Modified Graphite Surfaces Prepared for Electrochemical Biomolecular Interaction Detection Studies

Year 2019, Volume: 47 Issue: 4, 445 - 454, 01.12.2019

Doç. Dr. Filiz Kuralay

https://doi.org/10.15671/hjbc.676957

Abstract

Bu çalışma, çift sarmal DNA (dsDNA) ile önemli ve sık kullanılan bir antikanser ilacı olan Mitomisin C (MMC) arasındaki biyomoleküler etkileşimin görüntülenmesi için elektroaktif polimer modifiye elektrot malzemelerinin hazırlanmasını göstermektedir. Modifiye elektrot malzemeleri, o-fenilendiamin (oPD) monomerinin nanomalzeme içeren bir çözeltide elektropolimerizasyonu ile oluşturulmuştur. Katkı maddesi (dopant) molekül olarak kullanılan nanomalzeme grafen (GN)’dir ve elektropolimerizasyon tekniği dönüşümlü voltametri (CV)’dir. Sonrasında poli(o-fenilendiamin) polimer modifiye yüzeylere dsDNA immobilizasyonu gerçekleştirilmiştir. Oluşturulan nanomalzeme katılmış bu polimer modifiye elektrotlar, dsDNA-MMC etkileşiminin tespitinde biyotayin platformları olarak kullanılmışlardır. Biyomoleküler etkileşimlerini aydınlatmak için farklı MMC etkileşim süreleri çalışılmıştır.

Keywords

Modified electrodes, Electropolymerization, dsDNA, Mitomycin C

Supporting Institution

Türkiye Bilimler Akademisi

Thanks

F. Kuralay acknowledges Turkish Academy of Sciences (TÜBA) as an associate member and TÜBA-GEBİP program for financial support. F. Kuralay also acknowledges Yaşar Bayramlı for his technical support.

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