Research Article
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COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS

Year 2023, Volume: 51 Issue: 4, 327 - 340, 07.11.2023
https://doi.org/10.15671/hjbc.1245678

Abstract

Gliosis is a condition that plays a negative role in various neurological pathologies. In this study, it was aimed to obtain and compare the glio-protective effects of electrospun fibrous scaffolds coated by different biopolymers. First, in vitro gliosis model was obtained by inducing U-87 MG cells with LPS and IFN-γ. The optimized model was applied to U-87 MG cells grown on polycaprolactone (PCL), hyaluronic acid (HA) coated, gelatin coated, and collagen coated PCL scaffolds. Immunofluorescent (IF) staining was performed with glial fibrillary acidic protein (GFAP) antibody to determine the level of gliosis. It was found that 5µg/mL LPS concentration induced gliosis and hyaluronic acid-coated PCL scaffolds have shown a protective effect on gliosis.

Thanks

We would like to thank BUGAMED BIOTECHNOLOGY for providing hyaluronic acid as a gift. Also, we would like to thank Prof. Dr. Ayse NALBANTSOY for her support in cell culture experiments.

References

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Year 2023, Volume: 51 Issue: 4, 327 - 340, 07.11.2023
https://doi.org/10.15671/hjbc.1245678

Abstract

References

  • R. Huang, Y. Zhang, B. Han, Y. Bai, R. Zhou, G. Gan, J. Chao, H. Yao, Circular RNA HIPK2 regulates astrocyte activation via cooperation of autophagy and ER stress by targeting MIR124-2HG, Autophagy, 13 (2017).
  • Y. Hu, H. Zhang, H. Wei, H. Cheng, J. Cai, X. Chen, L. Xia, H. Wang, R. Chai, Scaffolds with anisotropic structure for neural tissue engineering, Engineered Regeneration, 3 (2022) 154–162.
  • R.A.L. De Sousa, Reactive gliosis in Alzheimer’s disease: a crucial role for cognitive impairment and memory loss, Metab Brain Dis, 37 (2022) 851–857.
  • M. Motavaf, • Majid Sadeghizadeh, • Mohammad Javan, Attempts to Overcome Remyelination Failure: Toward Opening New Therapeutic Avenues for Multiple Sclerosis, Cell Mol Neurobiol, 37 (n.d.).
  • S.C. Yetis, D.A. Ekinci, E. Cakir, E.M. Eksioglu, U.E. Ayten, A. Capar, B.U. Toreyin, B.E. Kerman, Myelin segmentation in fluorescence microscopy images, TIPTEKNO 2019 - Tip Teknolojileri Kongresi, (2019).
  • H. Wang, G. Song, H. Chuang, C. Chiu, A. Abdelmaksoud, Y. Ye, L. Zhao, Portrait of glial scar in neurological diseases, Int J Immunopathol Pharmacol, 31 (2018) 1–6.
  • Y. Wang, H. Tan, X. Hui, Biomaterial Scaffolds in Regenerative Therapy of the Central Nervous System, (2018).
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  • H. Yin, T. Jiang, X. Deng, M. Yu, H. Xing, X. Ren, A cellular spinal cord scaffold seeded with rat adipose-derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats, Mol Med Rep, 17 (2018) 2998.
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  • D. Doç Nimet Karagülle Mersin, Doku Mühendisliği Uygulamalari İçin Polivinil Alkol (Pva)/Nişasta Temelli Kriyojel Doku İskelelerinin Geliştirilmesi: Sentez, Karakterizasyon Ve Biyouyumluluk Değerlendirmeleri Doktora Tezi Seda Ceylan Mersin Üniversitesi Fen Bilimleri Enstitüsü Kimya Mühendisliği Anabilim Dali, N.D.
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  • F. Zamboni, M. Keays, S. Hayes, A.B. Albadarin, G.M. Walker, P.A. Kiely, M.N. Collins, Enhanced cell viability in hyaluronic acid coated poly(lactic-co-glycolic acid) porous scaffolds within microfluidic channels, Int J Pharm, 532 (2017) 595–602.
  • X. Lin, W. Wang, W. Zhang, Z. Zhang, G. Zhou, Y. Cao, W. Liu, Hyaluronic Acid Coating Enhances Biocompatibility of Nonwoven PGA Scaffold and Cartilage Formation, Https://Home.Liebertpub.Com/Tec, 23 (2017) 86–97.
  • I. Özaydin, E. Ünsaldi, Ö. Aksoy, S. Yayla, M. Kaya, M.B. Ulkay Tunali, A. Aktaş, E. Taşdemiroǧlu, M. Cihan, B. Kurt, H.C. Yildirim, A. Şengöz, H. Erdoǧan, Deneysel peri ve epinöral nörorafi uygulanmi{dotless}ş rat modellerinde silikon tüp ve silikon tüp + hyaluronik asit uygulamasi{dotless}ni{dotless}n adezyon formasyonuna etkisi, Kafkas Univ Vet Fak Derg, 20 (2014) 591–597.
  • X. Zhang, W. Qu, D. Li, K. Shi, R. Li, Y. Han, E. Jin, J. Ding, X. Chen, Functional Polymer-Based Nerve Guide Conduits to Promote Peripheral Nerve Regeneration, Adv Mater Interfaces, 7 (2020).
  • J. Cao, C. Sun, H. Zhao, Z. Xiao, B. Chen, J. Gao, T. Zheng, W. Wu, S. Wu, J. Wang, J. Dai, The use of laminin modified linear ordered collagen scaffolds loaded with laminin-binding ciliary neurotrophic factor for sciatic nerve regeneration in rats, Biomaterials, 32 (2011) 3939–3948.
  • G.K. Saraogi, P. Gupta, U.D. Gupta, N.K. Jain, G.P. Agrawal, Gelatin nanocarriers as potential vectors for effective management of tuberculosis, Int J Pharm, 385 (2010) 143–149.
  • M.A. Alvarez-Perez, V. Guarino, V. Cirillo, L. Ambrosio, Influence of gelatin cues in PCL electrospun membranes on nerve outgrowth, Biomacromolecules, 11 (2010) 2238–2246.
  • A. Kriebel, D. Hodde, T. Kuenzel, J. Engels, G. Brook, J. Mey, Cell-free artificial implants of electrospun fibres in a three-dimensional gelatin matrix support sciatic nerve regeneration in vivo, J Tissue Eng Regen Med, 11 (2017) 3289–3304.
  • M. Naseri-Nosar, S. Farzamfar, H. Sahrapeyma, S. Ghorbani, F. Bastami, A. Vaez, M. Salehi, Cerium oxide nanoparticle-containing poly (ε-caprolactone)/gelatin electrospun film as a potential wound dressing material: In vitro and in vivo evaluation, Materials Science and Engineering C, 81 (2017) 366–372.
  • F.K. Mediesse, T. Boudjeko, A. Hasitha, M. Gangadhar, W.F. Mbacham, P. Yogeeswari, Inhibition of lipopolysaccharide (LPS)-induced neuroinflammatory response by polysaccharide fractions of Khaya grandifoliola (CDC) stem bark, Cryptolepis sanguinolenta (Lindl) Schltr and Cymbopogon citratus Stapf leaves in raw 2647 macrophages and U87 glioblastoma cells, BMC Complement Altern Med, 18 (2018).
  • M. Potokar, M. Morita, G. Wiche, J. Jorgačevski, cells The Diversity of Intermediate Filaments in Astrocytes, (n.d.).
  • R. Helbok, R. Beer, Cerebrospinal fluid and brain extracellular fluid in severe brain trauma, Handb Clin Neurol, 146 (2018) 237–258.
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  • T. Fath, D. Lecca, G. Pacheco-Lopez, A. Vargas-Caraveo, D.M. Hermann, M. Sardari, E. Dzyubenko, B. Schmermund, D. Yin, Y. Qi, C. Kleinschnitz, in collaboration with reviewer GP-L Dose-Dependent Microglial and Astrocytic Responses Associated With Post-ischemic Neuroprotection After Lipopolysaccharide-Induced Sepsis-Like State in Mice, (2020).
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  • V.W. Yong, R. Moumdjian, F.P. Yong, T.C.G. Ruijs, M.S. Freedman, N. Cashman, J.P. Antel, yInterferon promotes proliferation of adult human astrocytes in vitro and reactive gliosis in the adult mouse brain in vivo, 1991.
  • A. Haque, A. Das, L.M. Hajiaghamohseni, A. Younger, N.L. Banik, S.K. Ray, Induction of apoptosis and immune response by all-trans retinoic acid plus interferon-gamma in human malignant glioblastoma T98G and U87MG cells, Cancer Immunology, Immunotherapy, 56 (2007) 615–625.
  • Ş. Çoğan, Sıçan Kemik İliğinden Ve Yağ Dokusundan Elde Edilen Mezenkimal Kök Hücrelerin Pcl Nanofibröz Yüzeyler Üzerinde Tutunma Ve Çoğalma Özelliklerinin İncelenmesi, Ege Üniversitesi Fen Bilimleri Enstitüsü  , n.d.
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  • F. Bonnier, M.E. Keating, T.P. Wróbel, K. Majzner, M. Baranska, A. Garcia-Munoz, A. Blanco, H.J. Byrne, Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models, Toxicology in Vitro, 29 (2015) 124–131.
  • S. Karmakar, N.L. Banik, S.K. Ray, Curcumin suppressed anti-apoptotic signals and activated cysteine proteases for apoptosis in human malignant glioblastoma U87MG cells, Neurochem Res, 32 (2007) 2103–2113.
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There are 52 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Research Article
Authors

Ece Battaloğlu 0000-0003-4761-1047

Zehra Gül Morçimen 0000-0001-8954-9996

Aylin Şendemir 0000-0003-1818-6651

Publication Date November 7, 2023
Acceptance Date June 23, 2023
Published in Issue Year 2023 Volume: 51 Issue: 4

Cite

APA Battaloğlu, E., Morçimen, Z. G., & Şendemir, A. (2023). COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS. Hacettepe Journal of Biology and Chemistry, 51(4), 327-340. https://doi.org/10.15671/hjbc.1245678
AMA Battaloğlu E, Morçimen ZG, Şendemir A. COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS. HJBC. November 2023;51(4):327-340. doi:10.15671/hjbc.1245678
Chicago Battaloğlu, Ece, Zehra Gül Morçimen, and Aylin Şendemir. “COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS”. Hacettepe Journal of Biology and Chemistry 51, no. 4 (November 2023): 327-40. https://doi.org/10.15671/hjbc.1245678.
EndNote Battaloğlu E, Morçimen ZG, Şendemir A (November 1, 2023) COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS. Hacettepe Journal of Biology and Chemistry 51 4 327–340.
IEEE E. Battaloğlu, Z. G. Morçimen, and A. Şendemir, “COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS”, HJBC, vol. 51, no. 4, pp. 327–340, 2023, doi: 10.15671/hjbc.1245678.
ISNAD Battaloğlu, Ece et al. “COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS”. Hacettepe Journal of Biology and Chemistry 51/4 (November 2023), 327-340. https://doi.org/10.15671/hjbc.1245678.
JAMA Battaloğlu E, Morçimen ZG, Şendemir A. COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS. HJBC. 2023;51:327–340.
MLA Battaloğlu, Ece et al. “COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS”. Hacettepe Journal of Biology and Chemistry, vol. 51, no. 4, 2023, pp. 327-40, doi:10.15671/hjbc.1245678.
Vancouver Battaloğlu E, Morçimen ZG, Şendemir A. COMPARISION OF THE GLIO-PROTECTIVE EFFECTS OF BIOPOLYMER COATED ELECTROSPUN SCAFFOLDS. HJBC. 2023;51(4):327-40.

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