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Year 2019, Volume: 47 Issue: 2, 185 - 192, 18.09.2019
https://doi.org/10.15671/hjbc.623783

Abstract

References

  • 1. I. Smetanska, Production of secondary metabolites using plant cell cultures, Adv. Biochem. Eng. Biotechnol., 111 (2008) 187-228.
  • 2. M.Singh, B.Roy, V. Tandon, R. Chaturvedi, Extracts of dedifferentiated cultures of Spilanthes acmella Murr. possess antioxidant and anthelmintic properties and hold promise as an alternative source of herbal medicine, Plant Biosyst., 148 (2014) 259-267.
  • 3. M.S. Hussain, S. Fareed, S. Ansari, M.A. Rahman, I.Z. Ahmad, M. Saeed, Current approaches toward production of secondary plant metabolites, J. Pharm. Bioallied. Sci., 4 (2012) 10-20.
  • 4. P. Nartop, A. Gürel, Immobilization of Plant Cells and Its Effects on Secondary Metabolite and Biomass Production, Biotechnology and Genetic Engineering II of Recent Progress in Medicinal Plants Series, Vol.39:1-933699-99-X Series, ISBN:0-9656038-5-7, 2014, Studium Press.
  • 5. P. Nartop, Ş. Akay, A. Gürel, Immobilization of Rubia tinctorum L. suspension cultures and its effects on alizarin and purpurin accumulation and biomass production, Plant Cell Tiss. Org. Cult., 112 (2013) 123-128.
  • 6. P.H. Davis, Flora of Turkey and East Aegean Islands, University Press, Edinburgh, 1970, 3, 164.
  • 7. E. Bedir, I. Çalış, S. Piacente, C. Pizza, IA. Khan, New flavonol glycoside from the aerial parts of Astragalus vulneraria, Chem. Pharm. Bull., 48(12) (2000a) 1994-1995.
  • 8. E. Bedir, I. Çalış, R. Aquino, S. Piacente, C. Pizza, Secondary metabolites from the roots of Astragalus trojanus. J. Nat. Prod., 62 (1999) 563-568.
  • 9. E. Bedir, I. Çalış, R. Aquino, S. Piacente, C. Pizza, Trojanoside H: A cycloartane-type glycoside from the aerial parts of Astragalus trojanus, Phytochemistry, 51 (1999) 1017-1020.
  • 10. E. Bedir, I. Tatlı, I. Çalış, I.A. Khan, Trojanosides I-K: New cycloartane-type glycosides from the aerial parts of Astragalus trojanus, Chem. Pharm. Bull., 49 (2001) 1482- 1486.
  • 11. T. Savran, D. Gülcemal, M. Masullo, T. Karayıldırım, E. Polat, S. Piacente, Ö. Alankuş-Çalışkan, Cycloartane Glycosides from Astragalus erinaceus, Rec. Nat. Prod., 6 (2012) 230-236.
  • 12. Y. Cai, Q. Luo, M. Sun, H. Corke, Antioxidant activity and phenolic compounds of 112, traditional chinese medicinal plants associated with anticancer, Life Sci., 74 (2004) 2157- 2184.
  • 13. E. Bedir, N. Pugh, I. Çalış, D.S. Pasco, I.A. Khan, Immunostimulatory effects of cycloartane-type triterpene glycosides from Astragalus species, Biol. Pharm. Bull., 23 (2000b) 834-837.
  • 14. S. Hasançebi, N. Turgut-Kara, Ö. Çakır, Ş. Arı, Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae), Turk. J. Bot., 35 (2011) 203–210.
  • 15. A. Nalbantsoy, T. Nesil, Ö. Yılmaz-Dilsiz, G. Aksu, S. Khan, E. Bedir, Evaluation of the immunomodulatory properties in mice And ın vitro anti-Inflammatory activity of cycloartanetype saponins from Astragalus species, J. Ethnopharmacol., 139 (2012) 574–581.
  • 16. H.F. Valenzuela, T. Fuller, J. Edwards, D. Finger, B. Molgora, Cycloastragenol extends T-cell proliferation by increasing telomerase activity, J. Immunol., 182 (2009) 90–30.
  • 17. S. Wang, H. Huang, W. Gao, C. Zhuang, B. Li, P. Zhou, D. Kong, Anti-hepatitis B virus activities of astragaloside IV isolated from Radix Astragali, Biol. Pharm. Bull., 32 (2009) 132–135.
  • 18. P. Nartop, A. Gürel, I.H.Akgün, E. Bedir, Astragaloside IV and cycloastragenol production capacity of Astragalus trojanus calli, Rec. Nat. Prod., 9 (2015) 49-61.
  • 19. A.M.A. Abd El-Mavla, Production of cycloartane triterpene glycosides in shoot cultures of Astragalus sieberi DC, Nat. Prod. Res., 24 (2010) 416-422.
  • 20. S. Erişen, E. Atalay, M. Yorgancılar, The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey, Turk. J. Bot., 35 (2011) 521-526.
  • 21. I. Ionkova, T. Kartnig, W. Alfermann, Cycloartane saponin production in hairy root cultures of Astragalus mongholicus, Phytochemistry, 45 (1997) 1597-1600.
  • 22. M. Du, X.J. Wu, J. Ding, Z.B. Hu, K.N. White, C.J. BranfordWhite, Astragaloside IV and polysaccharide production by hairy roots of Astragalus membranaceus in bioreactors, Biotechnol. Lett., 25 (2003) 1853-1856.
  • 23. G.B. Lloyd, B.H. McCown, Commercial-feasible micropropagation of mountain laurel- kalmia latifolia by use of shoot- tip culture. Proc. Int. Plant Prop. Soc., 30 (1980) 421-427.
  • 24. N.K. Ziebur, A. Shrift, Response to selenium by callus cultures derived from Astragalus species. Plant Physiol., 47(4) (1971) 545–550.
  • 25. D.D.Y. Ryu, S.O. Lee, R.J. Romani, Determination of growth rate for the plant cell cultures: Comparative studies, Biotechnol. Bioengin., 35 (1990) 305-311.
  • 26. R. Krishnan, V.S.A. Kumar, K. Murugan, Establishment of cell suspension culture in Marchantia linearis Lehm&Lindenb. for the optimum production of flavonoids, Biotech. (2013) DOI 10.1007/s13205-013-0123-7.
  • 27. N.C. Vora, Y.T. Jasrai, Natural and low-cost substitutes of synthetic PGR for micropropagation of banana. CIBTech. J. Biotechn., 2 (2012) 9-13.
  • 28. Q. Liu, L. Cui, Y. Guo, X. Ni, Y, Zhang, G. Kai, Optimization of nutritive factors in culture media for growth and tropane alkaloid production from Anisodus acutangulus hairy roots, J. Appl. Pharm. Sci., 3 (2013) 1-4.
  • 29. I-S. Park, D-I. Kim, Significance of fresh weight to dry cell weight ratio in plant cell suspension cultures. Biotechnol. Tech., 7 (1993) 627-630.
  • 30. L.A. Caro, P.A. Polci, L.I. Lindstrom, C.V. Echenique, L.F. Hernandez, Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants, Biocell, 26 (2002) 25- 33.
  • 31. S. Pola, S.N. Mani, T. Ramana, Long-term maintanence of callus cultures form ımmature embryo of Sorghum bicolor, World J. Agric. Sci., 5 (2009) 415-421.
  • 32. A.T. Roy, A. Koutoulis, D.N. De, Cell Suspension Culture and Plant Regeneration in The Latex-Producing Plant, Calotropis gigantea (Linn.) R. Br. Plant Cell Tiss. Org. Cult., 63 (2000) 15-22.
  • 33. E.F. George, G-J. de Klerk, The Components of Plant Tissue Culture Media I: Macro- and Micro-Nutrients. In George EF, Hall MA, de Klerk G-J, eds., Plant Propagation by Tissue Culture, 3rd Edition, Vol. 1. The Background. Springer-Verlag, Dordrecht (2008) pp.65-113.
  • 34. Z. Molnar, E. Virag, V. Ördög, Natural substances in tissue culture media of higher plants. Acta Biologica Szegediensis, 55 (2011) 123-127.
  • 35. W. Li, J.F. Fitzloff, Determination of astragaloside IV in Radix Astragali (Astragalus membranaceus var. monghulicus) using high-performance liquid chromatography with evaporative light-scattering detection, J. Chromatogr. Sci., 39 (2001) 459-462.

Astragalus Trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate

Year 2019, Volume: 47 Issue: 2, 185 - 192, 18.09.2019
https://doi.org/10.15671/hjbc.623783

Abstract

I
n this study, two grams of callus regenerated from stem and leaf explants of Astragalus trojanus Stev. were cultured in Woody Plant Medium (WPM) supplemented with 1 mg/L 2,4-D for four weeks and used as inoculum in order to investigate the
effects of working volume and media composition. The highest biomass was obtained in 250 mL flask with astragaloside IV
(1.66 µg/mg) and cycloastragenol (0.19 µg/mg) accumulation. Different concentrations of sucrose and casein hydrolysate (1
and 2 g/L) were also tested and the effect of pH was also investigated. Biomass accumulation cannot be enhanced, however,
astragaloside IV and cycloastragenol content was ascended. The highest astragaloside IV (95.23 µg/mg) and cycloastragenol
(5.93 mg/mg) accumulations were obtained at pH 6.8 and 2 g/L casein hydrolysate, respectively

References

  • 1. I. Smetanska, Production of secondary metabolites using plant cell cultures, Adv. Biochem. Eng. Biotechnol., 111 (2008) 187-228.
  • 2. M.Singh, B.Roy, V. Tandon, R. Chaturvedi, Extracts of dedifferentiated cultures of Spilanthes acmella Murr. possess antioxidant and anthelmintic properties and hold promise as an alternative source of herbal medicine, Plant Biosyst., 148 (2014) 259-267.
  • 3. M.S. Hussain, S. Fareed, S. Ansari, M.A. Rahman, I.Z. Ahmad, M. Saeed, Current approaches toward production of secondary plant metabolites, J. Pharm. Bioallied. Sci., 4 (2012) 10-20.
  • 4. P. Nartop, A. Gürel, Immobilization of Plant Cells and Its Effects on Secondary Metabolite and Biomass Production, Biotechnology and Genetic Engineering II of Recent Progress in Medicinal Plants Series, Vol.39:1-933699-99-X Series, ISBN:0-9656038-5-7, 2014, Studium Press.
  • 5. P. Nartop, Ş. Akay, A. Gürel, Immobilization of Rubia tinctorum L. suspension cultures and its effects on alizarin and purpurin accumulation and biomass production, Plant Cell Tiss. Org. Cult., 112 (2013) 123-128.
  • 6. P.H. Davis, Flora of Turkey and East Aegean Islands, University Press, Edinburgh, 1970, 3, 164.
  • 7. E. Bedir, I. Çalış, S. Piacente, C. Pizza, IA. Khan, New flavonol glycoside from the aerial parts of Astragalus vulneraria, Chem. Pharm. Bull., 48(12) (2000a) 1994-1995.
  • 8. E. Bedir, I. Çalış, R. Aquino, S. Piacente, C. Pizza, Secondary metabolites from the roots of Astragalus trojanus. J. Nat. Prod., 62 (1999) 563-568.
  • 9. E. Bedir, I. Çalış, R. Aquino, S. Piacente, C. Pizza, Trojanoside H: A cycloartane-type glycoside from the aerial parts of Astragalus trojanus, Phytochemistry, 51 (1999) 1017-1020.
  • 10. E. Bedir, I. Tatlı, I. Çalış, I.A. Khan, Trojanosides I-K: New cycloartane-type glycosides from the aerial parts of Astragalus trojanus, Chem. Pharm. Bull., 49 (2001) 1482- 1486.
  • 11. T. Savran, D. Gülcemal, M. Masullo, T. Karayıldırım, E. Polat, S. Piacente, Ö. Alankuş-Çalışkan, Cycloartane Glycosides from Astragalus erinaceus, Rec. Nat. Prod., 6 (2012) 230-236.
  • 12. Y. Cai, Q. Luo, M. Sun, H. Corke, Antioxidant activity and phenolic compounds of 112, traditional chinese medicinal plants associated with anticancer, Life Sci., 74 (2004) 2157- 2184.
  • 13. E. Bedir, N. Pugh, I. Çalış, D.S. Pasco, I.A. Khan, Immunostimulatory effects of cycloartane-type triterpene glycosides from Astragalus species, Biol. Pharm. Bull., 23 (2000b) 834-837.
  • 14. S. Hasançebi, N. Turgut-Kara, Ö. Çakır, Ş. Arı, Micropropagation and root culture of Turkish endemic Astragalus chrysochlorus (Leguminosae), Turk. J. Bot., 35 (2011) 203–210.
  • 15. A. Nalbantsoy, T. Nesil, Ö. Yılmaz-Dilsiz, G. Aksu, S. Khan, E. Bedir, Evaluation of the immunomodulatory properties in mice And ın vitro anti-Inflammatory activity of cycloartanetype saponins from Astragalus species, J. Ethnopharmacol., 139 (2012) 574–581.
  • 16. H.F. Valenzuela, T. Fuller, J. Edwards, D. Finger, B. Molgora, Cycloastragenol extends T-cell proliferation by increasing telomerase activity, J. Immunol., 182 (2009) 90–30.
  • 17. S. Wang, H. Huang, W. Gao, C. Zhuang, B. Li, P. Zhou, D. Kong, Anti-hepatitis B virus activities of astragaloside IV isolated from Radix Astragali, Biol. Pharm. Bull., 32 (2009) 132–135.
  • 18. P. Nartop, A. Gürel, I.H.Akgün, E. Bedir, Astragaloside IV and cycloastragenol production capacity of Astragalus trojanus calli, Rec. Nat. Prod., 9 (2015) 49-61.
  • 19. A.M.A. Abd El-Mavla, Production of cycloartane triterpene glycosides in shoot cultures of Astragalus sieberi DC, Nat. Prod. Res., 24 (2010) 416-422.
  • 20. S. Erişen, E. Atalay, M. Yorgancılar, The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey, Turk. J. Bot., 35 (2011) 521-526.
  • 21. I. Ionkova, T. Kartnig, W. Alfermann, Cycloartane saponin production in hairy root cultures of Astragalus mongholicus, Phytochemistry, 45 (1997) 1597-1600.
  • 22. M. Du, X.J. Wu, J. Ding, Z.B. Hu, K.N. White, C.J. BranfordWhite, Astragaloside IV and polysaccharide production by hairy roots of Astragalus membranaceus in bioreactors, Biotechnol. Lett., 25 (2003) 1853-1856.
  • 23. G.B. Lloyd, B.H. McCown, Commercial-feasible micropropagation of mountain laurel- kalmia latifolia by use of shoot- tip culture. Proc. Int. Plant Prop. Soc., 30 (1980) 421-427.
  • 24. N.K. Ziebur, A. Shrift, Response to selenium by callus cultures derived from Astragalus species. Plant Physiol., 47(4) (1971) 545–550.
  • 25. D.D.Y. Ryu, S.O. Lee, R.J. Romani, Determination of growth rate for the plant cell cultures: Comparative studies, Biotechnol. Bioengin., 35 (1990) 305-311.
  • 26. R. Krishnan, V.S.A. Kumar, K. Murugan, Establishment of cell suspension culture in Marchantia linearis Lehm&Lindenb. for the optimum production of flavonoids, Biotech. (2013) DOI 10.1007/s13205-013-0123-7.
  • 27. N.C. Vora, Y.T. Jasrai, Natural and low-cost substitutes of synthetic PGR for micropropagation of banana. CIBTech. J. Biotechn., 2 (2012) 9-13.
  • 28. Q. Liu, L. Cui, Y. Guo, X. Ni, Y, Zhang, G. Kai, Optimization of nutritive factors in culture media for growth and tropane alkaloid production from Anisodus acutangulus hairy roots, J. Appl. Pharm. Sci., 3 (2013) 1-4.
  • 29. I-S. Park, D-I. Kim, Significance of fresh weight to dry cell weight ratio in plant cell suspension cultures. Biotechnol. Tech., 7 (1993) 627-630.
  • 30. L.A. Caro, P.A. Polci, L.I. Lindstrom, C.V. Echenique, L.F. Hernandez, Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants, Biocell, 26 (2002) 25- 33.
  • 31. S. Pola, S.N. Mani, T. Ramana, Long-term maintanence of callus cultures form ımmature embryo of Sorghum bicolor, World J. Agric. Sci., 5 (2009) 415-421.
  • 32. A.T. Roy, A. Koutoulis, D.N. De, Cell Suspension Culture and Plant Regeneration in The Latex-Producing Plant, Calotropis gigantea (Linn.) R. Br. Plant Cell Tiss. Org. Cult., 63 (2000) 15-22.
  • 33. E.F. George, G-J. de Klerk, The Components of Plant Tissue Culture Media I: Macro- and Micro-Nutrients. In George EF, Hall MA, de Klerk G-J, eds., Plant Propagation by Tissue Culture, 3rd Edition, Vol. 1. The Background. Springer-Verlag, Dordrecht (2008) pp.65-113.
  • 34. Z. Molnar, E. Virag, V. Ördög, Natural substances in tissue culture media of higher plants. Acta Biologica Szegediensis, 55 (2011) 123-127.
  • 35. W. Li, J.F. Fitzloff, Determination of astragaloside IV in Radix Astragali (Astragalus membranaceus var. monghulicus) using high-performance liquid chromatography with evaporative light-scattering detection, J. Chromatogr. Sci., 39 (2001) 459-462.
There are 35 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Pınar Nartop

Aynur Gürel This is me

İsmail Hakkı Akgün This is me

Erdal Bedir This is me

Publication Date September 18, 2019
Acceptance Date February 12, 2019
Published in Issue Year 2019 Volume: 47 Issue: 2

Cite

APA Nartop, P., Gürel, A., Akgün, İ. H., Bedir, E. (2019). Astragalus Trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. Hacettepe Journal of Biology and Chemistry, 47(2), 185-192. https://doi.org/10.15671/hjbc.623783
AMA Nartop P, Gürel A, Akgün İH, Bedir E. Astragalus Trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. HJBC. September 2019;47(2):185-192. doi:10.15671/hjbc.623783
Chicago Nartop, Pınar, Aynur Gürel, İsmail Hakkı Akgün, and Erdal Bedir. “Astragalus Trojanus Stev. Batch Cultures: Cycloartane-Type Metabolite Accumulation in Response to PH, Sucrose and Casein Hydrolysate”. Hacettepe Journal of Biology and Chemistry 47, no. 2 (September 2019): 185-92. https://doi.org/10.15671/hjbc.623783.
EndNote Nartop P, Gürel A, Akgün İH, Bedir E (September 1, 2019) Astragalus Trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. Hacettepe Journal of Biology and Chemistry 47 2 185–192.
IEEE P. Nartop, A. Gürel, İ. H. Akgün, and E. Bedir, “Astragalus Trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate”, HJBC, vol. 47, no. 2, pp. 185–192, 2019, doi: 10.15671/hjbc.623783.
ISNAD Nartop, Pınar et al. “Astragalus Trojanus Stev. Batch Cultures: Cycloartane-Type Metabolite Accumulation in Response to PH, Sucrose and Casein Hydrolysate”. Hacettepe Journal of Biology and Chemistry 47/2 (September 2019), 185-192. https://doi.org/10.15671/hjbc.623783.
JAMA Nartop P, Gürel A, Akgün İH, Bedir E. Astragalus Trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. HJBC. 2019;47:185–192.
MLA Nartop, Pınar et al. “Astragalus Trojanus Stev. Batch Cultures: Cycloartane-Type Metabolite Accumulation in Response to PH, Sucrose and Casein Hydrolysate”. Hacettepe Journal of Biology and Chemistry, vol. 47, no. 2, 2019, pp. 185-92, doi:10.15671/hjbc.623783.
Vancouver Nartop P, Gürel A, Akgün İH, Bedir E. Astragalus Trojanus Stev. Batch Cultures: Cycloartane-type Metabolite Accumulation in Response to pH, Sucrose and Casein Hydrolysate. HJBC. 2019;47(2):185-92.

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