Year 2020, Volume 48 , Issue 2, Pages 119 - 123 2020-04-19

Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer

Meltem DEMİREL KARS [1]


Meme kanseri kök hücrelerinin kendi kendini yenilemesi, kendiliğinden olan ve sonradan kazanılan ilaç direnci, meme kanserinin tedavisi stratejilerini önleyen ana faktörlerdir. Yeni terapötik ajanlar geliştirmek için yapılan çalışmalarda hedeflenecek olan biyobelirteç molekülleri araştırılmaktadır. Bu çalışmada, meme kanseri kök hücrelerinin (MKKH) özelliklerini taşıyan, antikanser ilaç-paklitaksele dirençli meme kanseri hücre hattında tüm genom cDNA mikrodizin analizi yapılmıştır. İlaca duyarlı parental MCF-7 hücre hattı kontrol grubu olarak kullanılmıştır. MKKH benzeri hücrelerde SLC taşıyıcı proteinleri kodlayan ve aşırı ifade edilen genler analiz edilmiştir. Elde edilen sonuçlara göre, 21 adet SLC proteini kodlayan genlerin ifade düzeyleri 2.0 ve 35 kat arasında artmıştır.  SLC38A5, SLC43A3, SLC6A15, SLC1A1, SLC2A3, SLC26A2, SLC22A15, SLC16A3 genlerinin ifade düzeyleri 9 ile 35 kat arasında artmıştır. Dolayısıyla, ilgili proteinler potansiyel hedefler olarak kabul edilebilir ve bu bilgiler, meme kanseri kök hücrelerini hedef alan biyobelirteçlerin keşfedilmesi için yeni bir araştırma alanı açacaktır.

 

Solute carrier proteins, microarray, breast cancer stem cells, drug resistance
  • [1] E. Perland, R. Fredriksson, Classification Systems of Secondary Active Transporters, Trends Pharmacol. Sci., 38 (2017) 305–315.
  • [2] M. Rask-Andersen, S. Masuram, R. Fredriksson, H.B. Schiöth, Solute carriers as drug targets: current use, clinical trials and prospective, Mol. Aspects Med., 34 (2013) 702-710.
  • [3] F.S. Liu, Mechanisms of chemotherapeutic drug resistance in cancer therapy--a quick review, Taiwan J. Obstet. Gynecol., 48 (2009) 239-244.
  • [4] M.D. Kars, Ö.D. İşeri, U. Gunduz, A.U. Ural, F. Arpacı, J. Molnar, Development of rational in vitro models for drug resistance in breast cancer and modulation of MDR by selected compounds, Anticancer Res., 26 (2006) 4559-4568.
  • [5] M.D. Kars, Ö.D. İşeri, U. Gündüz, Drug resistant breast cancer cells overexpress ETS1 gene, Biomed. Pharmacother., 64 (2010) 458-462.
  • [6] M.D. Kars, Ö.D. İşeri, U. Gündüz, A microarray based expression profiling of paclitaxel and vincristine resistant MCF-7 cells, Eur. J. Pharmacol., 657 (2011) 4-9.
  • [7] M.D. Kars, G. Yıldırım, Determination of the Target Proteins in Chemotherapy Resistant Breast Cancer Stem Cell-Like Cells by Protein Array, Eur. J. Pharmacology, 848 (2019) 23-29.
  • [8] M.D. Kars Ö.D. İşeri, U. Gündüz, J. Molnar, Reversal of MDR by Synthetic and Natural Compounds in Drug Resistant MCF-7 Cell Lines, Chemotherapy, 54 (2008) 194-200.
  • [9] Ö.D., İşeri M.D Kars., F. Arpacı, U. Gündüz , Gene Expression Analysis of Drug Resistant MCF-7 Cells: Implications for Relation to Extracellular Matrix Proteins, Cancer Chemoth. Pharm., 65 (2010) 447-455.
  • [10] V . Tirino., V. Desiderio, F. Paino,G. Papaccio, M. De Rosa, Methods for Cancer Stem Cell Detection and Isolation, Somatic Stem Cells: Methods and Protocols, Methods Mol. Biol., 879 (2012) 32.
  • [11] P. Valent, D. Bonnet, R. De Maria, T. Lapidot, et al., Cancer stem cell definitions and terminology the devil is in the details, Nat. Rev. Cancer., 12 (2012) 767-775.
  • [12] Y.D. Bhutia, V. Ganapathy, Glutamine transporters in mammalian cells and their functions in physiology and cancer, Biochim. Biophys. Acta., 1863 (2016) 2531-2539.
  • [13] N. Shimozono, M. Jinnin, M. Masuzawa, M. Masuzawa, et al., NUP160 SLC43A3 is a novel recurrent fusion oncogene in angiosarcoma, Cancer Res., 75 (2015) 4458-4465.
  • [14] E. Pedraz-Cuesta, S. Christensen, A.A. Jensen, N.F. Jensen, et al., The glutamate transport inhibitor DL-Threo-β-Benzyloxyaspartic acid (DL-TBOA) differentially affects SN38- and oxaliplatin-induced death of drug-resistant colorectal cancer cells, BMC Cancer., 15 (2015) 411.
  • [15] M. Masin, J. Vazquez, S. Rossi, S. Groeneveld, et al., GLUT3 is induced during epithelial-mesenchymal transition and promotes tumor cell proliferation in non-small cell lung cancer, Cancer Metab., 29 (2014) 11.
  • [16] Ö.D. İşeri, M.D. Kars, F. Arpacı, C. Atalay, I. Pak, U. Gündüz, Drug Resistant MCF-7 Cells Exhibit Epithelial-Mesenchymal Transition Gene Expression Pattern, Biomed. Pharmacother., 65 (2011) 40-45.
  • [17] L.Y. Dimberg, C.G. Towers, K. Behbakht, T.J. Hotz, et al., A Genome-Wide Loss-of-Function Screen Identifies SLC26A2 as a Novel Mediator of TRAIL Resistance, Mol Cancer Res., 15 (2017) 382-394.
  • [18] A.K. Witkiewicz, D. Whitaker-Menezes, A. Dasgupta, N.J. Philp, Using the “reverse Warburg effect” to identify high-risk breast cancer patients: stromal MCT4 predicts poor clinical outcome in triple-negative breast cancers, Cell Cycle, 11 (2012) 1108–1117.
Primary Language en
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0002-7300-4075
Author: Meltem DEMİREL KARS (Primary Author)
Institution: NECMETTİN ERBAKAN ÜNİVERSİTESİ
Country: Turkey


Dates

Publication Date : April 19, 2020

Bibtex @research article { hjbc526094, journal = {Hacettepe Journal of Biology and Chemistry}, issn = {2687-475X}, eissn = {2687-475X}, address = {Hacettepe Üniversitesi Fen Fakültesi, 06532, Beytepe/ ANKARA/ TÜRKİYE}, publisher = {Hacettepe University}, year = {2020}, volume = {48}, pages = {119 - 123}, doi = {10.15671/hjbc.526094}, title = {Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer}, key = {cite}, author = {Demirel Kars, Meltem} }
APA Demirel Kars, M . (2020). Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer . Hacettepe Journal of Biology and Chemistry , 48 (2) , 119-123 . DOI: 10.15671/hjbc.526094
MLA Demirel Kars, M . "Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer" . Hacettepe Journal of Biology and Chemistry 48 (2020 ): 119-123 <https://dergipark.org.tr/en/pub/hjbc/issue/53810/526094>
Chicago Demirel Kars, M . "Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer". Hacettepe Journal of Biology and Chemistry 48 (2020 ): 119-123
RIS TY - JOUR T1 - Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer AU - Meltem Demirel Kars Y1 - 2020 PY - 2020 N1 - doi: 10.15671/hjbc.526094 DO - 10.15671/hjbc.526094 T2 - Hacettepe Journal of Biology and Chemistry JF - Journal JO - JOR SP - 119 EP - 123 VL - 48 IS - 2 SN - 2687-475X-2687-475X M3 - doi: 10.15671/hjbc.526094 UR - https://doi.org/10.15671/hjbc.526094 Y2 - 2020 ER -
EndNote %0 Hacettepe Journal of Biology and Chemistry Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer %A Meltem Demirel Kars %T Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer %D 2020 %J Hacettepe Journal of Biology and Chemistry %P 2687-475X-2687-475X %V 48 %N 2 %R doi: 10.15671/hjbc.526094 %U 10.15671/hjbc.526094
ISNAD Demirel Kars, Meltem . "Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer". Hacettepe Journal of Biology and Chemistry 48 / 2 (April 2020): 119-123 . https://doi.org/10.15671/hjbc.526094
AMA Demirel Kars M . Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer. HJBC. 2020; 48(2): 119-123.
Vancouver Demirel Kars M . Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer. Hacettepe Journal of Biology and Chemistry. 2020; 48(2): 119-123.
IEEE M. Demirel Kars , "Implications from a microarray analysis: Solute carrier proteins may be potential targets to combat stemness of breast cancer", Hacettepe Journal of Biology and Chemistry, vol. 48, no. 2, pp. 119-123, Apr. 2020, doi:10.15671/hjbc.526094