Research Article
BibTex RIS Cite

Assessment of Morphological Traits and Resistance to Tomato Spotted Wilt Virus (TSWV) in Hybrids Derived from Various Indigenous Pepper Varieties

Year 2024, Volume: 28 Issue: 3, 508 - 523, 28.09.2024

Gökhan Kartal , Hakan Kartal , Naif Gebeloğlu

https://doi.org/10.29050/harranziraat.1434593

Abstract

Tokat Pepper (Capsicum annuum L.) is a popular variety of pepper that has been cultivated and consumed in Tokat for many years. The production of this cultivar is hindered by various biotic stress factors, one of which is the tomato spotted wilt virus (TSWV) that causes significant economic losses in pepper farming. In this research, TSWV-resistant hybrid plants were cultivated using local pepper lines, and the yield, fruit characteristics, and resistance levels of these hybrids were analysed. Three different strains of 'Tokat Pepper', which had undergone self-fertilisation to reach the fifth generation, were interbred with two donor plants that were resistant to TSWV and carried the TSW gene. The hybrid plants were cultivated in soilless farming conditions and TSWV was transmitted by both mechanical means and the use of floral thrips (Frankliniella occidentalis). While the marketable yields of the parent plants ranged from 2.260 kg per plant to 2.727 kg per plant, the yields of the hybrid genotypes varied from 1.917 kg per plant to 2.527 kg per plant. Marketable yields of the hybrid genotypes were lower than those of the parents. The hybrid plants exhibited heterozygous resistance to TSWV. However, it was observed that resistance in hybrid plants was compromised when the air temperature surpassed 32°C in the screenhouse. The study revealed that fruit shape of the hybrid plants was either 3 or 4 lobed, and fruit colour ranged from light green to dark green. In conclusion, the TSWV resistance gene TSW has been effectively transferred to hybrid plants that were bred from native pepper varieties. Although hybrid plants showed lower yield, there was no visible effect on the fruit's appearance. Resistance was observed to break down at high temperatures, as it has been frequently reported in literature that the TSW gene may exhibit such problems. The study discovered that hybrid varieties of 'Tokat Pepper' can be developed to resist TSWV.

Keywords

TSWV Tokat Biberi Resistance Quality Yield

Project Number

2020/93

References

  • Adamu, U., & Ado, S. G. (1988). Genotypic variability in fruit characteristics of pepper. (Capsicum spp.).
  • Ahmed, N., & Muzafar, H. (2000). Heterosis studies for fruit yield and some economic characters in sweet pepper (Capsicum annuum L.). Capsicum & Eggplant Newsletter, (19), 74-77.
  • Akıncı, S., Çağlar, G., Akıncı İ. E., Doğar, N., & Aras, V. (1998). Bazı yabancı çeşitlerin kurutmalık kırmızı biber üretimine uygunluklarının belirlenmesi. 2. Sebze Tarımı Sempozyumu 28- 30 Eylül. Tokat.
  • Alegbejo, M. D., & Orakwue, F. C. (2002). Characteristics of some pepper cultivars commonly grown in Nigeria. Capsicum Eggplant Newsletter No: 21:2-24.
  • Anonymous (2019). Food and Agriculture Organization of the United Nations (FAO). FAOSTAT,http://www.fao.org/faostat/en/#data/QC.(ErişimTarihi:11.06.2019).
  • Avila, Y., Stavisky, J., Hague, S., Funderburk, J., Reitz, S., & Momol, T. (2006). Evaluation of frankliniella bispinosa (Thysanoptera: Thripidae) as a vector of the tomato spotted wilt virus in pepper. Florida Entomologist, 89(2), 204-207.
  • Aybak, H. Ç. (2002). Biber yetiştiriciliği. Hasad yayıncılık, İstanbul.
  • Ballester, J., & De Vicente, M. C. (1998). Determination of F1 hybrid seed purity in pepper using PCRbased markers. Euphytica 103:223–226.
  • Barroso, P. A., Rêgo E. R., Rêgo M. M., Nascimento K. S., Nascimento N. F. F., Nascimento M. F., Soares W. S., Ferreira K. T. C., & Otoni W. C. (2012). Analysis of segregating generation for components of seedling and plant height of pepper (Capsicum annuum L.) for medicinal and ornamental purposes. Acta Hortic 953:269–275.
  • Bıles, C. L., Martıny, R. D., & Wilson, H. D. (1989). Isoenzymes and general proteins from various watermelon cultivars and tissue types. Hort. Science. 24:810 – 812.
  • Black, L. L. (1991). Year- Round Vegetable Production Systems. Effects of grafting and shelters on sweet peppers grown in the hot-wet season. AVRDC Progress Report 2002. P.61.
  • Black, L. L., Hobbs, H. A., & Gatti, J. M. (1991). Tomato spotted wilt virus resistance in Capsicum chinense ‘PI 152225’ and ‘PI 159236’. Plant Dis 75: 863.
  • Boiteux, L. S., Nagata, T., Dutra, W. P., & Fonseca, M. E. N. (1993). Sources of resistance to tomato spotted wilt virus (TSWV) i n cultivated and wild s pecies o f Capsicum. Euphytica, 6 7:89-94.
  • Boiteux, L. S., & De Avila, A. C. (1994). Inheritance of a resistance specific to tomato spotted wilt tospovirus in Capsicum chinense ‘PI 159236’. Euphytica, 75(1), 139- 142.
  • Boiteux, L. S., (1995). Allelic relationships between genes for resistance to tomato spotted wilt tospovirus in Capsicum chinense. Theor. Appl. Genet. 90: 1 46–149.
  • Bosland P. W., & Votava E. J. (2003). Peppers: vegetable and spice capsicums. CABI, New York, 204p.
  • Bozokalfa, K., Eşiyok, D., & Turhan, K. (2009). Patterns of phenotypic variation in a germplasm collection of pepper (Capsicum annuum L.) from Turkey. Spanish Journal of Agricultural, 7 (1):83-95. Capsicum and Eggplant Newsletter. 7: 46.
  • Cheng, S. S., Green, S. K., Griggs, T. D., & McLean, B. T. (1989). The use of Capsicum chinense as sweet pepper cultivars and sources for gene transfer. In: tomato and pepper production in the tropics. Proceedings of the international symposium on integrated management practices, Tainan, Taiwan, 2 1-26 M arch 1988.
  • Cherian, E. V., & Indira, P. (2003). Variability in Capsicum chinense Jacq. germplasm. capsicum and eggplant newsletter. 22:39-43.
  • Chiemsombat, P., & Adkins, S. (2006). Tospoviruses In: characterization, diagnosis and management of plant viruses (Rao, G.P., Lava Kumar, P. And Holguín-Peña, R.J., eds), pp. 1–37. Houston, TX: studium press.
  • Crossa, J., Campos, G. D. L., Pérez, P., Gianola, D., Burgueño, J., Araus, J. L., & Braun, H. J. (2010). Prediction of genetic values of quantitative traits in plant breeding using pedigree and molecular markers. Genetics, 186(2), 713-724.
  • Çelik, İ., Özalp, R., Çelik, N., Polat, İ., & Görkem, S. Ü. L. Ü. (2018). Domates lekeli solgunluk virüsü (TSWV)’ne dayanıklı sivri biber hatlarının geliştirilmesi. Derim, 35(1), 27-36.
  • Çetin, A. N. (2023). Bazı kapya biber genotiplerinin TSWV (Domates Lekeli Solgunluk Virüsü) ve TMV (Tütün Mozaik Virüs)’ye dayanım düzeylerinin belirlenmesi ve heterotik gruplarının oluşturulması.
  • De Avila, A. C., Haan, P. D., Kitajima, E. W., Kormelink, R., Resende, R. D. O., Goldbach, R. W., & Peters, D. (1992). Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from Impatiens sp. in the Netherlands. Journal of Phytopathology, 134(2), 133-151.
  • De Ronde, D., Pasquier, A., Ying, S., Butterbach, P., Lohuis, D., & Kormelink, R. (2014). Analysis of Tomato spotted wilt virus NSs protein indicates the importance of the N-terminal domain for avirulence and RNA silencing suppression. Molecular Plant Pathology, 15, 185–195.
  • Duman, İ., & Düzyaman, E. (2004). Türkiye’de yetiştirilen bazı önemli biber genotiplerinin morfolojik varyabilitesi üzerine bir araştırma. Ege Ü.Z.F. Dergisi. 41 (3):55-56.
  • El Tahir, I. M. (1994). Collection and Characterization of Hot Pepper Germplasm in Sudan. Horticultural Germplasm Unit, Agricultural Research Corporation. Wad Medani, Sudan. Capsicum and Eggplant Newsletter. 13:36-39. Emmanuel-Ikpeme, C., Henry, P., & Okiri, O. A. (2014). Comparative evaluation of the nutritional, phytochemical and microbiological quality of three pepper varieties. Journal of food and nutrition sciences, 2(3), 74-80. Eshbaugh, W.H. (1980). The taxonomy of the genus Capsicum (Solanaceae). Phytologia 47:153–165.
  • Fao, (2021). World Pepper Production List, https://www.fao.org/faostat/en/#data/QCL: [Erişim Tarihi: 26.06. 2024].
  • Fehr, W. R. (1987). Principle of Cultivar Development. Theory and Technique. Vol. I. MacMillan Pub. Co. New York. 536p.
  • Fery, R. L., & Dukes, P. D. (2005). Potential for utilization of pepper germplasm with a variable reaction to Sclerotium rolfsii Sacc. to develop southern blight-resistant pepper (Capsicum annuum L.) cultivars. Plant Genetic Resources, 3(3), 326-330.
  • Fidan, H., & Nuray, S. A. R. I. (2019). Domateste Tomato spotted wilt virüs’ üne karşı dayanıklılığı kıran izolatının fenotipik karakterizasyonu. Mediterranean Agricultural Sciences, 32(3), 307-314.
  • Florini, D. A., & Zitter, T. A. (1987). Cucumber mosaic virus (CMV) in peppers (Capsicum annuum L.) in New York and associated yield losses. Phytopathology, 77: 652.
  • Geleta, L. F., & Labuschagne, M. T. (2004). Hybrid performance for yield and other characteristics in peppers (Capsicum annuum L.). The Journal of Agricultural Science, 142(4), 411-419.
  • Geleta, L. F., Labuschagne, M. T., & Viljoen, C. D. (2004). Relationship between heterosis and genetic distance based on morphological traits and AFLP markers in pepper. Plant Breeding, 123(5), 467-473.
  • German, T.L., Ullman, D.E., & Moyer, J.W. (1992). Tospoviruses: diagnosis, molecular biology, and vector relationships. Annu Rev Phytopathol 30: 315–348 ejpp835.tex; 3/07/1998; 10:01; p.9.
  • Gil-Ortega, R., & Luis, M. (1994). Should ‘PI 152225’ (Capsicum chinense) resistance to tomato spotted wilt virus (TSWV) be used in breeding programs? Capsicum Newslet 13: 88–89.
  • Gitaitis, R. D., Dowler, C. C., & Chalfant, R. B. (1998). Epidemiology of tomato spotted wilt in pepper and tomato in southern Georgia. Plant Disease, 82 (7), 752-756.
  • Gnayem, N. 1995. Epidemiological and biological aspects of tomato spotted wilt virus-TSWV (Doctoral dissertation, M. Sc. Thesis Submitted to the Faculty of Agriculture of Hebrew University of Jeruselam, Rehavot, Israel).
  • Green, S. K., & Kim, J. S. (1991). Source of resistance to viruses of pepper (Capsicum spp.): A catalog. Technical Bulletin, 20, AVRDC, 64.
  • Greenleaf W. H. (1986). Pepper breeding. In: Basset MJ (ed) Breeding vegetable crops.
  • Groves, R. L., Walgenbach, J. F., Moyer, J. W., & Kennedy, G. G. (2002). The role of weed hosts and tobacco thrips, Frankliniella fusca, in the epidemiology of Tomato spotted wilt virus. Plant Disease, 86(6), 573-582.
  • Gvozdenovic, D. J. (2002). Characterıstıcs of The Assortment of Novı Sad Peppers. Acta Horticulture 579:177-179.
  • Hanssen, I. M., Lapidot, M., & Thomma, B. P. H. J. (2010). Emerging viral diseases of tomato crops. Molecular Plant-Microbe Interactions, 23:539–548.
  • İlbi, H. (2003). RAPD markers assisted varietal identification and genetic purity test in pepper, Capsicum annuum. Sci Hortic 97:211–218.
  • Irikova, T., Grozeva, S., & Rodeva, V. (2011). Anther culture in pepper (Capsicum annuum L.) in vitro. Acta Physiologiae Plantarum, 33(5), 1559-1570.
  • Jahn, M., Paran, I., Hoffmann, K., Radwanski, E. R., Livingstone, K. D., Grube, R. C., & Moyer, J. (2000). Genetic mapping of the TSW locus for resistance to the Tospovirus Tomato spotted wilt virus in Capsicum spp. and its relationship to the Sw-5 gene for resistance to the same pathogen in tomato. Molecular Plant-Microbe Interactions, 13(6), 673-682.
  • Karaağaç, O., & Balkaya, A. (2010). Bafra Kırmızı Biber Populasyonlarının [Capsicum annuum L. var. conoides (Mill.) Irish] Tanımlanması ve Mevcut Varyasyonun Değerlendirilmesi. Anadolu Tarım Bilimleri Dergisi, 25(1), 10-20.
  • Karagül, S., Keleş, D., & Demirtaş, B. (2005). Güneydoğu Anadolu Bölgesinde Biber (Capsicum annum) Yetiştiriciliğinin Problemleri ve Çözüm Önerileri. GAP IV. Tarım Kongresi, 21-23Eylül, s. 154–161. Şanlıurfa.
  • Keleş, D. (2007). Farklı biber tiplerinin karakterizasyonu ve düşük sıcaklığa tolerans. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi (Basılmamış). 212 s. Adana.
  • Keleş, D., Rastgeldi, U., Karaipçin, Z., Karagül, S., Soylu, M.K., Çömlekçioğlu, N., & Büyükalaca, S. (2016). Seleksiyon Yoluyla Şanlıurfa Biber Islahı. Alata Bahçe Kültürleri Araştırma Enstitüsü Müdürlüğü Erdemli, Mersin. Ala tarım 2016, 15 (1): 39-44.
  • Kenyon, L., Kumar, S., Tsai, W. S., & Hughes, J. D. (2014). Virus diseases of peppers (Capsicum spp.) and their control. Advances in Virus Research, 90:297‐354. doi:10.1016/B978-0-12-801246-8.00006-8.
  • Kim, M., Jang, I. C., Kim, J. A., Park, E. J., Yoon, M., & Lee, Y. (2008). Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture. Plant cell reports, 27(3), 425-434.
  • Lee, J. M., (1994). Cultivation of grafted vegetables. I. current status grafting methods and benefits. Hortscience, 29(4):235-239.
  • Lefebvre, V., (2004). Molecular markers for genetics and breeding: development and use in pepper (Capsicum spp.). In Molecular marker systems in plant breeding and crop improvement (pp. 189-214). Springer, Berlin, Heidelberg.
  • Livneh, O., Vardi, E., Stram, Y., Edelbaum, O., & Sela, I. (1992). The conversion of a RFLP assay into PCR for the determination of purity in a hybrid pepper cultivar. Euphytica 62:97–102.
  • Marame, F., Dessalegne, L., Fininsa, C., & Sigvald, R. (2009). Heterosis and heritability in crosses among Asian and Ethiopian parents of hot pepper genotypes. Euphytica, 168(2), 235-247.
  • Margaria, P., Ciuffo, M., Pacifico, D., & Turina, M. (2007). Evidence that the nonstructural protein of Tomato spotted wilt virus is the avirulence determinant in the interaction with resistant pepper carrying the TSW gene. Molecular Plant-Microbe Interactions, 20:547–558.
  • Mohamed, M. B. (1984). Evaluation of Sweet Pepper Cultivars in the Sudan. Acta Horticulturae 143, Tropical Horticulture VIII. 189- 193.
  • Moury, B., & Verdin E. (2012). Viruses of pepper crops in the Mediterranean basin: a remarkable stasis. Advances in Virus Research, 84, 127–162.
  • Moury, B., Palloix, A., Gebre-Selassie, K., & Marchoux, G. (1997). Hypersensitive resistance to Tomato spotted wilt virus in three Capsicum chinense accessions is controlled by a single gene and is overcome by virulent strains. Euphytica, 94:45– 52.
  • Moury, B., Pflieger, S., Blattes, A., Lefebvre, V., & Palloıx, A. (2000). A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper. Genome, 43 (1): 137–142.
  • Moury, B., Selassie, K. G., Marchoux, G., Daubèze, A. M., & Palloix, A. (1998). High temperature effects on hypersensitive resistance to tomato spotted wilt tospovirus (TSWV) in pepper (Capsicum chinense J.). European Journal of Plant Pathology, 104(5), 489-498.
  • Mutlu, S., Haytaoğlu, A. Kır, A., & İçer, B. (2009). Ulusal gen bankası biber (Capsicum annum L.) materyalinde morfolojik karakterizasyon. Anadolu, J. of AARI. 1 (1):63- 91.
  • Nuez, F., Diez, M. J., Roselló, S., Lacasa, A., Jordá, C., Martin, M., & Costa, J. (1994). Genetic resistance to TSWV (tomato spotted wilt virus) in Capsicum spp. Capsicum Newsl 13: 86–87.
  • Otulaj, A. O., & Makine, M. J. (1994). Assesment of the Vegetative, Reproductive Characters and Fruit Production pattern of Pepper Cultivars (Capsicum spp.). Capsicum Eggplant Newsletter No.13:54-57.
  • Paran, I., Aftergoot, E., & Shifriss C. (1998). Variation in Capsicum annuum revealed by RAPD and AFLP markers. Euphytica 99:167–173.
  • Pardossi, A., Tognoni, F., & Incrocci, L. (2004). Mediterranean greenhouse technology. Chronica horticulturae, 44(2), 28-34.
  • Parrella, G., Gognalons, P., Gebre-Selassie, K., Vovlas, C., & Marchoux, G. (2003). An update of the host range of Tomato spotted wilt virus. Journal of Plant Pathology, 227-264.
  • Rêgo, E. R., Rego, M. M., Finger, F. L., Cruz, C. D., & Casali, V. W. D. (2009). A diallel study of yield components and fruit quality in chilli pepper (Capsicum baccatum). Euphytica 168:275–287.
  • Rêgo, E. R., Rêgo, M. M., Matos, IWF., & Barbosa, L. A. (2011). Morphological and chemical characterization of fruits of Capsicum spp. accessions. Hortic Bras 29:364–371.
  • Rıvero, R. M., Ruız, J. M., & Romero, L. (2003). Role of Grafting in Horticultural Plants Under Stress Conditions. Food, Agriculture and Environment. 1:70 – 74.
  • Roditakis, N. E., Lykouressis, D. P., & Golfinopoulou, N. G. (2001). Color preference, Roggero, P. 2002. Infection of tospoviruses in pepper and control by resistant plants. Biológico, São Paulo, 64(2), 179-181.
  • Roggero, P., Pennazio, S., Masenga, V., & Tavella, L. (2002, December). Resistance to tospoviruses in pepper. In Thrips and tospoviruses: proceedings of the 7th international symposium on Thysanoptera (CD-ROM). A NIC, Canberra (pp. 105-110).
  • Salamon, P., Mitykó, J., Kalo, P., & Szabo, Z. (2016). Symptoms caused by Tomato spotted wilt virus (TSWV) in pepper (Capsicum spp.) and marker assisted selection of TSWV resistant pepper lines for hybrid constructions. In Proceedings XVI, Eucarpia Capsicum and Eggplant Meeting, Kecskemét, Hungary (pp. 12-14).
  • Scholthof, K. B. G., Adkins, S., Czosnek, H., Palukaitis, P., Jacquot, E., Hohn, T., & Hemenway, C. (2011). Top 10 plant viruses in molecular plant pathology. Molecular plant pathology, 12 (9), 938-954.
  • Sharma, V. K., Punetha, S., & Sharma, B. B. (2013). Heterosis studies for earliness, fruit yield and yield attributing traits in bell pepper. African Journal of Agricultural Research, 8(29), 4088-4098.
  • Sharma, V. K., Srivastava, A., & Mangal, M. (2020). Recent Trends in Sweet Pepper Breeding. In Accelerated Plant Breeding, Volume 2 (pp. 417-444). Springer, Cham.
  • Sharman, M., & Persley, D. M. (2006). Field isolates of Tomato spotted wilt virus overcoming resistance in capsicum in Australia. Australasian Plant Pathology, 35(2), 123-128.
  • Shrestha, S. L., Luitel, B. P., & Kang, W. H. (2011). Heterosis and heterobeltiosis studies in sweet pepper (Capsicum annuum L.). Horticulture, Environment, and Biotechnology, 52(3), 278-283. sticky trap catches and distribution of western flower thrips in greenhouse cucumber, sweet pepper and eggplant crops. Southwest. Entomology, 26: 227– 238.
  • Şevik, M. A. (2015). Sebze Üretimini Tehdit Eden Viral Hastalık Etmeni: Domates lekeli solgunluk virüsü (Tomato spotted wilt virus–TSWV). Fen Bilimleri Enst. Dergisi Iğdır Univ. J. Inst. Sci. & Tech, 5(2), 17-23. TÜİK, (2020). https://biruni.tuik.gov.tr/medas/?locale=tr.
  • TÜİK, (2022). Türkiye Biber Üretim Verileri. https://data.tuik.gov.tr/Bulten/Index?p=Bitkisel-Uretim-Istatistikleri-202349535.
  • Ullman, D. E., Sherwood, J. L., & German, T. L. (1997). Thrips as vectors of plant pathogens. Thrips as vectors of plant pathogens., 539-565.
  • Valsikova, M., Strelec, V., & Kopec, K. (1983). Morphological Traits of Sweet Pepper. Capsicum Eggplant Newsletter No.2:73-74.
  • Yoon, J. Y., Green, S. K., Tschanz, A. T., Tsou, S. C. S., & Chang, L. C. (1989). Pepper ımprovement for the tropics, problems and the avrdc approach. Asian Vegetable Research and Development Center, Tomato and Pepper Production in the Tropics, AVRDC, Shanhua, Tainan, 86-98.
  • Zatykó, L. (2006). Pepper (Capsicum annuum L.) breeding methods at the turn of the century. Acta Agronomica Hungarica, 54(2), 179-202.

Bazı yerelbiber tiplerinden elde edilen melezlerin morfolojik özelliklerinin ve Domates Lekeli Solgunluk Virüsüne (TSWV) dayanıklılığının belirlenmesi

Year 2024, Volume: 28 Issue: 3, 508 - 523, 28.09.2024

Gökhan Kartal , Hakan Kartal , Naif Gebeloğlu

https://doi.org/10.29050/harranziraat.1434593

Abstract

Tokat Biberi (Capsicum annuum L.), Tokat'ta uzun yıllardır yetiştirilen ve tüketilen popüler bir biber çeşididir. Bu çeşidin üretimi çeşitli biyotik stres faktörleri tarafından engellenmektedir. Bunlardan biri de biber tarımında önemli ekonomik kayıplara neden olan domates lekeli solgunluk virüsüdür (TSWV). Bu araştırmada, yerel biber hatları kullanılarak TSWV'ye dayanıklı hibrit bitkiler yetiştirilmiş ve bu hibritlerin verim, meyve özellikleri ve dayanıklılık seviyeleri araştırılmıştır. S5 generasyonuna kadar kendilenmiş üç farklı 'Tokat Biberi' hattı, TSWV'ye dayanıklı ve TSW geni taşıyan 2 donör bitki ile melezlenmiştir. Melez bitkiler topraksız tarım koşullarında yetiştirilmiş ve TSWV bitkilere hem mekanik yollarla hem de çiçek tripsleri (Frankliniella occidentalis) kullanılarak bulaştırılmıştır. Ebeveyn bitkilerin pazarlanabilir verimleri bitki başına 2.260 kg ile 2.727 kg arasında değişirken, melez genotiplerin verimleri bitki başına 1.917 kg ile 2.527 kg arasında değişmiştir. Melez genotiplerin pazarlanabilir verimleri ebeveynlerden düşük olmuştur. Melez bitkilerde TSWV'ye karşı heterozigot dayanıklılık belirlenmiştir. Tül serada hava sıcaklığı 32°C'nin üzerine çıktığında melez bitkilerde dayanıklılığın kırıldığı görülmüştür. Çalışmada melez bitkilerin meyve şekli 3 ve 4 loblu, meyve rengi açık yeşil, yeşil ve koyu yeşil olarak gözlenmiştir. Sonuç olarak, yerel biber hatlarından geliştirilen melez bitkilere TSWV dayanıklılık geni TSW başarıyla aktarılmıştır. Melez bitkilerin veriminde düşüş meydana gelirken meyve görünümlerinde bir değişim olmamıştır. Yüksek sıcaklıklarda dayanıklılık kırılmıştır. Literatürde de TSW geninin bazı durumlarda kırıldığı sıklıkla belirtilmektedir. Çalışma sonunda 'Tokat Biberi' popülasyonlarından TSWV'ye dayanıklı hibrit çeşitlerin geliştirilmesi amaçlanmaktadır.

Keywords

TSWV Tokat Biberi Dayanıklılık Kalite Verim

Ethical Statement

Herhangi bir çıkar çatışması olmadığını beyan ederim.

Supporting Institution

Tokat Gaziosmanpaşa Üniversitesi Bilimsel Araştırma Projeleri (BAP) Komisyonu

Project Number

2020/93

Thanks

Çalışmada yapılmış olan analizler Tokat Gaziosmanpaşa Üniversitesi Bahçe Bitkileri Bölümü laboratuvarlarında yürütülmüş olup; katkısı olanlara teşekkür ederiz.

References

  • Adamu, U., & Ado, S. G. (1988). Genotypic variability in fruit characteristics of pepper. (Capsicum spp.).
  • Ahmed, N., & Muzafar, H. (2000). Heterosis studies for fruit yield and some economic characters in sweet pepper (Capsicum annuum L.). Capsicum & Eggplant Newsletter, (19), 74-77.
  • Akıncı, S., Çağlar, G., Akıncı İ. E., Doğar, N., & Aras, V. (1998). Bazı yabancı çeşitlerin kurutmalık kırmızı biber üretimine uygunluklarının belirlenmesi. 2. Sebze Tarımı Sempozyumu 28- 30 Eylül. Tokat.
  • Alegbejo, M. D., & Orakwue, F. C. (2002). Characteristics of some pepper cultivars commonly grown in Nigeria. Capsicum Eggplant Newsletter No: 21:2-24.
  • Anonymous (2019). Food and Agriculture Organization of the United Nations (FAO). FAOSTAT,http://www.fao.org/faostat/en/#data/QC.(ErişimTarihi:11.06.2019).
  • Avila, Y., Stavisky, J., Hague, S., Funderburk, J., Reitz, S., & Momol, T. (2006). Evaluation of frankliniella bispinosa (Thysanoptera: Thripidae) as a vector of the tomato spotted wilt virus in pepper. Florida Entomologist, 89(2), 204-207.
  • Aybak, H. Ç. (2002). Biber yetiştiriciliği. Hasad yayıncılık, İstanbul.
  • Ballester, J., & De Vicente, M. C. (1998). Determination of F1 hybrid seed purity in pepper using PCRbased markers. Euphytica 103:223–226.
  • Barroso, P. A., Rêgo E. R., Rêgo M. M., Nascimento K. S., Nascimento N. F. F., Nascimento M. F., Soares W. S., Ferreira K. T. C., & Otoni W. C. (2012). Analysis of segregating generation for components of seedling and plant height of pepper (Capsicum annuum L.) for medicinal and ornamental purposes. Acta Hortic 953:269–275.
  • Bıles, C. L., Martıny, R. D., & Wilson, H. D. (1989). Isoenzymes and general proteins from various watermelon cultivars and tissue types. Hort. Science. 24:810 – 812.
  • Black, L. L. (1991). Year- Round Vegetable Production Systems. Effects of grafting and shelters on sweet peppers grown in the hot-wet season. AVRDC Progress Report 2002. P.61.
  • Black, L. L., Hobbs, H. A., & Gatti, J. M. (1991). Tomato spotted wilt virus resistance in Capsicum chinense ‘PI 152225’ and ‘PI 159236’. Plant Dis 75: 863.
  • Boiteux, L. S., Nagata, T., Dutra, W. P., & Fonseca, M. E. N. (1993). Sources of resistance to tomato spotted wilt virus (TSWV) i n cultivated and wild s pecies o f Capsicum. Euphytica, 6 7:89-94.
  • Boiteux, L. S., & De Avila, A. C. (1994). Inheritance of a resistance specific to tomato spotted wilt tospovirus in Capsicum chinense ‘PI 159236’. Euphytica, 75(1), 139- 142.
  • Boiteux, L. S., (1995). Allelic relationships between genes for resistance to tomato spotted wilt tospovirus in Capsicum chinense. Theor. Appl. Genet. 90: 1 46–149.
  • Bosland P. W., & Votava E. J. (2003). Peppers: vegetable and spice capsicums. CABI, New York, 204p.
  • Bozokalfa, K., Eşiyok, D., & Turhan, K. (2009). Patterns of phenotypic variation in a germplasm collection of pepper (Capsicum annuum L.) from Turkey. Spanish Journal of Agricultural, 7 (1):83-95. Capsicum and Eggplant Newsletter. 7: 46.
  • Cheng, S. S., Green, S. K., Griggs, T. D., & McLean, B. T. (1989). The use of Capsicum chinense as sweet pepper cultivars and sources for gene transfer. In: tomato and pepper production in the tropics. Proceedings of the international symposium on integrated management practices, Tainan, Taiwan, 2 1-26 M arch 1988.
  • Cherian, E. V., & Indira, P. (2003). Variability in Capsicum chinense Jacq. germplasm. capsicum and eggplant newsletter. 22:39-43.
  • Chiemsombat, P., & Adkins, S. (2006). Tospoviruses In: characterization, diagnosis and management of plant viruses (Rao, G.P., Lava Kumar, P. And Holguín-Peña, R.J., eds), pp. 1–37. Houston, TX: studium press.
  • Crossa, J., Campos, G. D. L., Pérez, P., Gianola, D., Burgueño, J., Araus, J. L., & Braun, H. J. (2010). Prediction of genetic values of quantitative traits in plant breeding using pedigree and molecular markers. Genetics, 186(2), 713-724.
  • Çelik, İ., Özalp, R., Çelik, N., Polat, İ., & Görkem, S. Ü. L. Ü. (2018). Domates lekeli solgunluk virüsü (TSWV)’ne dayanıklı sivri biber hatlarının geliştirilmesi. Derim, 35(1), 27-36.
  • Çetin, A. N. (2023). Bazı kapya biber genotiplerinin TSWV (Domates Lekeli Solgunluk Virüsü) ve TMV (Tütün Mozaik Virüs)’ye dayanım düzeylerinin belirlenmesi ve heterotik gruplarının oluşturulması.
  • De Avila, A. C., Haan, P. D., Kitajima, E. W., Kormelink, R., Resende, R. D. O., Goldbach, R. W., & Peters, D. (1992). Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from Impatiens sp. in the Netherlands. Journal of Phytopathology, 134(2), 133-151.
  • De Ronde, D., Pasquier, A., Ying, S., Butterbach, P., Lohuis, D., & Kormelink, R. (2014). Analysis of Tomato spotted wilt virus NSs protein indicates the importance of the N-terminal domain for avirulence and RNA silencing suppression. Molecular Plant Pathology, 15, 185–195.
  • Duman, İ., & Düzyaman, E. (2004). Türkiye’de yetiştirilen bazı önemli biber genotiplerinin morfolojik varyabilitesi üzerine bir araştırma. Ege Ü.Z.F. Dergisi. 41 (3):55-56.
  • El Tahir, I. M. (1994). Collection and Characterization of Hot Pepper Germplasm in Sudan. Horticultural Germplasm Unit, Agricultural Research Corporation. Wad Medani, Sudan. Capsicum and Eggplant Newsletter. 13:36-39. Emmanuel-Ikpeme, C., Henry, P., & Okiri, O. A. (2014). Comparative evaluation of the nutritional, phytochemical and microbiological quality of three pepper varieties. Journal of food and nutrition sciences, 2(3), 74-80. Eshbaugh, W.H. (1980). The taxonomy of the genus Capsicum (Solanaceae). Phytologia 47:153–165.
  • Fao, (2021). World Pepper Production List, https://www.fao.org/faostat/en/#data/QCL: [Erişim Tarihi: 26.06. 2024].
  • Fehr, W. R. (1987). Principle of Cultivar Development. Theory and Technique. Vol. I. MacMillan Pub. Co. New York. 536p.
  • Fery, R. L., & Dukes, P. D. (2005). Potential for utilization of pepper germplasm with a variable reaction to Sclerotium rolfsii Sacc. to develop southern blight-resistant pepper (Capsicum annuum L.) cultivars. Plant Genetic Resources, 3(3), 326-330.
  • Fidan, H., & Nuray, S. A. R. I. (2019). Domateste Tomato spotted wilt virüs’ üne karşı dayanıklılığı kıran izolatının fenotipik karakterizasyonu. Mediterranean Agricultural Sciences, 32(3), 307-314.
  • Florini, D. A., & Zitter, T. A. (1987). Cucumber mosaic virus (CMV) in peppers (Capsicum annuum L.) in New York and associated yield losses. Phytopathology, 77: 652.
  • Geleta, L. F., & Labuschagne, M. T. (2004). Hybrid performance for yield and other characteristics in peppers (Capsicum annuum L.). The Journal of Agricultural Science, 142(4), 411-419.
  • Geleta, L. F., Labuschagne, M. T., & Viljoen, C. D. (2004). Relationship between heterosis and genetic distance based on morphological traits and AFLP markers in pepper. Plant Breeding, 123(5), 467-473.
  • German, T.L., Ullman, D.E., & Moyer, J.W. (1992). Tospoviruses: diagnosis, molecular biology, and vector relationships. Annu Rev Phytopathol 30: 315–348 ejpp835.tex; 3/07/1998; 10:01; p.9.
  • Gil-Ortega, R., & Luis, M. (1994). Should ‘PI 152225’ (Capsicum chinense) resistance to tomato spotted wilt virus (TSWV) be used in breeding programs? Capsicum Newslet 13: 88–89.
  • Gitaitis, R. D., Dowler, C. C., & Chalfant, R. B. (1998). Epidemiology of tomato spotted wilt in pepper and tomato in southern Georgia. Plant Disease, 82 (7), 752-756.
  • Gnayem, N. 1995. Epidemiological and biological aspects of tomato spotted wilt virus-TSWV (Doctoral dissertation, M. Sc. Thesis Submitted to the Faculty of Agriculture of Hebrew University of Jeruselam, Rehavot, Israel).
  • Green, S. K., & Kim, J. S. (1991). Source of resistance to viruses of pepper (Capsicum spp.): A catalog. Technical Bulletin, 20, AVRDC, 64.
  • Greenleaf W. H. (1986). Pepper breeding. In: Basset MJ (ed) Breeding vegetable crops.
  • Groves, R. L., Walgenbach, J. F., Moyer, J. W., & Kennedy, G. G. (2002). The role of weed hosts and tobacco thrips, Frankliniella fusca, in the epidemiology of Tomato spotted wilt virus. Plant Disease, 86(6), 573-582.
  • Gvozdenovic, D. J. (2002). Characterıstıcs of The Assortment of Novı Sad Peppers. Acta Horticulture 579:177-179.
  • Hanssen, I. M., Lapidot, M., & Thomma, B. P. H. J. (2010). Emerging viral diseases of tomato crops. Molecular Plant-Microbe Interactions, 23:539–548.
  • İlbi, H. (2003). RAPD markers assisted varietal identification and genetic purity test in pepper, Capsicum annuum. Sci Hortic 97:211–218.
  • Irikova, T., Grozeva, S., & Rodeva, V. (2011). Anther culture in pepper (Capsicum annuum L.) in vitro. Acta Physiologiae Plantarum, 33(5), 1559-1570.
  • Jahn, M., Paran, I., Hoffmann, K., Radwanski, E. R., Livingstone, K. D., Grube, R. C., & Moyer, J. (2000). Genetic mapping of the TSW locus for resistance to the Tospovirus Tomato spotted wilt virus in Capsicum spp. and its relationship to the Sw-5 gene for resistance to the same pathogen in tomato. Molecular Plant-Microbe Interactions, 13(6), 673-682.
  • Karaağaç, O., & Balkaya, A. (2010). Bafra Kırmızı Biber Populasyonlarının [Capsicum annuum L. var. conoides (Mill.) Irish] Tanımlanması ve Mevcut Varyasyonun Değerlendirilmesi. Anadolu Tarım Bilimleri Dergisi, 25(1), 10-20.
  • Karagül, S., Keleş, D., & Demirtaş, B. (2005). Güneydoğu Anadolu Bölgesinde Biber (Capsicum annum) Yetiştiriciliğinin Problemleri ve Çözüm Önerileri. GAP IV. Tarım Kongresi, 21-23Eylül, s. 154–161. Şanlıurfa.
  • Keleş, D. (2007). Farklı biber tiplerinin karakterizasyonu ve düşük sıcaklığa tolerans. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi (Basılmamış). 212 s. Adana.
  • Keleş, D., Rastgeldi, U., Karaipçin, Z., Karagül, S., Soylu, M.K., Çömlekçioğlu, N., & Büyükalaca, S. (2016). Seleksiyon Yoluyla Şanlıurfa Biber Islahı. Alata Bahçe Kültürleri Araştırma Enstitüsü Müdürlüğü Erdemli, Mersin. Ala tarım 2016, 15 (1): 39-44.
  • Kenyon, L., Kumar, S., Tsai, W. S., & Hughes, J. D. (2014). Virus diseases of peppers (Capsicum spp.) and their control. Advances in Virus Research, 90:297‐354. doi:10.1016/B978-0-12-801246-8.00006-8.
  • Kim, M., Jang, I. C., Kim, J. A., Park, E. J., Yoon, M., & Lee, Y. (2008). Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture. Plant cell reports, 27(3), 425-434.
  • Lee, J. M., (1994). Cultivation of grafted vegetables. I. current status grafting methods and benefits. Hortscience, 29(4):235-239.
  • Lefebvre, V., (2004). Molecular markers for genetics and breeding: development and use in pepper (Capsicum spp.). In Molecular marker systems in plant breeding and crop improvement (pp. 189-214). Springer, Berlin, Heidelberg.
  • Livneh, O., Vardi, E., Stram, Y., Edelbaum, O., & Sela, I. (1992). The conversion of a RFLP assay into PCR for the determination of purity in a hybrid pepper cultivar. Euphytica 62:97–102.
  • Marame, F., Dessalegne, L., Fininsa, C., & Sigvald, R. (2009). Heterosis and heritability in crosses among Asian and Ethiopian parents of hot pepper genotypes. Euphytica, 168(2), 235-247.
  • Margaria, P., Ciuffo, M., Pacifico, D., & Turina, M. (2007). Evidence that the nonstructural protein of Tomato spotted wilt virus is the avirulence determinant in the interaction with resistant pepper carrying the TSW gene. Molecular Plant-Microbe Interactions, 20:547–558.
  • Mohamed, M. B. (1984). Evaluation of Sweet Pepper Cultivars in the Sudan. Acta Horticulturae 143, Tropical Horticulture VIII. 189- 193.
  • Moury, B., & Verdin E. (2012). Viruses of pepper crops in the Mediterranean basin: a remarkable stasis. Advances in Virus Research, 84, 127–162.
  • Moury, B., Palloix, A., Gebre-Selassie, K., & Marchoux, G. (1997). Hypersensitive resistance to Tomato spotted wilt virus in three Capsicum chinense accessions is controlled by a single gene and is overcome by virulent strains. Euphytica, 94:45– 52.
  • Moury, B., Pflieger, S., Blattes, A., Lefebvre, V., & Palloıx, A. (2000). A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper. Genome, 43 (1): 137–142.
  • Moury, B., Selassie, K. G., Marchoux, G., Daubèze, A. M., & Palloix, A. (1998). High temperature effects on hypersensitive resistance to tomato spotted wilt tospovirus (TSWV) in pepper (Capsicum chinense J.). European Journal of Plant Pathology, 104(5), 489-498.
  • Mutlu, S., Haytaoğlu, A. Kır, A., & İçer, B. (2009). Ulusal gen bankası biber (Capsicum annum L.) materyalinde morfolojik karakterizasyon. Anadolu, J. of AARI. 1 (1):63- 91.
  • Nuez, F., Diez, M. J., Roselló, S., Lacasa, A., Jordá, C., Martin, M., & Costa, J. (1994). Genetic resistance to TSWV (tomato spotted wilt virus) in Capsicum spp. Capsicum Newsl 13: 86–87.
  • Otulaj, A. O., & Makine, M. J. (1994). Assesment of the Vegetative, Reproductive Characters and Fruit Production pattern of Pepper Cultivars (Capsicum spp.). Capsicum Eggplant Newsletter No.13:54-57.
  • Paran, I., Aftergoot, E., & Shifriss C. (1998). Variation in Capsicum annuum revealed by RAPD and AFLP markers. Euphytica 99:167–173.
  • Pardossi, A., Tognoni, F., & Incrocci, L. (2004). Mediterranean greenhouse technology. Chronica horticulturae, 44(2), 28-34.
  • Parrella, G., Gognalons, P., Gebre-Selassie, K., Vovlas, C., & Marchoux, G. (2003). An update of the host range of Tomato spotted wilt virus. Journal of Plant Pathology, 227-264.
  • Rêgo, E. R., Rego, M. M., Finger, F. L., Cruz, C. D., & Casali, V. W. D. (2009). A diallel study of yield components and fruit quality in chilli pepper (Capsicum baccatum). Euphytica 168:275–287.
  • Rêgo, E. R., Rêgo, M. M., Matos, IWF., & Barbosa, L. A. (2011). Morphological and chemical characterization of fruits of Capsicum spp. accessions. Hortic Bras 29:364–371.
  • Rıvero, R. M., Ruız, J. M., & Romero, L. (2003). Role of Grafting in Horticultural Plants Under Stress Conditions. Food, Agriculture and Environment. 1:70 – 74.
  • Roditakis, N. E., Lykouressis, D. P., & Golfinopoulou, N. G. (2001). Color preference, Roggero, P. 2002. Infection of tospoviruses in pepper and control by resistant plants. Biológico, São Paulo, 64(2), 179-181.
  • Roggero, P., Pennazio, S., Masenga, V., & Tavella, L. (2002, December). Resistance to tospoviruses in pepper. In Thrips and tospoviruses: proceedings of the 7th international symposium on Thysanoptera (CD-ROM). A NIC, Canberra (pp. 105-110).
  • Salamon, P., Mitykó, J., Kalo, P., & Szabo, Z. (2016). Symptoms caused by Tomato spotted wilt virus (TSWV) in pepper (Capsicum spp.) and marker assisted selection of TSWV resistant pepper lines for hybrid constructions. In Proceedings XVI, Eucarpia Capsicum and Eggplant Meeting, Kecskemét, Hungary (pp. 12-14).
  • Scholthof, K. B. G., Adkins, S., Czosnek, H., Palukaitis, P., Jacquot, E., Hohn, T., & Hemenway, C. (2011). Top 10 plant viruses in molecular plant pathology. Molecular plant pathology, 12 (9), 938-954.
  • Sharma, V. K., Punetha, S., & Sharma, B. B. (2013). Heterosis studies for earliness, fruit yield and yield attributing traits in bell pepper. African Journal of Agricultural Research, 8(29), 4088-4098.
  • Sharma, V. K., Srivastava, A., & Mangal, M. (2020). Recent Trends in Sweet Pepper Breeding. In Accelerated Plant Breeding, Volume 2 (pp. 417-444). Springer, Cham.
  • Sharman, M., & Persley, D. M. (2006). Field isolates of Tomato spotted wilt virus overcoming resistance in capsicum in Australia. Australasian Plant Pathology, 35(2), 123-128.
  • Shrestha, S. L., Luitel, B. P., & Kang, W. H. (2011). Heterosis and heterobeltiosis studies in sweet pepper (Capsicum annuum L.). Horticulture, Environment, and Biotechnology, 52(3), 278-283. sticky trap catches and distribution of western flower thrips in greenhouse cucumber, sweet pepper and eggplant crops. Southwest. Entomology, 26: 227– 238.
  • Şevik, M. A. (2015). Sebze Üretimini Tehdit Eden Viral Hastalık Etmeni: Domates lekeli solgunluk virüsü (Tomato spotted wilt virus–TSWV). Fen Bilimleri Enst. Dergisi Iğdır Univ. J. Inst. Sci. & Tech, 5(2), 17-23. TÜİK, (2020). https://biruni.tuik.gov.tr/medas/?locale=tr.
  • TÜİK, (2022). Türkiye Biber Üretim Verileri. https://data.tuik.gov.tr/Bulten/Index?p=Bitkisel-Uretim-Istatistikleri-202349535.
  • Ullman, D. E., Sherwood, J. L., & German, T. L. (1997). Thrips as vectors of plant pathogens. Thrips as vectors of plant pathogens., 539-565.
  • Valsikova, M., Strelec, V., & Kopec, K. (1983). Morphological Traits of Sweet Pepper. Capsicum Eggplant Newsletter No.2:73-74.
  • Yoon, J. Y., Green, S. K., Tschanz, A. T., Tsou, S. C. S., & Chang, L. C. (1989). Pepper ımprovement for the tropics, problems and the avrdc approach. Asian Vegetable Research and Development Center, Tomato and Pepper Production in the Tropics, AVRDC, Shanhua, Tainan, 86-98.
  • Zatykó, L. (2006). Pepper (Capsicum annuum L.) breeding methods at the turn of the century. Acta Agronomica Hungarica, 54(2), 179-202.
There are 85 citations in total.

Details

Primary Language Turkish
Subjects Vegetable Growing and Treatment
Journal Section Araştırma Makaleleri
Authors

Gökhan Kartal 0000-0003-4026-0360

Hakan Kartal 0000-0002-3870-1588

Naif Gebeloğlu 0000-0003-2495-7088

Project Number 2020/93
Early Pub Date September 28, 2024
Publication Date September 28, 2024
Submission Date February 9, 2024
Acceptance Date July 24, 2024
Published in Issue Year 2024 Volume: 28 Issue: 3

Cite

APA Kartal, G., Kartal, H., & Gebeloğlu, N. (2024). Bazı yerelbiber tiplerinden elde edilen melezlerin morfolojik özelliklerinin ve Domates Lekeli Solgunluk Virüsüne (TSWV) dayanıklılığının belirlenmesi. Harran Tarım Ve Gıda Bilimleri Dergisi, 28(3), 508-523. https://doi.org/10.29050/harranziraat.1434593
 Download Cover Image

Indexing and Abstracting 

13435  19617 13436 13440 13441 13442 13443

13445 13447 13449 13464 13466


10749  Harran Journal of Agricultural and Food Science is licensed under Creative Commons 4.0 International License.