Determination of antioxidant, antimicrobial activities, total phenolic and flavonoid contents of Allium rumelicum, Jurinea kilaea and Peucedanum obtusifolium

Year 2023, Current Perspectives in Modern Biology: Exploring Diverse Frontiers, Paradigms, and Novel Horizons, 1 - 8, 31.12.2023

Ayça Karasakal , Orhan Kılıç , Nazan Tokatlı Demirok , Evren Cabi

https://doi.org/10.51753/flsrt.1365203

Abstract

A microwave-assisted extraction (MAE) process for polyphenols from Allium rumelicum Kocyigit & Ozhatay, Jurinea kilaea Azn. and Peucedanum obtusifolium Sibth. & Sm. was used. This research examined the methanolic extracts made from these three species’ antioxidant, antimicrobial, total phenolic, and flavonoid contents. By using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical method (DPPH), ABTS/Persulfate, and Cupric reducing antioxidant capacity (CUPRAC) methods, the total antioxidant activities and capacities were examined. Additionally, the Folin-Ciocalteu and AlCl3/KAc techniques were used to calculate the total phenolic and flavonoid contents. To ascertain the antibacterial capabilities of plants, the disc diffusion method was applied. The J. kilaea showed the greatest total antioxidant capacity/activity levels when measured using the CUPRAC and ABTS/Persulfate techniques. A. rumelicum was found to have the highest quercetin concentration, while P. obtusifolium had the lowest. In J. kilaea, the gallic acid concentration was highest. The highest antimicrobial activity values were obtained in P. obtusifolium.

Keywords

Antimicrobial activity, antioxidant activity, flavonoid, total phenolic, spectrophotometric method

Supporting Institution

Tekirdağ Namık Kemal University

Project Number

NKUBAP.00.YL.19.226

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