Objective: This study aimed to determine the effect of clove oil on in vitro dry matter digestibility (DMD) and metabolizable energy (ME) values of wheat straw. ,
Material and Methods: In the research, clove oil (66.6% eugenol) was treated with wheat straw at different doses (0, 50, 100, 200 ppm) and hours (1, 3, 5, 7, 9, 12). Firstly, in vitro cellulase method was applied in the study. In the application of the method, pepsin (2000 FIP-U/g) and also the cellulase enzyme obtained from Trichoderma viride (Onozuka R-10, 1 U/mg activity) were used. Then from obtained values, the DMD value of the feed and the amount of organic matter soluble in the enzyme (ELOS) were found and the in vitro ME values were calculated.
Results: Clove etheric oil depending on the increasing dose was increased DMD, ELOS and ME values of wheat straw (P<0.01). Accordingly, clove oil treatment, increased the DMD, ELOS and ME values of the straw from 37.17% to 53.92%, from 22.31% to 39.45% and from 7.53 MJ/kg to 8.13 MJ/kg, respectively (P<0.01). The highest values for these parameters were obtained at 200 ppm dose and 5 hours (P<0.01, P<0.05).
Conclusion: As a result, clove oil treatment in wheat straw has an important potential effect in increasing the in vitro digestion and energy value of feed. However, the results of the study should be supported by more detailed studies, especially in vivo studies.
Amaç:
Bu çalışmada, karanfil eterik yağının buğday samanının in vitro KMS ve ME değerlerine etkisinin belirlenmesi amaçlanmıştır.
Materyal
ve Metot: Araştırmada, karanfil yağı (%66.6 eugenol), farklı
doz (0, 50, 100, 200 ppm) ve saatlerde (1,3,5,7,9,12) buğday samanı ile muamele
edilmiştir. Çalışmada, öncelikle in vitro Selülaz yöntemi uygulanmıştır. Yöntemin uygulanmasında Trichoderma
viride’ den (Onozuka R-10, 1 U/mg aktivite) elde edilen Selülaz enzimi ile
Pepsin (2000 FIP-U/g) kullanılmıştır. Daha sonra elde edilen değerlerden, yemin
KMS değeri ile enzimde çözünebilen organik madde (ELOS) miktarı bulunmuş ve in
vitro ME değerleri hesaplanmıştır.
Bulgular:
Karanfil
eterik yağı artan doza bağlı olarak, buğday samanının KMS, ELOS ve ME değerlerini
artırmıştır (P<0.05). Buna göre, karanfil yağı muamelesi, samanın KMS, ELOS
ve ME değerlerini sırasıyla %37.17 den %53.92 ye, %22.31 den %39.45 e ve 7.53
MJ/kg dan 8.13 MJ/kg a yükseltmiştir (P<0.05). Bu parametrelere ait en
yüksek değerler, 200 ppm doz ve 5. saatte elde edilmiştir.
Primary Language | Turkish |
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Journal Section | Research Articles |
Authors | |
Publication Date | June 28, 2019 |
Submission Date | November 19, 2018 |
Published in Issue | Year 2019 Volume: 60 Issue: 1 |
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