genel tıp derg Genel Tıp Dergisi 2602-3741 Selcuk University 10.54005/geneltip.1264527 Clinical Sciences Klinik Tıp Bilimleri Investigation of Cytokines, Biochemical Parameters and Oxidative Stress Levels in Serum of Patients with Acute Pancreatitis Akut Pankreatitli Hastaların Serumlarında Sitokinler, Biyokimyasal Parametreler ve Oksidatif Stres Düzeylerinin Araştırılması https://orcid.org/0000-0002-5745-9678 Bayraktar Nihayet HARRAN ÜNİVERSİTESİ https://orcid.org/0000-0001-8497-7625 Aslan Oruç HARRAN ÜNİVERSİTESİ https://orcid.org/0000-0003-4881-5244 Uyanıkoğlu Ahmet HARRAN ÜNİVERSİTESİ https://orcid.org/0000-0003-2428-1831 Öztürk Ali NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ https://orcid.org/0000-0003-2306-6531 Bayraktar Mehmet HARRAN ÜNİVERSİTESİ https://orcid.org/0000-0002-5025-2367 Erdoğdu Hamza HARRAN ÜNİVERSİTESİ 04 30 2024 34 2 158 165 03 13 2023 01 08 2024 Copyright © 1990, Genel Tıp Dergisi 1990 Genel Tıp Dergisi

Objective: This study aimed to find new serum biochemistry parameters, especially for the early identification of severe AP. In the study serum cytokine levels (TNF-A, IL-1, IL-6, IL-10, IL-21), biochemical parameters (Aquaporin-1, Hepcidine, Iron, Zinc, Copper, Nitric Oxide, C-Reactive protein), and oxidative stress parameters analysis were performed.Method: A total of 46 patients with AP and 46 healthy controls were included in this study. Serum cytokine levels, biochemical, and oxidative stress parameters by the ELISA method, and CRP by the immunoturbidimetric method were measured.Results: When patients with AP and control groups were compared, all studied parameters were found to be statistically significant (p<0.05). However, IL-1 was found to be insignificant (p>0.05). Therefore, the Mann-Whitney U test, which is a non-parametric test, was found suitable to determine whether there was a significant difference between the patient and control groups.Conclusions: Investigating the role of cytokines, oxidative stress and other biochemical parameters in the pathogenesis and course of the disease may contribute to a better understanding of the disease process and its therapeutic value. It showed a significant increase in oxidative stress parameters and biochemical parameters such as aquaporin-1, hepcidin, lipase, and amylase, which may help in the diagnosis of AP.

Amaç: Bu çalışma, özellikle şiddetli AP'nin erken teşhisi için yeni serum biyokimya parametreleri bulmayı amaçladı. Çalışmada serum sitokin seviyeleri (TNF-A, IL-1, IL-6, IL-10, IL-21), biyokimyasal parametreler (Aquaporin-1, Hepsidin, Demir, Çinko, Bakır, Nitrik Oksit, C-Reaktif protein) ve oksidatif stres parametreleri analizi yapıldı.Yöntem: Bu çalışmaya AP'li toplam 46 hasta ve 46 sağlıklı kontrol dahil edildi. ELISA yöntemi ile serum sitokin düzeyleri, biyokimyasal ve oksidatif stres parametreleri, immünotürbidimetrik yöntemle de CRP ölçüldü.Bulgular: AP'li hastalar ve kontrol grubu karşılaştırıldığında çalışılan tüm parametreler istatistiksel olarak anlamlı bulundu (p<0.05). Ancak IL-1'in önemsiz olduğu bulundu (p>0.05). Bu nedenle hasta ve kontrol grupları arasında anlamlı fark olup olmadığını belirlemek için parametrik olmayan bir test olan Mann-Whitney U testi uygun bulunmuştur.Sonuç: Sitokinlerin, oksidatif stresin ve diğer biyokimyasal parametrelerin hastalığın patogenezindeki ve seyrindeki rolünün araştırılması, hastalık sürecinin ve tedavi edici değerinin daha iyi anlaşılmasına katkı sağlayabilir. AP tanısında yardımcı olabilecek aquaporin-1, hepsidin, lipaz ve amilaz gibi oksidatif stres parametrelerinde ve biyokimyasal parametrelerde önemli bir artış gösterdi.

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