the journal of food Gıda 1300-3070 1309-6273 The Association of Food Technology 10.15237/gida.GD25133 Food Sustainability Food Technology Gıda Sürdürülebilirliği Gıda Teknolojileri MYRTUS COMMUNIS L. YAPRAKLARINDAN BİYOAKTİF BİLEŞİKLERİN EKSTRAKSİYONUNDA DERİN ÖTEKTİK ÇÖZÜCÜLERİN KULLANIMI: TOPLAM FENOLİK İÇERİK, ANTİOKSİDAN AKTİVİTE VE PROSES SÜRDÜRÜLEBİLİRLİĞİNİN DEĞERLENDİRİLMESİ APPLICATION OF DEEP EUTECTIC SOLVENTS FOR EXTRACTION OF BIOACTIVE COMPOUNDS FROM MYRTUS COMMUNIS L. LEAVES: ASSESSING TOTAL PHENOLICS, ANTIOXIDANT ACTIVITY, AND PROCESS SUSTAINABILITY https://orcid.org/0000-0002-4215-2155 Kalkan Ezgi GAZİANTEP ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ, GIDA MÜHENDİSLİĞİ BÖLÜMÜ, GIDA MÜHENDİSLİĞİ PR. (İNGİLİZCE) https://orcid.org/0000-0001-6313-897X Maksan Medeni GAZIANTEP UNIVERSITY, FACULTY OF ENGINEERING, DEPARTMENT OF FOOD ENGINEERING (ENGLISH), FOOD ENGINEERING PR. (ENGLISH) 03 31 2026 51 2 318 326 10 17 2025 01 27 2026 Copyright © 1976, The Journal of Food 1976 The Journal of Food

Bu çalışmada, Myrtus communis L. yapraklarından biyoaktif bileşiklerin ekstraksiyonu için yeşil bir ekstraksiyon yöntemi araştırılmıştır. Ekstraksiyon ortamı olarak, seçilen yedi adet kolin klorür (ChCl) bazlı derin ötektik çözücü (ChCl: Etilen glikol, ChCl: Dietilen glikol, ChCl: Trietilen glikol, ChCl: Oksalik asit, ChCl: Laktik asit, ChCl: Gliserol, ChCl: Üre) kullanılmış ve bunlar geleneksel çözücüler olan metanol, etanol ve etil asetat ile karşılaştırılmıştır. Elde edilen ekstraktlar toplam fenolik madde içeriği (TPC) ve radikal süpürme aktivitesi (RSA) açısından değerlendirilmiştir. TPC bakımından en yüksek değer metanolle elde edilmiş, bunu çok yakın bir biçimde ChCl:Trietilen glikol takip etmiştir. RSA açısından ise en yüksek antioksidan aktivite ChCl:Oksalik asit ile elde edilmiş, bunu ChCl:Etilen glikol izlemiştir. Ayrıca önerilen yeşil yaklaşım AGREEprep metrikleri ile değerlendirilmiştir. Çalışmanın sonuçları, bu yöntemin yeşil kimyanın temel ilkeleriyle uyumlu olduğunu ve sunulan yöntemin AGREEprep skorunun 0.79, geleneksel yaklaşım için ise 0.37 olduğunu göstermiştir. Sonuç olarak, bu çalışma Myrtus communis L. yapraklarından önemli miktarda antioksidanca yüksek fenolik bileşiklerin ekstraksiyonu için yeşil ve umut vadeden bir yöntem ortaya koymuştur.

In this study, a green extraction method for extracting bioactive chemicals from Myrtus communis L. leaves was explored. Seven selected Choline chloride (ChCl)-based deep eutectic solvents (ChCl: Ethylene glycol, ChCl: Diethylene glycol, ChCl: Triethylene glycol, ChCl: Oxalic acid, ChCl: Lactic acid, ChCl: Glycerol, ChCl: Urea) were utilized as extraction medium and compared with traditional solvents, specifically methanol, ethanol, and ethyl acetate. The extracts were assessed for total phenolicc content (TPC) and radical scavenging activity (RSA). In terms of TPC, methanol produced the highest value, closely followed by ChCl:Triethylene glycol. In terms of RSA, ChCl:Oxalic acid had the highest antioxidant activity, followed by ChCl:Ethylene glycol. Additionally, the proposed green approach was evaluated with AGREEprep metrics. The results of the study validated that it was in line with the fundamentals of green chemistry and achieving a score of 0.79 for the present approach in contrast to 0.37 for the conventional approach. Overall, this study revealed a green and promising method for extracting considerable amounts of antioxidative phenolic compounds from Myrtus communis L. leaves

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