Research Article
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The Determination of Chemical and Microbiological Characteristics of Total Mixed Ration Feeds Used in a Dairy Cattle Farm

Year 2025, Volume: 8 Issue: 2, 106 - 114
https://doi.org/10.55257/ethabd.1714116

Abstract

The study was conducted over a 10-month period in a private dairy farm where a Total Mixed Ration (TMR) feeding system was implemented. The research material consisted of TMR samples taken from the mixer wagon and feed bunks. The mixer wagon used in the farm had an average capacity of 1 ton, and the TMR composition included grass hay, straw, triticale, sunflower meal, corn silage, molasses, salt, a vitamin-mineral premix, and dairy concentrate feed. In the collected TMR samples, several analyses were conducted, including pH, dry matter (DM), water-soluble carbohydrates (WSC), and microbiological evaluations such as lactic acid bacteria, yeast, and mold counts. Additionally, during each sampling period, ambient temperature and temperature fluctuations at the feed bunks were recorded. The results of the study highlighted the critical importance of feed bunk monitoring, particularly during periods of high ambient temperatures. These findings emphasize the need for more frequent and careful feed management practices under elevated thermal conditions to maintain feed quality and animal performance.

References

  • Abramson D, Sinha RN, Mills JT, 1980. Mycotoxins and odor formation in moist cereal grain during granary storage. Cereal Chem., 57: 346-351.
  • Akyıldız AR, 1984. Yemler bilgisi laboratuvar kılavuzu. Ankara Üniversitesi Ziraat Fakültesi Yayınları, No: 895. Al-Wadai AS, Al-Othman MR, Mahmoud MA, Abd El-Aziz ARM, 2013. Molecular characterization of Aspergillus flavus and aflatoxin contamination of stored rice grains. African Journal of Microbiology Research, 7(48): 5465–5472.
  • Amaral-Philips DM, Bicudo JR, Turner LW, 2001. Managing the Total Mixed Ration to Prevent Problems in Dairy Cows. Cooperative Extension Service, University of Kentucky, No: 12.
  • Anonim, 1986. The Analysis of Agricultural Material. Reference Book, pp. 427–428, London. Chelkowski J, Manka M, 1983. The ability of fusaria pathogenic to wheat, barley and corn to produce zearalenone. Mycotoxin Research, 1: 354–359.
  • Chen J, Stokes MR, Wallace CR, 1994. Effects of enzyme-inoculant systems on preservation and nutritive value of haycrop and corn silages. Journal of Dairy Science, 77(2): 501–512.
  • Christensen CM, Kaufman HH, 1969. Grain Storage. University of Minnesota Press, Minneapolis. Courtin MG, Spoelstra SF, 1990. A simulation model of the microbiological and chemical changes accompanying the initial stage of aerobic deterioration of silage. Grass and Forage Science, 45: 153–165.
  • Driehuis F, Elferink SO, 1990. The impact of the quality of silage on animal health and food safety: a review. Veterinary Quarterly, 22: 212–216.
  • Dunière L, Sindou J, Chaucheyras-Durand F, Chevallier I, Thévenot-Sergentet D, 2013. Silage processing and strategies to prevent persistence of undesirable microorganisms. Animal Feed Science and Technology, 182(1–4): 1–15.
  • Felton CA, DeVries TJ, 2010. Effect of water addition to a total mixed ration on feed temperature, feed intake, sorting behavior, and milk production of dairy cows. Journal of Dairy Science, 93(6): 2651–2660.
  • Hao W, Wang H, Ning T, Yang F, Xu C, 2015. Aerobic stability and effects of yeasts during deterioration of non-fermented and fermented total mixed ration with different moisture levels. Asian-Australasian Journal of Animal Sciences, 28: 816–826.
  • Heinrichs AJ, Kononoff PJ, 2002. Evaluating particle size of forages and TMRs using the new Penn State Forage Particle Separator. Technical Bulletin DAS 02-42. The Pennsylvania State University, College of Agricultural Sciences, Cooperative Extension.
  • Hu X, Hao W, Wang H, et al., 2015. Fermentation characteristics and lactic acid bacteria succession of total mixed ration silages formulated with peach pomace. Asian-Australasian Journal of Animal Sciences, 28: 502–510.
  • Kebede H, Abbas HK, Fisher DK, Bellaloui N, 2020. Relationship between aflatoxin contamination and environmental and management factors under field conditions. Toxins, 12(11): 729.
  • Kızılşimşek M, Mokhtari NEP, Erol A, Öztürk Ç, Gürkan L, 2016. Laktik asit üretme yeteneklerinin yüksek olduğu bilinen izolatların mısır silajının in vitro gaz üretim değerleri ve yem kalitesi özelliklerine etkileri. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 25 (Özel Sayı 2): 285–288.
  • Koç F, Coşkuntuna L, 2003. Silo yemlerinde organik asit belirlemede iki farklı metodun karşılaştırması. Hayvansal Üretim, 44(2): 37–46.
  • Kononoff PJ, Heinrichs AJ, Buckmaster DR, 2003. Modification of the Penn State Forage and Total Mixed Ration Particle Separator and the Effects of Moisture Content on its Measurement. Journal of Dairy Science, 86: 1858–1863.
  • Kung Jr L, Muck RE, 2015. Silage fermentation and preservation. In: Reynolds JP (Ed.), Dairy Production Medicine, pp. 161–167. Wiley Blackwell.
  • Kung Jr L, Shaver RD, Grant RJ, Schmidt RJ, 2018. Silage review: Interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science, 101(5): 4020–4033.
  • Pahlow G, Muck RE, Driehuis F, Elferink SJO, Spoelstra SF, 2003. Microbiology of ensiling. Silage Science and Technology, 42: 31–93.
  • Parvin S, Nishino N, 2010. Bacterial and fungal communities of non-fermented and fermented total mixed ration silage with or without beet pulp. Letters in Applied Microbiology, 51(6): 604–612.
  • Ranjit NK, Kung Jr L, 2000. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. Journal of Dairy Science, 83(3): 526–535.
  • Seale DR, Pahlow G, Spoelstra SF, Lindgren S, Dellaglio F, Lowe JF, 1990. Methods for the Microbiological Analysis of Silage. Proceedings of the Eurobac Conference, 147, Uppsala. Soysal Mİ, 1993. Biyometrinin Prensipleri (İstatistik I ve II Ders Notları). Yayın No: 95, Ders Kitabı No: 64, Trakya Üniversitesi Tekirdağ Ziraat Fakültesi, Tekirdağ.
  • Soysal Mİ. 1993. Biyometrinin Prensipleri (İstatistik I ve II Ders Notları), Yayın No: 95, Ders Kitabı No: 64, T. Ü. Tekirdağ Ziraat Fakültesi Tekirdağ.
  • Tabacco E, Piano S, Revello-Chion A, Borreani G, 2011. Effect of Lactobacillus buchneri LN4637 and Lactobacillus buchneri LN40177 on the aerobic stability, fermentation products, and microbial populations of corn silage under farm conditions. Journal of Dairy Science, 94: 5589–5598.
  • Wambacq E, Vanhoutte I, Audenaert K, De Gelder L, Haesaert G, De Saeger S, 2016. Fungal presence and mycotoxin contamination in silage: An underestimated risk? Journal of Animal Physiology and Animal Nutrition, 100(4): 703–716.
  • Wang H, Ning T, Hao W, Zheng M, Xu C, 2016. Dynamics associated with prolonged ensiling and aerobic deterioration of total mixed ration silage containing whole crop corn. Asian-Australasian Journal of Animal Sciences, 29: 62–72.
  • Xiong J, Xiong L, Zhou H, Liu Y, Wu L, 2018. Occurrence of aflatoxin B1 in dairy cow feedstuff and aflatoxin M1 in UHT and pasteurized milk in central China. Food Control, 92: 386–390.
  • Zhao J, Dong Z, Li J, Shao T, 2020. Effects of temperature and air exposure time on the fermentation quality and microbial community of total mixed ration silage. Journal of Applied Microbiology, 128(3): 893–904.

Özel BirSüt Sığırcılık İşletmesinde Kullanılan Toplam Rasyon Karışımı Yemlerin Kimyasal ve Mikrobiyolojik Özelliklerinin Belirlenmesi

Year 2025, Volume: 8 Issue: 2, 106 - 114
https://doi.org/10.55257/ethabd.1714116

Abstract

Bu araştırma, toplam rasyon karışımı (TRK) sisteminin uygulandığı özel bir süt sığırcılığı işletmesinde 10 aylık bir süre boyunca gerçekleştirilmiştir. Çalışmanın materyalini, işletmede hazırlanan TRK yemlerinden mikser vagon ve yemliklerden belirli aralıklarla alınan örnekler oluşturmuştur. Araştırmanın yürütüldüğü işletmede mikser vagonun kapasitesi yaklaşık 1 ton olup, TRK; çayır otu, saman, tritikale, ayçiçeği küspesi, mısır silajı, melas, tuz, vitamin-mineral premiksi ve süt yeminden oluşmaktadır. TRK örneklerinde pH, kuru madde (KM), suda çözünebilir karbonhidrat (SÇK) gibi kimyasal analizlerin yanı sıra laktik asit bakteri sayısı, maya ve küf gibi mikrobiyolojik analizler de yapılmıştır. Numune alım dönemlerinde çevre sıcaklığı ve yemliklerdeki sıcaklık değişimleri düzenli olarak kaydedilmiştir. Elde edilen bulgular, özellikle çevre sıcaklığının yüksek olduğu dönemlerde yemlik kontrollerinin daha sık ve dikkatli bir şekilde yapılmasının önemini ortaya koymaktadır.

References

  • Abramson D, Sinha RN, Mills JT, 1980. Mycotoxins and odor formation in moist cereal grain during granary storage. Cereal Chem., 57: 346-351.
  • Akyıldız AR, 1984. Yemler bilgisi laboratuvar kılavuzu. Ankara Üniversitesi Ziraat Fakültesi Yayınları, No: 895. Al-Wadai AS, Al-Othman MR, Mahmoud MA, Abd El-Aziz ARM, 2013. Molecular characterization of Aspergillus flavus and aflatoxin contamination of stored rice grains. African Journal of Microbiology Research, 7(48): 5465–5472.
  • Amaral-Philips DM, Bicudo JR, Turner LW, 2001. Managing the Total Mixed Ration to Prevent Problems in Dairy Cows. Cooperative Extension Service, University of Kentucky, No: 12.
  • Anonim, 1986. The Analysis of Agricultural Material. Reference Book, pp. 427–428, London. Chelkowski J, Manka M, 1983. The ability of fusaria pathogenic to wheat, barley and corn to produce zearalenone. Mycotoxin Research, 1: 354–359.
  • Chen J, Stokes MR, Wallace CR, 1994. Effects of enzyme-inoculant systems on preservation and nutritive value of haycrop and corn silages. Journal of Dairy Science, 77(2): 501–512.
  • Christensen CM, Kaufman HH, 1969. Grain Storage. University of Minnesota Press, Minneapolis. Courtin MG, Spoelstra SF, 1990. A simulation model of the microbiological and chemical changes accompanying the initial stage of aerobic deterioration of silage. Grass and Forage Science, 45: 153–165.
  • Driehuis F, Elferink SO, 1990. The impact of the quality of silage on animal health and food safety: a review. Veterinary Quarterly, 22: 212–216.
  • Dunière L, Sindou J, Chaucheyras-Durand F, Chevallier I, Thévenot-Sergentet D, 2013. Silage processing and strategies to prevent persistence of undesirable microorganisms. Animal Feed Science and Technology, 182(1–4): 1–15.
  • Felton CA, DeVries TJ, 2010. Effect of water addition to a total mixed ration on feed temperature, feed intake, sorting behavior, and milk production of dairy cows. Journal of Dairy Science, 93(6): 2651–2660.
  • Hao W, Wang H, Ning T, Yang F, Xu C, 2015. Aerobic stability and effects of yeasts during deterioration of non-fermented and fermented total mixed ration with different moisture levels. Asian-Australasian Journal of Animal Sciences, 28: 816–826.
  • Heinrichs AJ, Kononoff PJ, 2002. Evaluating particle size of forages and TMRs using the new Penn State Forage Particle Separator. Technical Bulletin DAS 02-42. The Pennsylvania State University, College of Agricultural Sciences, Cooperative Extension.
  • Hu X, Hao W, Wang H, et al., 2015. Fermentation characteristics and lactic acid bacteria succession of total mixed ration silages formulated with peach pomace. Asian-Australasian Journal of Animal Sciences, 28: 502–510.
  • Kebede H, Abbas HK, Fisher DK, Bellaloui N, 2020. Relationship between aflatoxin contamination and environmental and management factors under field conditions. Toxins, 12(11): 729.
  • Kızılşimşek M, Mokhtari NEP, Erol A, Öztürk Ç, Gürkan L, 2016. Laktik asit üretme yeteneklerinin yüksek olduğu bilinen izolatların mısır silajının in vitro gaz üretim değerleri ve yem kalitesi özelliklerine etkileri. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 25 (Özel Sayı 2): 285–288.
  • Koç F, Coşkuntuna L, 2003. Silo yemlerinde organik asit belirlemede iki farklı metodun karşılaştırması. Hayvansal Üretim, 44(2): 37–46.
  • Kononoff PJ, Heinrichs AJ, Buckmaster DR, 2003. Modification of the Penn State Forage and Total Mixed Ration Particle Separator and the Effects of Moisture Content on its Measurement. Journal of Dairy Science, 86: 1858–1863.
  • Kung Jr L, Muck RE, 2015. Silage fermentation and preservation. In: Reynolds JP (Ed.), Dairy Production Medicine, pp. 161–167. Wiley Blackwell.
  • Kung Jr L, Shaver RD, Grant RJ, Schmidt RJ, 2018. Silage review: Interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science, 101(5): 4020–4033.
  • Pahlow G, Muck RE, Driehuis F, Elferink SJO, Spoelstra SF, 2003. Microbiology of ensiling. Silage Science and Technology, 42: 31–93.
  • Parvin S, Nishino N, 2010. Bacterial and fungal communities of non-fermented and fermented total mixed ration silage with or without beet pulp. Letters in Applied Microbiology, 51(6): 604–612.
  • Ranjit NK, Kung Jr L, 2000. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. Journal of Dairy Science, 83(3): 526–535.
  • Seale DR, Pahlow G, Spoelstra SF, Lindgren S, Dellaglio F, Lowe JF, 1990. Methods for the Microbiological Analysis of Silage. Proceedings of the Eurobac Conference, 147, Uppsala. Soysal Mİ, 1993. Biyometrinin Prensipleri (İstatistik I ve II Ders Notları). Yayın No: 95, Ders Kitabı No: 64, Trakya Üniversitesi Tekirdağ Ziraat Fakültesi, Tekirdağ.
  • Soysal Mİ. 1993. Biyometrinin Prensipleri (İstatistik I ve II Ders Notları), Yayın No: 95, Ders Kitabı No: 64, T. Ü. Tekirdağ Ziraat Fakültesi Tekirdağ.
  • Tabacco E, Piano S, Revello-Chion A, Borreani G, 2011. Effect of Lactobacillus buchneri LN4637 and Lactobacillus buchneri LN40177 on the aerobic stability, fermentation products, and microbial populations of corn silage under farm conditions. Journal of Dairy Science, 94: 5589–5598.
  • Wambacq E, Vanhoutte I, Audenaert K, De Gelder L, Haesaert G, De Saeger S, 2016. Fungal presence and mycotoxin contamination in silage: An underestimated risk? Journal of Animal Physiology and Animal Nutrition, 100(4): 703–716.
  • Wang H, Ning T, Hao W, Zheng M, Xu C, 2016. Dynamics associated with prolonged ensiling and aerobic deterioration of total mixed ration silage containing whole crop corn. Asian-Australasian Journal of Animal Sciences, 29: 62–72.
  • Xiong J, Xiong L, Zhou H, Liu Y, Wu L, 2018. Occurrence of aflatoxin B1 in dairy cow feedstuff and aflatoxin M1 in UHT and pasteurized milk in central China. Food Control, 92: 386–390.
  • Zhao J, Dong Z, Li J, Shao T, 2020. Effects of temperature and air exposure time on the fermentation quality and microbial community of total mixed ration silage. Journal of Applied Microbiology, 128(3): 893–904.
There are 28 citations in total.

Details

Primary Language English
Subjects Zootechny (Other)
Journal Section Research Article
Authors

Melek Sezgin This is me 0000-0001-7749-7420

Fisun Koc 0000-0002-5978-9232

Early Pub Date December 2, 2025
Publication Date December 2, 2025
Submission Date June 5, 2025
Acceptance Date August 15, 2025
Published in Issue Year 2025 Volume: 8 Issue: 2

Cite

APA Sezgin, M., & Koc, F. (2025). The Determination of Chemical and Microbiological Characteristics of Total Mixed Ration Feeds Used in a Dairy Cattle Farm. Erciyes Tarım Ve Hayvan Bilimleri Dergisi, 8(2), 106-114. https://doi.org/10.55257/ethabd.1714116