genel tıp derg Genel Tıp Dergisi 2602-3741 Selcuk University 10.15321/GenelTipDer.2021.295 Clinical Sciences Klinik Tıp Bilimleri Investigating of Effects of Metformin on Melanoma Skin Cancer Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması Alp Harun Hatay Mustafa Kemal University, Tayfur Ata Sokmen Faculty, Medical Pharmacology Department Tutun Hidayet Burdur Mehmet Akif Ersoy University, Veterinary Faculty, Pharmacology and Toxicology Department Altıntaş Levent Ankara Üniversitesi Veteriner Fakültesi Farmakoloji ve Toksikoloji Anabilim Dalı Kaplan Halil Mahir Cukurova University, Medicine Faculty, Pharmacology Department Şingirik Ergin Cukurova University, Medicine Faculty, Medical Pharmacology Department Kahraman İbrahim Hatay Mustafa Kemal University, Tayfur Ata Sokmen Faculty, Biophysics Department 06 30 2021 31 2 111 115 09 20 2019 07 06 2020 Copyright © 1990, Genel Tıp Dergisi 1990 Genel Tıp Dergisi

Aim: Apoptotic pathways are important for resistance to chemotherapeutics drugs and cancer development. Metformin is a safe, inexpensive and effective drug used to treat type 2 diabetes. Furthermore, although there is evidence of anticancer effects, the mechanisms by which metformin exerts this effect are unclear. If metformin is thought to be safe, cheap and effective drugs, the potentially beneficial effects of these drugs are of great importance. Therefore, it was aimed to investigate Bcl-2, kaspaz-3, bax, wee-1, AIF, grp78 and gadd153 levels in metformin treated skin cancer cells.Materials and Methods: For this propose we prepared culture of skin cancer cells then these cells were treated with metformin and it was analyzed kaspase-3, bcl-2, wee 1, gadd153, grp78 and AIF protein levels by ELISA on these cells.Result: Treatment of metformin increased of proapoptotic proteins (kaspase-3, bcl-2, wee 1, gadd153, grp78 and AIF) and decreased antiapoptotic protein (bcl-2).Conclusion: In conclusion, our study showed that metformin has apoptotic effect on melanoma skin cancer.

Amaç: Kanserlerde kemoterapötiklere gösterilen dirençte ve kanser gelişiminde apoptozisin önemli rolü vardır. Metformin tip 2 diyabet tedavisinde kullanılan güvenli, ucuz ve etkili bir ilaçtır. Ayrıca, antikanser etkilerinin olduğuna dair kanıtlar olmasına rağmen metforminin bu etkiyi hangi mekanizmalar ile gerçekleştirdiği açık değildir. Bu nedenle, çalışmamızda cilt kanseri hücrelerine metformin uygulamasının Bcl-2, kaspaz-3, bax, wee-1, AIF, grp78 ve gadd153 düzeyleri üzerine etkileri araştırıldı.Gereç ve Yöntem: İlgili protein düzeylerinin belirlenmesinde ELISA testleri kullanıldı.Bulgular: Çalışmamızda metforminin cilt kanseri hücrelerinde apoptozis proteinlerini (kaspaz-3, wee 1, gadd153, grp78 ve AIF) arttırdığı ve antiapoptotik markırı (bcl-2) azalttığı belirlendi.Sonuç: Sonuç olarak çalışmamız; metforminin melanom cilt kanseri üzerine apoptotik etkisinin olduğunu göstermiştir.

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