Farmasötik Yumuşak Kapsülde α-Tokoferolün Spektrofotometri ile Kantitatif Tayini

Year 2020, Volume: 10 Issue: 2, 72 - 76, 26.08.2020

Gamze Özgül Artuç

https://doi.org/10.26650/experimed.2020.0009

Abstract

Amaç: E vitamini sağlıklı durumun korunması ve hastalıkların önlenmesi için gerekli bir mikro besin maddesidir. Bu çalışmanın amacı, farmasötik preparatlarda α-tokoferolün belirlenmesi için basit, duyarlı ve kolayca uygulanabilir bir spektrofotometrik yöntem geliştirmek ve doğrulamaktır.

Gereç ve Yöntem: Farmasötik preparatlarda α-tokoferolün kantitatif tayini 290 nm’de ölçülen maksimum absorbans değeri kullanılarak gerçekleştirildi. Kalibrasyon grafikleri, beş farklı konsantrasyonda (10-100 µg/mL) standart α-tokoferol örneklerinin absorbanslarına karşılık gelen konsantrasyonların çizilmesiyle oluşturuldu.

Bulgular: Farmasötik yumuşak kapsüldeki α-tokoferol miktarı %101,572 (203,145 IU/kapsül) olarak hesaplandı (Evicap yumuşak kapsül etiket içeriği: 200 IU/kapsül).

Sonuç: Bu çalışmada geliştirilen spektrofotometrik yöntemin doğru, duyarlı, hassas, tekrarlanabilir olduğu ve yumuşak kapsül ve diğer farmasötik preparatlara kolayca uygulanabileceği ileri sürülmektedir.

Keywords

Spektrofotometri, farmasötik yumuşak kapsül, α-tokoferol

Quantitative Determination of α-Tocopherol in Pharmaceutical Soft Capsule by Spectrophotometry

Abstract

Objective: Vitamin E is an essential micronutrient for maintaining a healthy status and preventing disease. The purpose of this study was to develop and validate a simple, sensitive and easily applicable spectrophotometric method for determination of α-tocopherol in pharmaceutical preparations.

Material and Method: The quantitative determination of the α-tocopherol in pharmaceutical prepation was carried out using the maximum absorbance value measured at 290 nm. Calibration graphs were constructed by plotting the absorbance against the corresponding concentration of standart α-tocopherol samples at five different concentrations (10-100 µg/mL).

Results: The amount of α-tocopherol in the pharmaceutical soft capsule was calculated as 101.572% (203.145 IU/capsule) (Evicap soft capsule labelled content: 200 IU/capsule).

Conclusion: It suggested that the developed spectrophotometric method in this study is accurate, sensitive, precise, reproducible and easily applied to soft capsules and the other pharmaceutical preparations.

Keywords

Spectrophotometry, pharmaceutical soft capsule, α-tocopherol

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