Journal of Animal Production

1301-9597 2645-9043

Ege Zootekni Derneği

10.29185/hayuretim.1268591

Sheep and Goat Breeding and Treatment Animal Science, Genetics and Biostatistics

Küçükbaş Hayvan Yetiştirme ve Islahı Zootekni, Genetik ve Biyoistatistik

Genetic Variations in HSP90AA1 Gene Region in Pırlak Sheep Breed

Pırlak koyunlarında HSP90AA1 gen bölgesindeki genetik varyasyonlar

https://orcid.org/0000-0003-1824-5391

Yurdagül

Kadriye Gül

AKDENIZ UNIVERSITY

https://orcid.org/0000-0003-4542-3499

Atay

Sude

AKDENIZ UNIVERSITY

https://orcid.org/0000-0002-6217-5223

Bilginer

Ümit

AKDENIZ UNIVERSITY

https://orcid.org/0000-0002-2413-1713

Karslı

Taki

ESKISEHIR OSMANGAZI UNIVERSITY

https://orcid.org/0000-0003-4507-7426

Demir

Eymen

AKDENIZ UNIVERSITY

10 30 2023

64 1 12 16 03 21 2023 07 02 2023

1974

Journal of Animal Production

Objective: This study aimed to reveal variations in HSP90AA1 gene region in Pırlak sheep breed.Material and Methods: A total of 100 animals randomly chosen from representative herds raised in Antalya province were genotyped by Allele-Specific Polymerase Chain Reaction (AS-PCR) technique and genotypes were validated via agarose gel electrophoresis.Results: HSP90AA1 region was found to be polymorphic in which two alleles (C and G) and three possible genotypes (CC, CG and GG) were detected. The frequencies of C and G alleles were 0.39 and 0.61, respectively, whereas genotype frequency ranged from 0.183 (CC) to 0.413 (GG). The frequency of CG genotype was calculated as 0.404. Conservation of the genetic variations at HSP90AA1 region will be required for planning selection programs against heat stress in the future.Conclusion: HSP90AA1 and similar genes may offer new opportunities to reduce heat stress caused by global climate change in the near future. Additionally, more genetic studies for the other genomic regions related to environmental stressors should be conducted in Pırlak breed in order to shape conservation studies.

Amaç: Bu çalışmanın amacı Pırlak koyun ırkında HSP90AA1 geni varyasyonlarının incelenmesidir.Materyal ve Metot: Antalya ilinde ırkı temsil eden sürülerden rastgele seçilen toplam 100 akraba olmayan hayvan Allel-Spesifik Polimeraz Zincir Reaksiyonu (AS-PZR) tekniğiyle genotiplendirilmiş ve genotipler agaroz jel elektorforeziyle tespit edilmiştir.Bulgular: İki allel (C ve G) ve olası üç genotipin (CC, CG ve GG) tespit edildiği HSP90AA1 bölgesinin polimorfik olduğu belirlenmiştir. C ve G allel frekansı sırasıyla 0.39 ve 0.61 idi, genotip frekansı ise 0.183 (CC) ve 0.413 (GG) aralığında değişmiştir. CG genotip frekansı 0.404 olarak hesaplanmıştır. HSP90AA1 bölgesindeki varyasyonların korunması gelecekte ısı stresine karşı yapılacak olan seleksiyon programlarının planlanması için gerekli olacaktır.Sonuç: HSP90AA1 ve benzeri genler yakın gelecekte iklim değişikliğinden kaynaklana ısı stresinin azaltılması için yeni fırsatlar sunabilmektedir. Ayrıca, Pırlak koyunlarında koruma çalışmalarının şekillendirilmesi için çevresel stresörlerle ilişkili diğer genomik bölgeler temelinde daha fazla çalışmanın yapılması gerekmektedir.

Pırlak sheep AS-PCR characterization heat stress heat tolerance HSP90AA1 polymorphism

Pırlak koyunu AS-PZR karakterizasyon ısı stresi ısı toleransı HSP90AA1 polimorfizm

Scientific and Technological Research Council of Turkey

1919B012101337

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