the journal of food

Gıda

1300-3070 1309-6273

The Association of Food Technology

10.15237/gida.GD18041

B. CEREUS BİYOFİLMLERİNİN SİTRİK ASİT UYGULAMALARI İLE KONTROLÜ

CONTROL OF B. CEREUS BIOFILMS BY CITRIC ACID TREATMENTS

Yesilcimen Akbas

Meltem

GEBZE TEKNİK ÜNİVERSİTESİ, TEMEL BİLİMLER FAKÜLTESİ Şar

Taner

GEBZE TEKNİK ÜNİVERSİTESİ, TEMEL BİLİMLER FAKÜLTESİ

06 15 2018

43 4 605 616 03 29 2018 06 04 2018

1976

The Journal of Food

Bu çalışmada, önemli bir gıda patojeni olan B. cereusvejetatif hücrelerinin ve sporlarının mikrotitrasyon plaklarında, glukoz ve sütiçeren besiyerleri kullanılarak (TSBG ve TSBS)oluşturduğu 24-72 saatlik biyofilmlerinin, %2 sitrik asit ve 200 ppm kloruygulamaları ile önlenmesi ve ortadan kaldırılması araştırılmıştır. B.cereus vejetatif hücrelerinin TSBG ve TSBSbesiyerleri kullanıldığında oluşan biyofilmlerinin, sitrik asit uygulamalarıile %59 oranında önlendiği ve %38-63 oranlarında giderildiği belirlenmiştir. B.cereus spor biyofilmlerinin ise, %56 oranında önlenebildiği ve %40-56oranlarında giderilebildiği görülmüştür. Sitrik asit uygulamasının (%2) oluşan B.cereus vejetatif hücrelerinin ve sporlarının biyofilmleri üzerinde klorkadar etkili olabildiği tespit edilmiştir.

In this work, the prevention and removal of biofilmformations of B. cereus vegetative cells and spore formations by citricacid (2%) and chlorine (200 ppm) treatments on microtitration plates wereinvestigated. The biofilms were produced in the presence of glucose and milk(TSBG and TSBM) for 24-72 hours. B. cereusbiofilms, formed by vegetative cells in TSBG and TSBMwere inhibited by citric acid treatments by up to 59% and removed about 38-63%,respectively. However, biofilms of B. cereus spores were prevented about56% and removed by 40-56%. It was shown that citric acid treatment (2%) couldbe as effective as chlorine treatment for biofilms of B. cereusvegetative cells and spores.

B. cereus biyofilm önlenmesi biyofilm ortadan kaldırılması sitrik asit B. cereus sporları

prevention of biofilm removal of biofilm citric acid B. cereus spores of B. cereus

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