Antibiofilm Effect of Moringa oleifera Leaf Extract Against Staphylococcus aureus, Cytotoxicity, Biochemical aspects, Anti-Inflammatory potential, and Interference on the Activity of Antimicrobial Drugs

Year 2023, Volume: 48 Issue: 2, 241 - 254, 01.08.2023

Lorena Kimberly Silva Alcântara Luiz Felipe Carreiro Machado Isabela Penna Ceravolo Renan Martins Marcus Vinícius Dias Souza

https://doi.org/10.55262/fabadeczacilik.1213447

Abstract

Abstract: There is a growing technical difficulty to treat infectious diseases due to molecular mechanisms of resistance to antimicrobial drugs, such as biofilm formation. Here we provide evidence of the antimicrobial potential of the hydroethanolic extract of Moringa oleifera leaves, traditionally used to treat disorders like cardiovascular and endocrine diseases, on clinical isolates of Staphylococcus aureus. The extract was chemically characterized using classic techniques and by ultra performance liquid chromatography (UPLC). We carried out minimum inhibitory concentration tests, as well as minimal bactericidal concentration and minimal biofilm eradication concentration tests. Moreover, we tested the anti-inflammatory potential and assessed the toxicity of the extract on buffalo green monkey (BGM) cells. We also investigated the effects of combining the extract to clinically relevant antimicrobial drugs (i.e., synergistic or antagonistic interactions). The extract was active at 8 g/mL and 16 g/mL for planktonic cells and biofilms, respectively. Its anti-inflammatory potential was confirmed, and it lacked cytotoxicity. No significant interference of the extract on antimicrobial drugs was observed. Flavonoids, tannins, proteins, carbohydrates and vitamin C were detected in the extract. Our data open doors for further studies with isolated molecules of the extract in order to conduct in vivo antimicrobial tests.

Keywords

Moringa oleifera, Staphylococcus aureus, antimicrobial, biofilms., Moringa oleifera, Staphylococcus aureus, antimicrobial, biofilms.

Supporting Institution

None

Project Number

None

Thanks

We are thankful to Tito Pechir (RVN, Brazil), for kindly providing the leaf powder used in this study, and to Arthur Menezes (Pitágoras College, Brazil), for technical support on the experiments.

References

• AACC – American Association of Cereal Chemists, 2010. Method 08-01.01, 30-20, 44-15-A, 46-13.01,54-60.01, 56-0.
• Abd, N.Z.R., Husain, K., Kumolosasi, E. (2018). Moringa Genus: A Review of Phytochemistry and Pharmacology. Frontiers in Pharmacology, 2018(9),108. doi:10.3389/fphar.2018.00108
• Abril, A.G., Villa, T.G, Barros-Velázquez, J., Cañas, B., Sánchez-Pérez, A., Calo-Mata, P., Carrera, M. (2020). Staphylococcus aureus Exotoxins and Their Detection in the Dairy Industry and Mastitis. Toxins, 12(9), 537. https://doi.org/10.3390/toxins12090537