Chromatographic analysis of chemical pollutants in river ecosystems in the northwest of Türkiye

Year 2025, Volume: 6 Issue: 3, 164 - 171

Pınar Altınoluk Mimiroğlu

https://doi.org/10.51753/flsrt.1718398

Abstract

The Meric-Ergene Basin is situated in the Thrace region of northwestern Türkiye, representing a significant hydrological subunit within the country’s transboundary water systems. In addition to intensive agricultural practices within the basin, water pollution originating from both industrial and urban sources constitutes one of the major environmental challenges in the region. In this study, water samples were taken from localities with different sources of pollution in the Meric, Ergene and Tunca River, which are important surface sources of the Meric-Ergene Basin, at seasonal intervals for a year. pH and electrical conductivity were measured using EPA standard methods, while suspended particulate matter was determined with GF/F glass microfiber filters. Heavy metals and other elements were analyzed directly by ICP-MS; anions were analyzed by IC; pesticides, phthalate esters, and bisphenol-A analyses were performed by LC-MS/MS. The analyses employed chromatographic techniques due to their high sensitivity, repeatability, and dependability. As a result, even trace amounts of contaminants could be found. All experimentally obtained data were evaluated in accordance with the criteria set forth in the Water Pollution Control Regulation of Türkiye. Average metal concentrations in the Ergene River, notably those of Co, Cr, Cu, and Fe, were found to surpass regulatory thresholds. Furthermore, Carbendazim, Thiamethoxam, Acetamiprid, Metalaxyl, and Azoxystrobin emerged as the most commonly applied pesticide compounds within the Meric-Ergene River Basin. Bis(2-ethylhexyl) phthalate values were found to be above limits. Bisphenol-A was only found at some stations in the summer. The results of this research provide data to improve water protection and human health monitoring in the Meric-Ergene River Basin.

Keywords

Chromatographic analysis , heavy metal , pesticide , phthalate esters , pollution , water quality

Supporting Institution

Trakya University Scientific Research Projects Unit (Project No: 2022-196)

Project Number

2022/196

Thanks

The Trakya University Scientific Research Projects Unit provided financial support for this work. The experiments comply with the current laws of the country in which they were performed. Also, thanks to Trakya University Technology Research and Development Centre (TUTAGEM). This work was financially supported by the Trakya University Scientific Research Projects Unit (Project No: 2022-196).

References

• Altinoluk, P., Camur-Elipek, B., & Aydogdu, H. (2014). Vertical dynamics of some indicator microorganisms in the Tunca River at Turkish Thrace. Macedonian Journal of Ecology and Environment, 16(1-2), 5-9. https://doi.org/10.59194/MJEE14161-205a
• Bates, B. C., Kundzewicz, Z. W., Wu, S., & Palutikof, J. P. (2008). Climate Change and Water. Technical Paper of the İntergovernmental Panel on Climate Change, (pp 1-210).
• Bellingham, K. (2009). Physicochemical parameters of natural waters. (pp 1-17). Stevens Water Monitoring Systems.
• Bulbul, G., & Camur-Elipek, B. (2017). Investigation of the effects of domestic waste on aquatic bacterial distribution in the Meric River (Edirne, Turkey). Biologija, 63(3), 256-263. https://doi.org/10.6001/biologija.v63i3.3580
• Celen, M., Oruc, H. N., Adiller, A., Tore, G. Y., & Engin, G. O. (2022). Contribution for pollution sources and their assessment in urban and industrial sites of Ergene River Basin, Turkey. International Journal of Environmental Science and Technology, 19(12), 11789-11808. https://doi.org/10.1007/s13762-022-03919-0
• Camur-Elipek, B., Arslan, N., Kirgiz, T., & Oterler, B. (2006). Benthic macrofauna in Tunca River (Turkey) and their relationships with environmental variables. Acta Hydrochimica Hydrobiologica, 34(4), 360-366. https://doi.org/10.1002/aheh.200500631
• Chen, P. (2024). Unlocking policy effects: Water resources management plans and urban water pollution. Journal of Environmental Management, 365, 121642.
• Erkmen, B., & Kolankaya, D. (2006). Determination of organochlorine pesticide residues in water, sediment, and fish samples from the Meric Delta, Turkey. International Journal of Environmental Analytical Chemistry, 86(1-2), 161-169. https://doi.org/10.1080/03067310500247926
• Erol, S., Neven, C., Stanislav, F. B., Ali, K. M., & Mihri, H. (2016). Contamination assessment of ecotoxic metals in recent sediments from the Ergene River, Turkey. Environmental Earth Sciences, 75, 1-11. https://doi.org/10.1007/s12665-016-5855-3
• Ferreira, C. S., Kašanin-Grubin, M., Solomun, M. K., Sushkova, S., Minkina, T., Zhao, W., & Kalantari, Z. (2023). Wetlands as nature-based solutions for water management in different environments. Current Opinion in Environmental Science & Health, 33, 100476.
• Guher, H., & Demir, Y. (2018). Tunca Nehri’nin (Edirne) rotifera faunasi ve komunite yapisi. Suleyman Demirel Universitesi Egirdir Su Urunleri Fakultesi Dergisi, 14(2), 125-137. https://doi.org/10.22392/egirdir.365239
• Halli, M., Sari, E., & Kurt, M. A. (2014). Assessment of arsenic and heavy metal pollution in surface sediments of the Ergene River, Turkey. Polish Journal of Environmental Studies. 23(5), 1581-1590.
• Kandasamy, L., Mahendran, A., Sangaraju, S. H. V., Mathur, P., Faldu, S. V., & Mazzara, M. (2025). Enhanced remote sensing and deep learning aided water quality detection in the Ganges River, India supporting monitoring of aquatic environments. Results in Engineering, 25, 103604. https://doi.org/10.1016/j.rineng.2024.103604
• Kirgiz, T., Camur, B., & Arslan, N. (2005). Preliminary study of Enchytraeidae (Oligochatea) in Tunca River. Proc. Estonion Acad. Sci. Biol. Ecol., 54, 310-314. https://doi.org/10.3176/biol.ecol.2005.4.07
• Lenton, T. M., Rockstrom, J., Gaffney, O., Rahmstorf, S., Richardson, K., Steffen, W., & Schellnhuber, H. J. 2019. Climate tipping points—too risky to bet against. Nature, 575(7784), 592-595.
• Masud, M. M., Shamem, A. S. M., Saif, A. N. M., Bari, M. F., & Mostafa, R. (2025). The role of artificial intelligence in sustainable water management in Asia: a systematic literature review with bibliographic network visualization. International Journal of Energy and Water Resources, 9(1), 247-265. https://doi.org/10.1007/s42108-024-00319-7
• Mia, Y., Islam, A. R. T., Jannat, J. N., Jion, M. M. F., Sarker, A., Tokatli, C., Siddique, A. B., Ibrahim, S. M., & Senapathi, V. (2023). Identifying factors affecting irrigation metrics in the Haor Basin using integrated Shannon's Entropy, fuzzy logic and automatic linear model. Environmental Research, 226, 115688. https://doi.org/10.1016/j.envres.2023.115688
• Nimma, D., Devi, O. R., Laishram, B., Ramesh, J. V. N., Boddupalli, S., Ayyasamy, R., Tirth, V., & Arabil, A. (2025). Implications of climate change on freshwater ecosystems and their biodiversity. Desalination and Water Treatment, 321, 100889. https://doi.org/10.1016/j.dwt.2024.100889
• Onyena, A. P., & Sam, K. (2025). The blue revolution: sustainable water management for a thirsty world. Discover Sustainability, 6(1), 63. https://doi.org/10.1007/s43621-024-00631-6
• Ozkan, N., Moubayed-Breil, J., & Camur-Elipek, B. (2010). Ecological analysis of Chironomid larvae (Diptera, Chironomidae) in Ergene River Basin (Turkish Thrace). Turkish Journal of Fisheries and Aquatic Sciences, 10(1), 93-99. https://doi.org/10.4194/trjfas.2010.0114
• Paul, A., Vignesh, K. S., Sood, A., Bhaumik, S., Singh, K. A., Sethupathi, S., & Chanda, A. (2023). Suspended particulate matter analysis of pre and during covid lockdown using Google Earth Engine Cloud Computing: a case study of Ukai Reservoir. Bulletin of Environmental Contamination and Toxicology, 110(1), 1-7. https://doi.org/10.1007/s00128-022-03638-9
• Rosa, L., & Sangiorgio, M. (2025). Global water gaps under future warming levels. Nature Communications, 16(1), 1192. https://doi.org/10.1038/s41467-025-56517-2
• Routti, H., Harju, M., Luhmann, K., Aars, J., Ask, A., Goksøyr, A., Kovacs, K. M., & Lydersen, C. (2021). Concentrations and endocrine disruptive potential of phthalates in marine mammals from the Norwegian Arctic. Environment International, 152, 106458. https://doi.org/10.1016/j.envint.2021.106458
• Sprenke, K. F., Rember, W. C., Bender, S. F., Hoffmann, M. L., Rabbi, F., & Chamberlain, V. E. (2000). Toxic metal contamination in the lateral lakes of the Coeur d’Alene River valley, Idaho. Environmental Geology, 39, 575-586. https://doi.org/10.1007/s002540050469
• Steffen, W., Rockstrom, J., Richardson, K., Lenton, T. M., Folke, C., Liverman, D., & Schellnhuber, H. J. (2018). Trajectories of the Earth System in the Anthropocene. Proceedings of the National Academy of Sciences, 115(33), 8252-8259. https://doi.org/10.1016/j.watres.2024.122546
• Sun, Y., Wang, D., Li, L., Ning, R., Yu, S., & Gao, N. (2024). Application of remote sensing technology in water quality monitoring: From traditional approaches to artificial intelligence. Water Research, 267, 122546.
• Tas-Divrik, M., & Kirgiz, T. (2018). Macrobentic fauna of the Meric River (Turkish Thrace): Composition of the community as related to water quality. Hidrobiologica, 28(3), 277-285. https://doi.org/10.24275/uam/izt/dcbs/hidro/2018v28n3/tas
• Tokatli, C. (2015). Assessment of the water quality in the Meric River: As an element of the ecosystem in the Thrace Region of Turkey. Polish Journal of Environmental Studies. 24(5), 2205-2211. https://doi.org/10.15244/pjoes/58780
• Tokatli, C. (2019a). Drinking water quality assessment of Ergene River Basin (Turkey) by water quality index: Essential and toxic elements. Sains Malaysiana, 48(10), 2071-2081.
• Tokatli, C. (2019b). Water and sediment quality assessment of the lifeblood of Thrace Region (Turkey): Meric River Basin. Fresenius Environmental Bulletin, 28(5), 4131–4140.
• Tokatli, C., Kose, E., Cicek, A., & Emiroglu, O. (2020). Pesticide accumulation in Turkey’s Meric River basinwater and sediment. Polish Journal of Environmental Studies, 29(1), 1003-1008. https://doi.org/10.15244 /pjoes/101618
• Tuji, A., Jacobs, S. M., Malgas, R. R., Dzama, K., & Alamirev, T. (2024). Effects of land-use change on the provisioning ecosystem service of wetlands: the case of a social-ecological systems perspective of Boyo Wetland in Ethiopia. Afr. J. Aquat. Sci. 49, 1–10. https://doi. org/10.2989/16085914.2024.2319670
• Turan, E., & Bayrakdar, E. (2020). Türkiye’nin su yonetim politikalari: Ulusal guvenlik acisindan bir degerlendirme. Uluslararasi Politik Arastirmalar Dergisi, 6(2), 1-19.
• TWPCR, (2016). Turkish Water Pollution Control Regulation. Official Gazette:10.08.2016, 29797, Turkey (in Turkish).
• TWPCR, (2021). Turkish Water Pollution Control Regulation. Official Gazette:16.06.2021, 31513, Turkey (in Turkish)
• Ulusoy, A., Atılgan, A., Rolbiecki, R., Jagosz, B., & Rolbiecki, S. (2024). Innovative approaches for sustainable wastewater resource management. Agriculture, 14(12), 2111.
• US Environmental Protection Agency, EPA, (1993). Water quality criteria. US Environmental Protection Agency, Office of Water, Health and Ecological Criteria Division, Washington, D.C.
• US Environmental Protection Agency, Method 120.1, (1982). Methods for chemical analysis of water and wastes. Office of Research and Development, Specific conductance in water.
• US Environmental Protection Agency, Method 150.1, (1982). Methods for chemical analysis of water and wastes. Office of Research and Development, pH in water by electrometric method.
• US Environmental Protection Agency, Method 200.8, Creed, J. T., Brockhoff, C. A., Martin, T. D. (1994). Determination of trace elements in waters and wastes by inductively coupled plasma-mass spectrometry, Revision 5.4; Environmental Monitoring Systems Laboratory Office of Research and Development, U.S., Cincinnati, Ohio.
• US Environmental Protection Agency, Method 300.1, Hautman, D. P., Munch, D. J. (1997). Determination of inorganic anions in drinking water by ion chromatography, Revision 1.0; National Exposure Research Laboratory, U.S., Cincinnati, Ohio.
• Ulke, A., Ozkul, S., & Tayfur, G. (2011). Ampirik yontemlerle Gediz Nehri icin askida kati madde yuku tahmini. Teknik Dergi. 22(107), 5387-5407.
• Wang, M., Bodirsky, B. L., Rijneveld, R., Beier, F., Bak, M. P., Batool, M., Droppers, B., Popp, A., Vliet, M. T. H., & Strokal, M. (2024). A triple increase in global river basins with water scarcity due to future pollution. Nature Communications, 15(1), 880. https://doi.org/10.1038/s41467-024-44947-3
• Weizhen, Z., Xiaowei, Z., Peng, G., Ning, W., Zini, L., Jian, H., & Zheng, Z. (2020). Distribution and risk assessment of phthalates in water and sediment of the Pearl River Delta. Environmental Science and Pollution Research, 27, 12550-12565. https://doi.org/10.1007/s11356-019-06819-y
• Wu, X., Nawaz, S., Li, Y., & Zhang, H. (2024). Environmental health hazards of untreated livestock wastewater: potential risks and future perspectives. Environmental Science and Pollution Research, 31(17), 24745-24767. https://doi.org/10.1007/s11356-024-32853-6
• Zeng, X., Zhang, H., Zhou, B., Liang, X., Cui, L., Li, H., Qu, Y., & Luo, C. (2024). Hydrological dynamics and its impact on wetland ecological functions in the Sanjiang Plain, China. Ecological Indicators, 169, 112878. https://doi.org/10.1016/j.ecolind.2024.112878
• Zhang, H., Zhou, Q., Xie, Z., Zhou, Y., Tu, C., Fu, C., Mi, W., Ebinghous, R., Christie, P., & Luo, Y. (2018). Occurrences of organophosphorus esters and phthalates in the microplastics from the coastal beaches in north China. Science of the Total Environment, 616, 1505-1512. https://doi.org/10.1016/j.scitotenv.2017.10.163