Kolon Adenokarsinomlarinda Kras Mutasyonlarinin Sikliği Ve Lenf Nodu Metastazi İle İlişkisi

Year 2020, Volume: 2 Issue: 2, 36 - 41, 23.06.2020

Gökhan Varlı , İbrahim Hanifi Özercan , Ebru Önalan

Abstract

Amaç: Gastrointestinal sistemin en
yaygın malignitesi olan kolon kanserleri mortalite ve morbiditenin ana
nedenlerinden biridir. Kolorektal kanserlerin %30-40’ında KRAS gen mutasyonu
izlendiğinden tedaviye başlamadan önce mutasyon taraması önemlidir. Bu
çalışmadaki amacımız orta derece diferansiye kolon adenokarsinomlarında KRAS
kodon 12, 13, 61 mutasyon durumunu belirlemek ve bu mutasyonların lenf nodu
metastazı ile ilişkisini araştırmaktır.

Gereç ve yöntem: Fırat Üniversitesi Tıp Fakültesi
Tıbbi Patoloji Anabilim Dalı arşivinden seçilen 60 adet lenf nodu metastazlı ve
60 adet lenf nodu metastazı olmayan toplam 120 adet orta derecede diferansiye
kolon adenokarsinomu örneğinden uygun primer çiftleri PZR ile çoğaltılıp sekans
analizi ile KRAS mutasyon durumu belirlenmiştir.

Bulgular: Çalışmamıza alınan 120 olguda 40
tanesi kodon 12 de ve 5 tanesi kodon 13 de olmak üzere %37.5 (45/120) oranında
KRAS mutasyonu izlendi. KRAS kodon 61 de mutasyona rastlanmadı.

Sonuç: Çalışmamızda KRAS mutasyon durumu
ile lenf nodu metastazı arasında anlamlı bir ilişki bulunmadı. Bu çalışma küçük
bir alanda öncesinde tanı almış sınırlı sayıda olguda yapıldı; ancak çok
merkezli ve daha geniş çalışmalar için bir ön izlenim taşıma özelliğindedir.

Keywords

Kolon adenokarsinomu, kras, lenf nodu metastazı

Supporting Institution

Fırat Üniversitesi Girişimsel Olmayan Etik Kurul Başkanlığı

Project Number

TF.14.08

Incidence Of Kras Mutations In Colon Adenocarcinomas And Its Association With Lymph Node Metastases

Abstract

Objective: Colon adenocarcinoma, one of the
most frequent malignant tumors of the gastrointestinal system, is a major cause
of morbidity and mortality. As KRAS mutations are encountered in 30-40% of
colorectal carcinomas, a mutation screening is required before therapy
commences. In this study, we aim to determine KRAS codon 12, 13 and 61
mutations in moderately differentiated colon adenocarcinomas and assess whether
these mutations are associated with lymph node metastases.

Materials and methods: A total of 120 moderately differentiated colorectal carcinomas, 60 with
lymph node metastases and 60 without, were included. Samples underwent PCR with
appropriate primers and Sanger sequencing was carried out to determine their
KRAS mutation status.

Results: Out of the 120 cases included in our study,
40 carried codon 12, 5 carried codon 13 mutations. In total, 37.5% of cases had
a KRAS mutation (45/120). No mutation was detected in codon 61.

Conclusion: Our
study has not shown a significant association between the presence of KRAS
mutations and lymph node metastasis. This study was conducted in a limited
number of patients and in a pre-defined, small area; but can be used as a
preliminary step for multicenter and larger studies.

Keywords

colon adenocarcinoma, kras, lymph node metastases

Project Number

TF.14.08

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