Molecular Characterization of Rhizoctonia Species and Anastomosis Groups in Barley Production Areas in Ankara Province

Year 2017, Volume: 46 Issue: 2, 61 - 67, 30.12.2017

Filiz Ünal , M. Erdi Kara

Abstract

Barley (Hordeum vulgare L.) is an important
cereal crop in terms of human and animal nutrition in the world. Rhizoctonia species are a diverse group
of fungi that damage small-grain cereals by rotting the seedlings, roots and
crowns. In order to determine the root rot diseases, surveys were carried out
in barley fields in 2015 growing season in Ankara. 48 barley and 48 soil samples
were collected from barley fields. As a result of isolation from plant and soil
samples collected from barley fields, 28 Rhizoctonia
isolates belonging to 9 anastomosis groups were obtained. The isolates were identified
based on hyphal, colony morphology, anastomosis reaction with known tester
isolates and rDNA-ITS sequence analysis. Multinucleate (MN) Rhizoctonia isolates were grouped into
five AGs as R. solani AG 2-2, AG 4 HG
II, AG 5, Waitea circinata var. circinata, Waitea circinata var. oryzae and Binucleate (BN) Rhizoctonia isolates were grouped into
four AGs as AG D I (R. cerealis), AG
A, AG E and AG I. As a result of pathogenicity tests, AG 2-2, AG 4 HG II, AG 5,
Waitea circinata var. circinata, Waitea circinata var. oryzae
and BN AG D I (R. cerealis) groups
were found to be pathogen on barley and it was determined that the most
virulent group was AG 4 HG II.

Keywords

Rhizoctonia spp., anastomosis group, barley

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