Objectives: The present study compares the clinicopathological features and outcomes of two cystic stump closure techniques (locking clips and intra-corporeal ligation with silk sutures) during laparoscopic cholecystectomy.Methods: This study was conducted in a tertiary health centre as a prospective observational study between October 5, 2021, and February 15, 2022. For randomisation, double rows of silk sutures were used to close the cystic stump in patients on the odd-numbered day of the month (Group 1). In contrast, a single-row locking clip (Hem-o-Lok®) (Group 2) was used to close the cystic stump in patients on the even-numbered day of the month. The study was terminated when at least 50 patients were reached in both groups. The clinicopathological differences and cholecystectomy outcomes were compared between the groups.Results: This observational study included 114 patients. Of the study cohort, 87 (76.3%) were female, and the mean age was 46.54 ± 14.74 years. There were 64 (56.1%) patients in Group 1 and 50 (43.9%) patients in Group 2. Preoperative ERCP requirement was higher in Group 1 (15.6% vs 4%, p = 0.045), while the mean operation room time was longer in Group 2 (p = 0.015). Morbidity rates were similar in the two groups (3.1% vs 2%) (p > 0.05). Conclusions: Cystic stump closure is the essential step of laparoscopic cholecystectomy. According to the present study’s results, silk sutures (intra-corporeal ligation) and locking clips (Hem-o-Lok®) are materials that can be used safely to close a cystic stump.