Assessment of the Cytotoxicity of Melia azedarach L. Extracts on Human Adipose-derived Mesenchymal Stem Cells

Year 2018, Volume: 46 Issue: 1, 121 - 128, 01.03.2018

Burcu Efe Yusuf Furkan Galata Yavuz Emre Aslan

Abstract

I
n this study, aqueous extracts of Melia azedarach L. green fruit and leaves were obtained using two different
extraction methods. The extraction yields of the green fruit and leaves were found as 24.11% and 37.98%
for the infusion method; 17.76% and 27.00% for the rotating method, respectively. The total phenolic content,
related to the infusion method, was ascertained for green fruit extract 173.67±10.84 mg Gallic Acid Equivalent
(GAE)/g dry weight and leaf extract 312.33±9.81 mg GAE/g dry weight. In other respects, antioxidant activity
related to the infusion method was determined for green fruit extract 172.51±13.23 mg Trolox/L and leaf extract
569.16±10.41 mg Trolox/L. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to identify
the chemical composition of the extracts. The cytotoxicity levels of the extracts were assessed on human
adipose-derived mesenchymal stem cells (hAMSCs) using commercially available XTT assay. Consequently, it
has been found that the green fruit extract has more cytotoxic activity than the leaf extract on hAMSCs.

Keywords

Melia azedarach L., toplam fenolik bileşikler, sitotoksisite, insan adipoz kaynaklı mezenkimal kök hücreler

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