genel tıp derg Genel Tıp Dergisi 2602-3741 Selcuk University 10.54005/geneltip.1781851 Clinical Sciences (Other) Klinik Tıp Bilimleri (Diğer) Küçük Hücreli Dışı Akciğer Kanseri Hücrelerinde Kurkumin ve Piperin Kombinasyonunun Antikanser Etkisi The Anticancer Effect of Curcumin and Piperine Combination in Non-Small Cell Lung Cancer Cells https://orcid.org/0000-0002-1928-7158 İşitez Nilay AFYONKARAHİSAR SAĞLIK BİLİMLERİ ÜNİVERSİTESİ https://orcid.org/0000-0002-3626-7730 Cigerci İbrahim Hakkı AFYON KOCATEPE ÜNİVERSİTESİ https://orcid.org/0000-0002-2702-6847 Hazman Ömer AFYON KOCATEPE ÜNİVERSİTESİ https://orcid.org/0000-0001-6082-0818 Okumuş Nurullah Sağlık Bilimleri Üniversitesi 04 09 2026 36 2026 1 7 09 11 2025 02 26 2026 Copyright © 1990, Genel Tıp Dergisi 1990 Genel Tıp Dergisi

Amaç: Kurkuminin farklı kanser türlerinde antikanser etkileri olduğu bilinmektedir, ancak zayıf emilimi ve hızlı metabolizması nedeniyle biyoyararlanımı düşüktür. Piperinin, kurkumin gibi birçok terapötik ajanın biyoyararlanımını artırdığı bilinmektedir. Bu çalışmada, A549 küçük hücreli dışı akciğer kanseri hücre hattında kurkumin ve piperin kombinasyon tedavisinin olası sinerjik etkisinin araştırılması amaçlandı.Gereç ve Yöntemler: IC50 dozları MTT tekniği ile belirlendikten sonra, deney gruplarında apoptoz ve hücre döngüsünde rol oynayan genlerin (p53, TRAIL-1, TRAIL-2, NF-κB, IKKB, Bax ve Bcl-2) mRNA ekspresyon düzeyleri ile oksidatif stres, DNA hasarı ve aktif maddelerin inflamasyon (TNF-α, TGF-β, IL-1β ve DEF-β2) üzerindeki etkileri belirlendi.Bulgular: Sonuçlar, kurkumin ve piperinin doza bağımlı olarak hücre canlılığını azalttığını gösterdi. Kurkumin ve piperinin birlikte uygulanması sonucunda DNA hasarı düzeylerinin kontrol grubu ile aynı olduğu; oksidatif stres parametrelerinden, TAS düzeyinin kontrol grubuna göre yüksek olduğu, TOS ve OSI düzeylerinin ise düşük olduğu belirlendi. Ayrıca, inflamatuvar sitokinlerin (TNF-α, TGF-β, IL-1β ve DEF-β2) düzeylerinin de azaldığı görüldü. Moleküler analiz sonuçlarında da, kurkumin ve piperin kombinasyonunun p53, Bax, TRAIL-2, NF-kB mRNA ekspresyon düzeylerini indüklediği, TRAIL-1, IKKB ve Bcl-2 mRNA ekspresyon düzeylerini ise baskıladığı görüldü.Sonuçlar: Bu sonuçlar, kurkumin ve piperin kombinasyonunun küçük hücreli dışı akciğer kanseri tedavisinde mevcut tedavilere adjuvan tedavi yaklaşımı olarak değerlendirilebileceğini düşündürmektedir.

Aim:Curcumin is known to have anticancer effects in different types of cancer, but its bioavailability is low due to its poor absorption and rapid metabolism. Piperine is known to increase the bioavailability of numerous therapeutic agents such as curcumin. This study aimed to investigate the possible synergistic effect of combined curcumin and piperine treatment in the A549 non-small cell lung cancer cell line. Materials and Methods: After IC50 doses were determined using the MTT technique, the mRNA expression levels of genes involved in apoptosis and the cell cycle (p53, TRAIL-1, TRAIL-2, NF-κB, IKKB, Bax, and Bcl-2), as well as the effects of oxidative stress, DNA damage, and inflammation (TNF-α, TGF-β, IL-1β and DEF-β2) of the active substances were determined in the experimental groups.Results:The results showed that curcumin and piperine dose-dependently reduced cell viability. DNA damage levels were the same as in the control group when curcumin and piperine were administered together. TAS level was found to be high compared to the control group, while TOS and OSI levels were low. It was also observed that inflammatory cytokines (TNF-α, TGF-β, IL-1β, and DEF-β2) decreased. Molecular analysis results also showed that the combination of curcumin and piperine up-regulated p53, Bax, TRAIL-2, NF-κB mRNA expression levels, while down-regulated TRAIL-1, IKKB, and Bcl-2 mRNA expression levels.Conclusions:These results suggest that the combination of curcumin and piperine could be considered as an adjuvant treatment approach to current therapies in the treatment of non-small cell lung cancer.

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