farabi med j

Farabi Tıp Dergisi

2979-9821

Karadeniz Technical University

Health Care Administration

Sağlık Kurumları Yönetimi

The Effect of Chlorogenic Acid on 5-Fluorouracil-Induced Oxidative Damage in Rat Ovarian Tissue

Klorojenik Asidin Sıçan Yumurtalık Dokusunda 5-Florourasil ile İndüklenen Oksidatif Hasar Üzerine Etkisi

https://orcid.org/0000-0003-2036-5317

Mentese

Ahmet

KARADENIZ TECHNICAL UNIVERSITY

https://orcid.org/0000-0002-1863-6280

Demir

Selim

Karadeniz Teknik Üniversitesi Sağlık Bilimleri Fakültesi Beslenme ve Diyetetik Bölümü

https://orcid.org/0000-0002-8913-8692

Türkmen

Nihal

KARADENIZ TECHNICAL UNIVERSITY

https://orcid.org/0000-0001-9474-4307

Aliyazıcıoğlu

Yüksel

KARADENIZ TECHNICAL UNIVERSITY

https://orcid.org/0000-0003-3584-6324

Değer

Orhan

KARADENIZ TECHNICAL UNIVERSITY

12 30 2022

1 1 1 7 11 06 2022 11 21 2022

2022

Farabi Tıp Dergisi

5-fluorouracil (5-FU) is a potent anticancer agent, but its significant tissue toxicity associated with increased oxidative stress and inflammation may limit its clinical use. Chlorogenic acid (CGA) is a dietary polyphenol found in a variety of foods and beverages, including coffee, apples, pears, strawberries, and grapes. It was aimed to evaluate the therapeutic effects of CGA on 5-FU-induced ovotoxicity through oxidative stress and inflammation parameters in this study. Thirty female Sprague-Dawley rats were divided into 5 groups with equal numbers of subjects (n=6): control, 5-FU (100 mg/kg), 5-FU+CGA (1.5 mg/kg), 5-FU+CGA (mg/kg) and CGA (3 mg/kg). The ovarian tissue levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), 8-hydroxy-2' -deoxyguanosine (8-OHdG), catalase (CAT) and interleukin-6 (IL-6) were determined using commercial spectrophotometric kits. While 5-FU treatment increased MDA, TOS, 8-OHdG and IL-6 levels in ovarian tissue, it significantly decreased TAS and CAT levels (p<0.05). These parameters, indicating 5-FU-induced toxicity, were significantly reversed with CGA administrations in a dose-dependent manner. The results support the view that CGA may be a useful modulator in attenuating 5-FU-induced ovotoxicity.

5-fluorourasil (5-FU) güçlü bir antikanser ajandır, ancak artan oksidatif stres ve inflamasyon ile ilişkili önemli doku toksisitesi klinik kullanımını sınırlayabilmektedir. Klorojenik asit (CGA) kahve, elma, armut, çilek ve üzüm dahil olmak üzere çeşitli yiyecek ve içeceklerde bulunan bir diyet polifenolüdür. Bu çalışmada, CGA'nın 5-FU ile indüklenen yumurtalık dokusu toksisitesi üzerine terapötik etkilerinin oksidatif stres ve inflamasyon parametreleri üzerinden değerlendirilmesi amaçlandı. Otuz Sprague-Dawley ırkı dişi sıçan eşit sayıda denek (n=6) içeren 5 gruba ayrıldı: kontrol, 5-FU (100 mg/kg), 5-FU+CGA (1.5 mg/kg), 5-FU+CGA (mg/kg) ve CGA (3 mg/kg). Yumurtalık dokularında malondialdehit (MDA), toplam oksidan durum (TOS), toplam antioksidan durum (TAS), 8-hidroksi-2'-deoksiguanozin (8-OHdG), katalaz (CAT) ve interlökin-6 (IL-6) seviyeleri ticari spektrofotometrik kitler kullanılarak belirlendi. 5-FU uygulaması yumurtalık dokusunda MDA, TOS, 8-OHdG ve IL-6 düzeylerini artırırken, TAS ve CAT düzeylerini anlamlı olarak azalttı (p<0.05). 5-FU ile indüklenen toksisiteyi gösteren bu parametreler, CGA tedavisi ile doza bağlı bir şekilde anlamlı derecede tersine çevrildi (p<0.05). Sonuçlar, CGA'nın 5-FU ile indüklenen yumurtalık dokusu toksisitesini azaltmada faydalı bir modülatör olabileceği görüşünü desteklemektedir.

5-fluorouracil Chlorogenic acid Inflammation Ovotoxicity Oxidative stress

5-florourasil İnflamasyon Klorojenik asit Yumurtalık dokusu toksisitesi Oksidatif stres

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