EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY

Abstract

This study aimed to examine the effects of albumin administration on ischemia-reperfusion in the rat ovary by using biochemical, histological, and immunohistochemical methods. Thirty-two Wistar albino rats were used in the study, and they were divided into 4 groups: control, albumin, placebo, and ischemia-reperfusion. Healthy ovaries were taken from the first group. In the other three groups, 2-hour ischemia and 2-hour reperfusion were applied to the bilateral ovaries. In the albumin group, intraperitoneal albumin (2.5 g/kg, 20% human albumin) was administered 30 minutes before reperfusion, and in the placebo group, the same volume of intraperitoneal saline was administered instead of albumin 30 minutes before reperfusion. Ovarian damage scores, cytochrome C-1 immunoreactivity, total oxidant status, total antioxidant status, and oxidative stress index levels were evaluated. In the statistical analysis performed between the groups, it was seen that the results of the control group were significantly lower than the ischemia-reperfusion group in terms of total oxidant status values (p=0.001), and the results of the ischemia-reperfusion group were significantly higher than the control and albumin groups in terms of oxidative stress index values (p<0.001 and p=0.004, respectively). In histological examinations, the total damage score obtained by evaluating follicular degeneration, edema, vascular congestion, and hemorrhage was found to be significantly higher in the ischemia-reperfusion group than in the control group (p=0.003). According to the immunohistochemical examination results, cytochrome C-1 immunoreactivity in the ischemia-reperfusion group was significantly stronger than the control and albumin groups (p<0.001). We think that albumin administration reduces cytochrome C-1, reactive oxygen species, and oxidative stress levels, therefore it will play a helpful role in the ischemia-reperfusion treatment process.

Keywords

• Cytochrome C-1
• Human Serum Albumin
• Immunohistochemistry
• Ischemia-Reperfusion
• Ovary

Supporting Institution

Kafkas Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

2021-TS-93

Thanks

The results described in this article are part of the student thesis (Ali Alper Kahraman's master thesis (2023) at Kafkas University, Institute of Medical Sciences) and this thesis was supported by Kafkas University Scientific Research Projects Coordination Unit (Project No: 2021-TS-93). We would like to thank Dr. Serdar YİĞİT for his contributions to the laboratory processes.

İSKEMİ-REPERFÜZYON HASARLI RAT OVARYUMUNDA ALBÜMİN UYGULAMASININ SİTOKROM C-1 (CYC1) ÜZERİNE ETKİLERİ

Abstract

Bu çalışma, albumin uygulamasının rat ovaryumunda iskemi-reperfüzyon üzerine etkilerini biyokimyasal, histolojik ve immune histokimyasal yöntemlerle incelemeyi amaçladı. Çalışmada 32 adet Wistar albino rat kullanıldı. Hayvanlar 4 gruba ayrıldı: kontrol, albümin, placebo ve iskemi-reperfüzyon. Birinci gruptan sağlıklı overler alındı. Diğer 3 grupta overlere bilateral 2 saatlik iskemi ve 2 saatlik reperfüzyon uygulandı. Albümin grubuna reperfüzyondan 30 dakika önce intraperitoneal albumin (2,5g/kg, %20 human albumin), placebo grubuna reperfüzyondan 30 dakika önce albumin yerine aynı hacimde intraperitoneal salin verildi. Yumurtalık hasar skorları, Sitokrom C-1 immüno reaktivitesi, toplam oksidan durumu, toplam antioksidan durumu ve oksidatif stress indeksi değerleri değerlendirildi. Gruplar arasında yapılan istatistiksel analizlerde toplam oksidan durumu değerleri açısından control grubu sonuçları iskemi-reperfüzyon grubuna gore belirgin olarak düşük olduğu (p=0,001) ve oksidatif stress indeksi değerleri açısından ise iskemi-reperfüzyon grubu sonuçlarının control ve albumin gruplarına gore anlamlı olarak yüksek olduğu görüldü (p<0,001 ve p=0,004, sırasıyla). Histolojik incelemelerde folikül dejenerasyonu, ödem, damar tıkanıklığı ve hemoraji değerlendirilerek elde edilen toplam hasar puanı IR grubunda control grubuna gore anlamlı olarak yüksek bulundu (p=0,003). İmmüno histokimyasal inceleme sonuçlarına gore iskemi-reperfüzyon grubundaki sitokrom C-1 immün reaktivitesinin control ve albumin gruplarına gore anlamlı derecede daha güçlüydü (p<0,001). Albümin uygulamasının sitokrom C-1, oksijen radikalleri ve oksidatif stress düzeylerini düşürdüğünü, bu nedenlei skemi-reperfüzyon tedavi sürecinde yardımcı bir rol oynayacağını düşünüyoruz.

Keywords

• Sitokrom C-1
• İnsan Serum Albümini
• İmmünohistokimya
• İskemi-Reperfüzyon
• Ovaryum

Project Number

2021-TS-93

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