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The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells

Year 2024, Volume: 37 Issue: 1, 16 - 28, 01.03.2024

Merve Güneş , Burcin Yalcin , Ayşen Yağmur Kurşun , Ghada Tagorti , Emre Yavuz Esin Akarsu , Nuray Kaya , Bülent Kaya

https://doi.org/10.35378/gujs.1218829

Abstract

Biomaterials are engineered products that are widely used in many areas of medicine fields such as orthopaedic applications, facial and maxillofacial surgery, artificial heart parts, metal parts, and implantable devices. These materials are widely used in medicine because they are biocompatible with the organism, non-allergic, and are resistant to physical and chemical factors. Hydroxyapatites are bioactive calcium phosphate ceramics that are compatible with tissues. Nano-sized hydroxyapatite has been produced to increase their bioactivity. Although there are advantages to the use of nanoparticles in medicine and therapy, the potential toxicity of these compounds on the ecosystem and human health are of concern. One of the key issues to be investigated is whether the different forms of the same nanoparticle will cause differences in genotoxicity. Herein, the potential genotoxic effects of rod and round forms of nano-sized hydroxyapatites (nHAs) were evaluated using the Allium cepa Single Cell Gel Electrophoresis (SCGE) method. Results had shown that the round form of nHA in the A. cepa meristem root tip cells caused statistically significant genotoxicity at 25 µg/mL concentration in terms of tail intensity and tail moment. This study indicated small-sized-nanohydroxyapatite-induced genotoxicity and cell death in A. cepa. This study has shown that the physical properties of nanoparticles affect potential toxicity mechanisms.

Keywords

Allium cepa DNA damage Genotoxicity Root growth

Supporting Institution

Scientific Research Projects Coordination Unit of Akdeniz University

Project Number

FBA-2018-3285

Thanks

This study is supported by the Scientific Research Projects Coordination Unit of Akdeniz University (Project ID: FBA-2018-3285).

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Year 2024, Volume: 37 Issue: 1, 16 - 28, 01.03.2024

Merve Güneş , Burcin Yalcin , Ayşen Yağmur Kurşun , Ghada Tagorti , Emre Yavuz Esin Akarsu , Nuray Kaya , Bülent Kaya

https://doi.org/10.35378/gujs.1218829

Abstract

Project Number

FBA-2018-3285

References

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  • [3] Ünal, F., Demırtaş Korkmaz, F., Suludere, Z., Erol, Ö., Yüzbaşıoğlu, D., “Genotoxicity of Two Nanoparticles: Titanium Dioxide and Zinc Oxide”, Gazi University Journal of Science, 34(4); 948-958, (2021). https://doi.org/10.35378/gujs.826911
  • [4] Behzadi, S., Serpooshan, V., Tao, W., Hamaly, M.A., Alkawareek, M.Y., Dreaden, E.C., Brown, D., Alkilany, A.M., Farokhzad, O.C., Mahmoudi, M., “Cellular uptake of nanoparticles: journey inside the cell”, Chemical Society Reviews, 46(14): 4218-4244, (2017). https://doi.org/10.1039/C6CS00636A
  • [5] Huang, Y., Cambre, M., Lee, H., “The Toxicity of Nanoparticles Depends on Multiple Molecular and Physicochemical Mechanisms”, International Journal of Molecular Sciences, 18(12): 2702, (2017). https://doi.org/10.3390/ijms18122702
  • [6] Carmona, E.R., Inostroza-Blancheteau, C., Rubıo, L., Marcos, R., “Genotoxicity of copper oxide nanoparticles in Drosophila melanogaster”, Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 791:1-11, (2015). https://10.1016/j.mrgentox.2015.07.006
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  • [10] Liu, Z., Tang, S., Ai, Z., “Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human hepatoma BEL-7402 cells”, World Journal of Gastroenterology, 9(9): 1968-1971, (2003). https://doi.org/10.3748/wjg.v9.i9.1968
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There are 66 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Biology
Authors

Merve Güneş 0000-0003-3278-0542

Burcin Yalcin 0000-0002-9694-5839

Ayşen Yağmur Kurşun 0000-0003-1657-6808

Ghada Tagorti 0000-0003-4597-8320

Emre Yavuz This is me 0000-0001-9855-7437

Esin Akarsu 0000-0002-1965-5774

Nuray Kaya 0000-0002-3227-6680

Bülent Kaya 0000-0002-0491-9781

Project Number FBA-2018-3285
Early Pub Date September 4, 2023
Publication Date March 1, 2024
Published in Issue Year 2024 Volume: 37 Issue: 1

Cite

APA Güneş, M., Yalcin, B., Kurşun, A. Y., Tagorti, G., et al. (2024). The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells. Gazi University Journal of Science, 37(1), 16-28. https://doi.org/10.35378/gujs.1218829
AMA Güneş M, Yalcin B, Kurşun AY, Tagorti G, Yavuz E, Akarsu E, Kaya N, Kaya B. The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells. Gazi University Journal of Science. March 2024;37(1):16-28. doi:10.35378/gujs.1218829
Chicago Güneş, Merve, Burcin Yalcin, Ayşen Yağmur Kurşun, Ghada Tagorti, Emre Yavuz, Esin Akarsu, Nuray Kaya, and Bülent Kaya. “The Effects of Rod and Round-Like Nanohydroxyapatites on Allium Cepa Root Meristem Cells”. Gazi University Journal of Science 37, no. 1 (March 2024): 16-28. https://doi.org/10.35378/gujs.1218829.
EndNote Güneş M, Yalcin B, Kurşun AY, Tagorti G, Yavuz E, Akarsu E, Kaya N, Kaya B (March 1, 2024) The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells. Gazi University Journal of Science 37 1 16–28.
IEEE M. Güneş, B. Yalcin, A. Y. Kurşun, G. Tagorti, E. Yavuz, E. Akarsu, N. Kaya, and B. Kaya, “The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells”, Gazi University Journal of Science, vol. 37, no. 1, pp. 16–28, 2024, doi: 10.35378/gujs.1218829.
ISNAD Güneş, Merve et al. “The Effects of Rod and Round-Like Nanohydroxyapatites on Allium Cepa Root Meristem Cells”. Gazi University Journal of Science 37/1 (March 2024), 16-28. https://doi.org/10.35378/gujs.1218829.
JAMA Güneş M, Yalcin B, Kurşun AY, Tagorti G, Yavuz E, Akarsu E, Kaya N, Kaya B. The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells. Gazi University Journal of Science. 2024;37:16–28.
MLA Güneş, Merve et al. “The Effects of Rod and Round-Like Nanohydroxyapatites on Allium Cepa Root Meristem Cells”. Gazi University Journal of Science, vol. 37, no. 1, 2024, pp. 16-28, doi:10.35378/gujs.1218829.
Vancouver Güneş M, Yalcin B, Kurşun AY, Tagorti G, Yavuz E, Akarsu E, Kaya N, Kaya B. The Effects of Rod and Round-Like Nanohydroxyapatites on Allium cepa Root Meristem Cells. Gazi University Journal of Science. 2024;37(1):16-28.