Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study

Year 2021, , 71 - 75, 14.10.2021

Necip Altundaş Erdal Karagülle , Emre Karakaya

https://doi.org/10.52827/hititmedj.967106

Abstract

Objective: Disruption of the intestinal epithelial barrier has been shown to occur following burn injury. This process can lead to translocation of pathogens from the gut lumen to the systemic circulation and distant organs thereby increasing the risk for sepsis. The aim of this study was to examine the effect of amifostine (WR-2721) on bacterial translocation in a rat burn injury model.

Material and Method: A total of 27 male Wistar albino rats were divided into three groups of nine. Group I was a control group. Group II and Group III was subjected to third-degree burns over 30% of the total body surface area, and group III was administered amifostine 200 ml/kg intraperitoneally, followed by a 10 ml/kg/day maintenance dose after undergoing third-degree burns. After 48 hours, tissue and blood samples were obtained and cultured from the liver, spleen, mesenteric lymph nodes, and cecum.

Results: Blood cultures were negative in all groups. In the control group, colonization appeared only in the cecum, but in groups II and III, colonization was found in the liver, spleen, mesenteric lymph nodes, and cecum. While bacterial colonization was most frequently found in the cecum and mesenteric lymph nodes, bacterial counts did not significantly differ in the cecum (P = 0.298) and mesenteric lymph nodes (P = 0.418) between groups II and III.

Conclusion: Amifostine alone is not effective in controlling bacterial translocation associated with burn injuries. These results should be interpreted with caution as there are a number of factors that affect bacterial translocation.

Keywords

Amifostine, Bacterial translocation, Burns

Yanık Sonrası Gelişen Bakteriyel Translokasyon Üzerine Amifostin’in (WR-2721) Etkisi

Abstract

Amaç: Bağırsak epitel bariyerinin bozulmasının yanık yaralanmasını takiben meydana geldiği gösterilmiştir. Bu süreç, patojenlerin bağırsak lümeninden sistemik dolaşıma ve uzak organlara yer değiştirmesine yol açarak sepsis riskini artırır. Bu çalışmanın amacı, sıçan yanığı yaralanma modelinde amifostin'in (WR-2721) bakteriyel translokasyon üzerindeki etkisini incelemektir.

Gereç ve Yöntemler: Toplam 27 erkek Wistar albino sıçanı dokuzlu üç gruba ayrıldı. Grup I bir kontrol grubuydu. Grup II ve grup III, toplam vücut yüzey alanının %30'u üzerinde üçüncü derece yanıklara maruz bırakıldı ve grup III'e intraperitoneal olarak 200 ml/kg amifostin uygulandı, ardından üçüncü derece yanıklardan sonra 10 ml/kg/gün idame dozu uygulandı. 48 saat sonra karaciğer, dalak, mezenterik lenf düğümleri ve çekumdan doku ve kan örnekleri alındı ve kültür ekimi yapıldı.

Bulgular: Kan kültürleri tüm gruplarda negatifti. Kontrol grubunda kolonizasyon sadece çekumda görülürken, grup II ve III'te karaciğer, dalak, mezenterik lenf nodları ve çekumda kolonizasyon tespit edildi. Bakteriyel kolonizasyon en sık çekum ve mezenterik lenf düğümlerinde bulunurken, grup II ve III arasında çekum (P = 0.298) ve mezenterik lenf düğümlerinde (P = 0.418) bakteri sayıları önemli ölçüde farklılık göstermedi.

Sonuç: Amifostin tek başına yanık yaralanmaları ile ilişkili bakteriyel translokasyonu kontrol etmede etkili değildir. Bakteriyel translokasyonu etkileyen bir dizi faktör olduğu için bu sonuçlar dikkatle yorumlanmalıdır.

Keywords

yanıklar, Amifostin, Bakteriyel Translokasyon, Yanıklar

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