Glucose 6-Phosphate Dehydrogenase Activatory Properties of Some Organic Compounds and Amino Acids

Year 2011, Volume: 39 Issue: 2, 157 - 162, 01.04.2011

Zafer Ömer Özdemir , Murat Şentürk Deniz Ekinci

Abstract

The aim of this study was to assess activatory potentials of 4’- Methylsulfonyl - acetophenone, 4- methylsulfonyl phenylacetic acid, tyrosine and phenylalanine on human erythrocyte glucose 6-phosphate dehydrogenase. For this purpose, initially erythrocyte glucose 6-phosphate dehydrogenase was managed to be purified 812-fold in a yield of 40.6% by using 2’,5’-ADP Sepharose 4B affinity gel. 4’- Methylsulfonyl acetophenone, 4- methylsulfonyl phenylacetic acid, tyrosine and phenylalanine exhibited activatory effects on the enzyme. % Activity values for these compounds were determined by plotting activity percentage versus [Activator].

Keywords

Activator, amino acid, enzyme, glucose 6-phosphate dehydrogenase

Bazı Organik Bileşikler ve Amino Asitlerin Glukoz 6-Fosfat Dehidrogenaz Aktivatör Özellikleri

Abstract

Bu çalışmanın amacı 4- metilsülfonil -asetofenon, 4- metilsülfonil fenilasetik asit, tirozin ve fenilalaninin insan eritrosit glukoz 6-fosfat dehidrogenaz üzerindeki aktivatör potansiyelini değerlendirmektir. Bu amaçla, öncelikle eritrosit glukoz 6-fosfat dehidrogenaz 2’,5’-ADP Sepharose 4B afinite jeli kullanılarak 812-kat, %40.6 verimle elde edildi. 4- Metilsülfonil fenilasetik asit, tirozin ve fenilalaninin enzim üzerinde aktivatör etkileri gösterdi. Bu bileşikler için % aktivite-[Aktivatör] grafiği kullanılarak % aktivite değerleri belirlendi.

Keywords

Aktivatör, amino asit, enzim, glukoz 6-fosfat dehidrogenaz

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