TİMOL ENKAPSÜLASYONU İÇİN TASARLANMIŞ KİTOSAN NANOPARTİKÜLLER

Year 2024, Volume: 49 Issue: 6, 1148 - 1161, 09.12.2024

Dılhun Keriman Arserim-uçar

https://doi.org/10.15237/gida.GD24055

Abstract

Bu çalışmada, kitosan timol nanopartikülleri elektrohidrodinamik teknik kullanılarak tek adımlı, basit bir prosedür ile başarı ile elde edilmiştir. Elektrosprey timol yüklü kitosan nanopartiküllerin (KTNP’ler) morfolojik ve fiziksel karakterizasyonu, antioksidan ve antimikrobiyal aktivite değerlendirmeleri gerçekleştirilmiştir. KTNP örneklerinin antioksidan ve antimikrobiyal aktivitelerini belirlemek için sırasıyla ABTS yöntemi ve agar well difüzyon testi kullanılmıştır. Analiz sonuçları, KTNP’lerin B. cereus, S. aureus, E. coli ve S. typhimurium'a karşı etkili antimikrobiyal aktiviteye sahip olduğunu göstermiştir. KTNP’ler ABTS yöntemine göre % 90 radikal süpürme aktiviteye sahip oldukları görülmüştür. Biyolojik aktiviteye sahip KTNP’lerin gıda güveliği ve sağlık alanındaki pratik uygulamalarda etkili bir alternatif olabileceği görülmüştür. Bu çalışmada, biyopolimer nanopartiküller üretmek için elektrohidrodinamik atomizasyon tekniğinin kullanılması, timol benzeri uçucu aktif bileşenlerin enkapsülasyonu için yeni bir yaklaşım sunmaktadır.

Keywords

Kitosan, timol, elektrohidrodinamik teknik, partikül, gıda güvenliği

Thanks

The author would like to thank the Integrated Research Center of Izmir Institute of Technology for SEM analysis and Bingöl University, the Center of Research and Application Center for characterization studies.

ENGINEERED CHITOSAN NANOPARTICLES FOR ENCAPSULATION OF THYMOL

Abstract

This study successfully obtained chitosan thymol nanoparticles using an electrohydrodynamic technique, which is a simple one-step procedure. The morphological and physical characterization, antioxidant, and antimicrobial activity assessments of electrosprayed thymol-loaded chitosan nanoparticles (CTNPs) were carried out. The ABTS assay and the agar well diffusion test were used to determine the antioxidant and antimicrobial activities of the CTNP samples, respectively. The results showed that CTNPs possessed efficient antimicrobial capacity against B. cereus, S. aureus, E. coli, and S. typhimurium. CTNPs indicated a radical scavenging activity of 90% regarding the ABTS assay. CTNPs with biological activities could be an effective alternative for practical food safety and health applications. In this study, the use of electrohydrodynamic atomization technique to produce biopolymer nanoparticles present a novel approach for encapsulating thymol-like volatile active agents.

Keywords

Chitosan, thymol, electrohydrodynamic technique, particles, food safety

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