Okaliptol (1.8-sineol) gentamisin kaynaklı karaciğer hasarını hafifletir

Yıl 2024, Cilt: 35 Sayı: 2, 133 - 141, 03.01.2025

Özhan Karataş , Filiz Kazak , Gökhan Akçakavak , Halil Alakuş , Ahmed Abduljabbar , Ömer Kırgız , İbrahim Alakuş , Bahadır Kılınç , Zeynep Çelik , Mehmet Tuzcu

https://doi.org/10.35864/evmd.1549327

Öz

Aminoglikozid antibiyotik sınıfında yer alan gentamisin (GM), gram negatif bakterilere bağlı hastalıkların tedavisinde günümüzde sıklıkla tercih edilmektedir. Ancak karaciğer ve böbrek fonksiyonları üzerine belirgin yan etkilerinin olması klinik yararlılığını sınırlamaktadır. Okaliptolün (OKA, 1.8-sineol) antioksidan ve antiinflamatuar tıbbi aktiviteleri farklı çalışmalarda bildirilmiştir. Bu çalışmada GM kaynaklı hepatotoksisite üzerine OKA’nın etkilerinin değerlendirilmesi amaçlanmıştır. Çalışma grupları kontrol (K), OKA, GM ve GM + OKA'dan oluşmaktaydı ve her grupta 7 rat bulunmaktaydı. Çalışmanın sonunda ratlar uygun koşullar altında ötenazi edilerek örnekler toplandı ve biyokimyasal, histopatolojik ve immünohistokimyasal analizleri yapıldı. GM grubunda serum alanin aminotransferaz (ALT), aspartat aminotransferaz (AST) ve gama-glutamil transferaz (GGT) enzimlerinde K grubuna kıyasla anlamlı artış olduğu belirlendi (p<0.001). GM uygulamasının karaciğerlerde histopatolojik hasara neden olduğu belirlendi. Ayrıca immünohistokimyasal olarak GM grubundaki ratlarda K grubuna kıyasla 4-hidroksinonenal (4-HNE), 8-hidroksi-2'-deoksiguanozin (8-OHdG) ve malondialdehit (MDA) ekspresyonlarında anlamlı artışa neden oldu (p<0.001). Okaliptol uygulaması (GM+OKA) serum ALT, AST ve GGT enzimlerindeki artışı düzenledi. Ayrıca 4-HNE, 8-OHdG ve MDA ekspresyonlarını azalttı. GM uygulamasının neden olduğu histopatolojik hasarı azalttı. Bu çalışmanın sonuçları OKA'nın GM kaynaklı karaciğer hasarında antioksidan, koruyucu ve tedavi edici etkinlik gösterdiğini ortaya koydu.

Anahtar Kelimeler

gentamisin, hepatotoksisite, MDA, okaliptol (1.8-sineol), 8-OHdG

Eucalyptol (1.8-cineole) attenuates gentamicin-induced liver injury

Öz

Gentamicin (GM), which is in the aminoglycoside antibiotic class, is frequently preferred today in the treatment of diseases caused by gram-negative bacteria. However, its significant side effects on liver and kidney functions limit its clinical usefulness. The antioxidant and anti-inflammatory medical activities of eucalyptol (EUC, 1.8-cineole) have been reported in different studies. This study aimed to evaluate the effects of EUC on GM-induced hepatotoxicity. The study groups are consisted of control (C), EUC, GM and GM + EUC, and there were 7 rats in each group. At the end of the study, the rats were euthanized under appropriate conditions and samples were collected and biochemical, histopathological and immunohistochemical analyzes were performed. It was determined that there was a important increase in serum alanine aminotransferase (ALT), aspartate transferase (AST) and gamma-glutamyl transferase (GGT) enzymes in the GM group relative to the C group (p<0.001). GM application is determined that it caused histopathological damage in livers. Additionally, immunohistochemically, it caused an important increase in the expressions of 4-hydroxynonenal (4-HNE), 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) in rats in the GM group relative to the C group (p<0.001). Eucalyptol application (GM+EUC) regulated the increase in serum ALT, AST and GGT enzymes. It also attenuated 4-HNE, 8-OHdG and MDA expressions. It attenuated the histopathological damage caused by GM application. The results of this study revealed that EUC showed antioxidative, protective and curative efficacy in GM-induced liver damage.

Anahtar Kelimeler

eucalyptol (1.8-cineole), gentamicin, hepatotoxicity, MDA, 8-OHdG

Kaynakça

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