, 2018. The effect of plant growth promoting rhizobacteria (PGPR) on germination and seedling growth of Sorghum bicolor L. Moench. In IOP conference series: earth and environmental science (Vol. 166, p. 012022). IOP Publishing.
• Wu, S. C., Cao, Z. H., Li, Z. G., Cheung, K. C., and Wong, M. H., 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma, 125(1-2), 155-166.