Bioassays of Entomopathogen Nematode Steinernema feltiae All Type Rhabditida: Steinernematidae and Heterorhabditis bacteriophora Tur- H2 Rhabditida: Heterorhabditidae

Year 2007, Volume: 35 Issue: 1, 39 - 44, 01.01.2007

Işık Oğuzoğlu Nurdan Özer

Abstract

Steinernema feltiae Filipjev 1934 Rhabditida: Steinernematidae which was isolated on the Black Sea coast as the first record of entomopathogenic nematodes, has been cultivated for almost ten years in the Ecological Sciences laboratories in Biology Department of Hacettepe University since its first isolation. In this study, the infection potential of S. feltiae All type isolate has been evaluated in different conditions and compared with Heterorhabditis bacteriophora Poinar, 1970 Rhabditida: Heterorhabditidae , o another entomopathogenic nematode species. The optimum temperature has been shown to be 25 C. A longer contact time resulted in higher infection efficiency. A smaller diameter of the petri-dishes where the host and nematodes were in contact, also increased the efficiency. Among the arthropod hosts, Plodia interpunctella Lepidoptera: Pyralidae showed the highest susceptibility after Galleria mellonella Lepidoptera: Galleridae which is the most appropriate host for entomopathogen nematodes. Musca domestica Diptera: Muscidae and Aedes aegypti Diptera: Culicidae larva were seen as the least sensitive hosts regarding their susceptibility to both entomopathogenic nematodes

Keywords

Entomopathogen nematodes, Steinernema feltiae, Heterorhabditis bacteriophora

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