Research Article
PDF Zotero Mendeley EndNote BibTex Cite

Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants

Year 2021, Volume 8, Issue 4, 339 - 345, 31.12.2021
https://doi.org/10.17350/HJSE19030000247

Abstract

Environmental pollution occurs in nature as air, soil and water pollution and as a result it affects whole ecosystem including human beings. Although industrialization and technological developments have made life easier than before, in recent years, they have triggered environmental pollution. Cadmium, which is a toxic pollutant for all living things, is one of the most important element in heavy metal pollutants. In this study, it was aimed to determine gene expression changes in tomato plant under Cd stress. Molecular response of tomato plants to Cd stress was examined by transcript accumulation analysis of two stress-related genes: (i) MT-2 (metallothionine-2) gene encodes metal binding protein and (ii) The GR-1 (glutathione reductase-1) gene encodes the glutathione reductase enzyme and is a marker of the ROS scavenging mechanism. Expression differences in MT-2 and GR-1 genes in tomato seedlings exposed to cadmium stress at different concentrations ranging from 20 to 1280 mg L-1 for 24 hours were determined performing quantitative real-time PCR. The results obtained from this study were showed that MT-2 and GR-1 genes play an important role in the mechanism of protection against heavy metal of Cd stress. In addition, the physiological properties of tomato have been associated with cadmium accumulation.

References

  • Peralta I, Spooner D. History, origin and early cultivation of tomato (Solanaceae) (2006).
  • FAO. Food and agriculture organization of The United Nations Rome (2019) ISBN 978-92-5-106215-9.
  • Hall JL. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany 53 (2002) 1-11.
  • Alirzayeva EG, Shirvani TS, Yazici MA, Alverdiyeva S, Shukurov ES, Ozturk L, Ali-Zade VM, Cakmak I. Heavy metal accumulation in Artemisia and foliaceous lichen species from the Azerbaijan flora. Forest Snow and Landscape Research 80 (2006) 339-348.
  • Miwa K, Kamiya T, Fujiwara T. Homeostasis of the structurally important micronutrients, B and Si. Current Opinion in Plant Biology 12 (2009) 307-311.
  • Kirbag F, Munzuroglu O. Toxic effects of cadmium (Cd+2) on metabolism of sunflower (Helianthus annuus L.) seedlings. Acta. Agriculture. Scand, Section B-Soil and Plant Science 56 (2006) 224-229.
  • Bolukbasi E and Karakas M. Effect of zinc heavy metal on stress-related genes in tomato (Solanum lycopersicum L.) plants. International Journal of Environment Agriculture and Biotechnology 4 (2019) 1211-1216.
  • Cervilla LM, Blasco B, Rios J, Romero L, Ruiz J. Oxidative stress and antioxidants in tomato (Solanum lycopersicum) plants subjected to boron toxicity. Annual Botany 100 (2007) 747-756.
  • Chalapathi R, Reddy AR. Glutathione reductase: a putative redox regulatory system in plant cells. In: Khan, N.A. et al. (Eds.), Sulfur assimilation and abiotic stress in plants. Springer-Verlag, Berlin, Heidelberg (2008) 111-147.
  • Temel Y, Taysi MS. The Effect of Mercury Chloride and Boric Acid on Rat Erythrocyte Enzymes. Biological Trace Element Research 191 (2019) 177-182.
  • Temel Y, Kufrevioglu OI, Ciftci M. Investigation of the effects of purification and characterization of Turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. Turkish Journal of Chemistry 41 (2017) 48-60.
  • Chomczynski P, Mackey K. Short technical reports. Modification of the TRI reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources. Biotechniques 19 (1995) 942-945.
  • Conte C, Mutti I, Puglisi P, Ferrarini A, Regina GRG, Maestri E, Marmiroli N. DNA fingerprint analysis by PCR based method for monitoring the genotoxic effects of heavy metals pollution. Chemosphere 37 (1998) 2739-2749.
  • Temel Y, Bengu AS, Akkoyun HT, Akkoyun M, Ciftci M. Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats. Journal of biochemical and molecular toxicology, 31 (2017) 1-5.
  • Del Razo LM, Quintanilla-Vega B, Brambila-Colombres E, Caldero´ n-Aranda ES, Manno M, Albores A. Stress proteins induced by arsenic. Toxicology and Applied Pharmacology 177 (2001) 132-148.
  • Apel K, Hirt H. Reactive oxygen species: metabolism oxidative stress, and signal transduction. Annual Review of Plant Biology 55 (2004) 373-399.
  • DalCorso G, Farinati S, Maistri S, Furini A. How plants cope with cadmium: staking all on metabolism and gene expression. Journal of International Plant Biology 50 (2008) 1268-1280.
  • Waisberg MP, Joseph B, Beyersmann D. Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicology 192 (2003) 95-117.
  • Liu W, Yang YS, Zhou QX, Xie LJ, Li PJ, Sun TH. Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers. Chemosphere 67 (2007) 1155-1163.
  • Mirouze M, Paszkowski J. Epigenetic contribution to stress adaptation in plants. Current Opinion in Plant Biology 14 (2011) 267-274.
  • Bolukbasi E, Aras ES. Determination of DNA methylation levels with CRED-RA technique in the genome of sunflower seedlings (Helianthus annuus L.) subjected to zinc stress. International Journal of Environment, Agriculture and Biotechnology 3 (2016) 438-444.
  • Goupila P, Souguira D, Ferjanib E, Faurec O, Hitmid A, Ledoigta G. Expression of stress-related genes in tomato plants exposed to arsenic and chromium in nutrient solution. Journal of Plant Physiology 166 (2009) 1446-1452.
  • Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48 (2010) 909-930.
  • Gebretsadkan G. Evaluating the effect of integrated use of farm yard manure and urea on the socio-economic performance of tomato (Lycopersicum esculentum Mill) at Tselemti Woreda, North western Tigray, Ethiopia. International Journal of Environment, Agriculture and Biotechnology 3 (2018) 748-755.
  • Kosnett MJ. Heavy metal intoxication and chelators. In: katzung BG(ed) Basic and clinical pharmacology. 10th edn. McGraw-Hill, New York (2007) 945-957.
  • Wang L, Yang L, Yang F, Li X, Song Y, Wang X, Hu X. Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity. The Journal of Plant Physiology 167 (2010) 1298-1306.
  • Tombuloglu H, Semizoglu N, Sakcali S, Kekec G. Boron induced expression of some stress-related genes in tomato. Chemosphere 86 (2012) 433-438.
  • Temel Y, Taher SSM, Hamza MA, Shafeeq IH, Kocyigit UM, Ciftci M. Investigation of the inhibition effects of some metal ions on glutathione reductase enzyme from japanese quail (Coturnix coturnix japonica) liver. Cumhuriyet Science Journal 39 (2018) 679-687.
  • Ziller A, Fraissinet-Tachet L. Metallothionein diversity and distribution in the tree of life: a multifunctional protein. Metallomics 10 (2018) 1549-1559.
  • Temel Y, Bozkus T, Ciftci M. Glutatyon redüktaz (GR) enziminin japon bıldırcın (Coturnix coturnix japanica) eritrositlerinden saflaştırılması ve karakterizasyonu. Journal of the Institute of Science and Technology 7 (2017) 143-150.
  • Rono JK, Le Wang L, Wu XC, Cao HW, Zhao YN. Identification of a new function of metallothionein-like gene OsMT1e for cadmium detoxification and potential phytoremediation. Chemosphere 265 (2021) 129-136.
  • Bolukbasi E, Aras ES. Determination of physiological biochemical and molecular effects of zinc stress on the growth of sunflower seedlings (Helianthus annuus L.). International Journal of Environment, Agriculture and Biotechnology 3 (2018) 530-536.
  • Wang Q, Pu Y, Yang D, Yin X, He Z. Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea. Biochemical and Biophysical Research Communications 495 (2018) 1851-1857.
  • Creissen GP, Edwards EA, Mullineaux PM. Glutathione reductase and ascorbate peroxidase. In Causes of photooxidative stress and amelioration of defense systems in plants CRC press (2019) 343-364.
  • Hasanuzzaman M, Bhuyan MHM, Anee TI, Parvin K, Nahar K. Regulation of ascorbate-glutathione pathway in mitigating oxidative damage in plants under abiotic stress. Antioxidants 8 (2019) 380-384.
  • Rozen S, Skaletsky H. Primer-3 on the www for general users and for biologist programmers. Methods in Molecular Biology 132 (2000) 365-386.
  • Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25 (2001) 402-408.

Year 2021, Volume 8, Issue 4, 339 - 345, 31.12.2021
https://doi.org/10.17350/HJSE19030000247

Abstract

References

  • Peralta I, Spooner D. History, origin and early cultivation of tomato (Solanaceae) (2006).
  • FAO. Food and agriculture organization of The United Nations Rome (2019) ISBN 978-92-5-106215-9.
  • Hall JL. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany 53 (2002) 1-11.
  • Alirzayeva EG, Shirvani TS, Yazici MA, Alverdiyeva S, Shukurov ES, Ozturk L, Ali-Zade VM, Cakmak I. Heavy metal accumulation in Artemisia and foliaceous lichen species from the Azerbaijan flora. Forest Snow and Landscape Research 80 (2006) 339-348.
  • Miwa K, Kamiya T, Fujiwara T. Homeostasis of the structurally important micronutrients, B and Si. Current Opinion in Plant Biology 12 (2009) 307-311.
  • Kirbag F, Munzuroglu O. Toxic effects of cadmium (Cd+2) on metabolism of sunflower (Helianthus annuus L.) seedlings. Acta. Agriculture. Scand, Section B-Soil and Plant Science 56 (2006) 224-229.
  • Bolukbasi E and Karakas M. Effect of zinc heavy metal on stress-related genes in tomato (Solanum lycopersicum L.) plants. International Journal of Environment Agriculture and Biotechnology 4 (2019) 1211-1216.
  • Cervilla LM, Blasco B, Rios J, Romero L, Ruiz J. Oxidative stress and antioxidants in tomato (Solanum lycopersicum) plants subjected to boron toxicity. Annual Botany 100 (2007) 747-756.
  • Chalapathi R, Reddy AR. Glutathione reductase: a putative redox regulatory system in plant cells. In: Khan, N.A. et al. (Eds.), Sulfur assimilation and abiotic stress in plants. Springer-Verlag, Berlin, Heidelberg (2008) 111-147.
  • Temel Y, Taysi MS. The Effect of Mercury Chloride and Boric Acid on Rat Erythrocyte Enzymes. Biological Trace Element Research 191 (2019) 177-182.
  • Temel Y, Kufrevioglu OI, Ciftci M. Investigation of the effects of purification and characterization of Turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. Turkish Journal of Chemistry 41 (2017) 48-60.
  • Chomczynski P, Mackey K. Short technical reports. Modification of the TRI reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources. Biotechniques 19 (1995) 942-945.
  • Conte C, Mutti I, Puglisi P, Ferrarini A, Regina GRG, Maestri E, Marmiroli N. DNA fingerprint analysis by PCR based method for monitoring the genotoxic effects of heavy metals pollution. Chemosphere 37 (1998) 2739-2749.
  • Temel Y, Bengu AS, Akkoyun HT, Akkoyun M, Ciftci M. Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats. Journal of biochemical and molecular toxicology, 31 (2017) 1-5.
  • Del Razo LM, Quintanilla-Vega B, Brambila-Colombres E, Caldero´ n-Aranda ES, Manno M, Albores A. Stress proteins induced by arsenic. Toxicology and Applied Pharmacology 177 (2001) 132-148.
  • Apel K, Hirt H. Reactive oxygen species: metabolism oxidative stress, and signal transduction. Annual Review of Plant Biology 55 (2004) 373-399.
  • DalCorso G, Farinati S, Maistri S, Furini A. How plants cope with cadmium: staking all on metabolism and gene expression. Journal of International Plant Biology 50 (2008) 1268-1280.
  • Waisberg MP, Joseph B, Beyersmann D. Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicology 192 (2003) 95-117.
  • Liu W, Yang YS, Zhou QX, Xie LJ, Li PJ, Sun TH. Impact assessment of cadmium contamination on rice (Oryza sativa L.) seedlings at molecular and population levels using multiple biomarkers. Chemosphere 67 (2007) 1155-1163.
  • Mirouze M, Paszkowski J. Epigenetic contribution to stress adaptation in plants. Current Opinion in Plant Biology 14 (2011) 267-274.
  • Bolukbasi E, Aras ES. Determination of DNA methylation levels with CRED-RA technique in the genome of sunflower seedlings (Helianthus annuus L.) subjected to zinc stress. International Journal of Environment, Agriculture and Biotechnology 3 (2016) 438-444.
  • Goupila P, Souguira D, Ferjanib E, Faurec O, Hitmid A, Ledoigta G. Expression of stress-related genes in tomato plants exposed to arsenic and chromium in nutrient solution. Journal of Plant Physiology 166 (2009) 1446-1452.
  • Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48 (2010) 909-930.
  • Gebretsadkan G. Evaluating the effect of integrated use of farm yard manure and urea on the socio-economic performance of tomato (Lycopersicum esculentum Mill) at Tselemti Woreda, North western Tigray, Ethiopia. International Journal of Environment, Agriculture and Biotechnology 3 (2018) 748-755.
  • Kosnett MJ. Heavy metal intoxication and chelators. In: katzung BG(ed) Basic and clinical pharmacology. 10th edn. McGraw-Hill, New York (2007) 945-957.
  • Wang L, Yang L, Yang F, Li X, Song Y, Wang X, Hu X. Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity. The Journal of Plant Physiology 167 (2010) 1298-1306.
  • Tombuloglu H, Semizoglu N, Sakcali S, Kekec G. Boron induced expression of some stress-related genes in tomato. Chemosphere 86 (2012) 433-438.
  • Temel Y, Taher SSM, Hamza MA, Shafeeq IH, Kocyigit UM, Ciftci M. Investigation of the inhibition effects of some metal ions on glutathione reductase enzyme from japanese quail (Coturnix coturnix japonica) liver. Cumhuriyet Science Journal 39 (2018) 679-687.
  • Ziller A, Fraissinet-Tachet L. Metallothionein diversity and distribution in the tree of life: a multifunctional protein. Metallomics 10 (2018) 1549-1559.
  • Temel Y, Bozkus T, Ciftci M. Glutatyon redüktaz (GR) enziminin japon bıldırcın (Coturnix coturnix japanica) eritrositlerinden saflaştırılması ve karakterizasyonu. Journal of the Institute of Science and Technology 7 (2017) 143-150.
  • Rono JK, Le Wang L, Wu XC, Cao HW, Zhao YN. Identification of a new function of metallothionein-like gene OsMT1e for cadmium detoxification and potential phytoremediation. Chemosphere 265 (2021) 129-136.
  • Bolukbasi E, Aras ES. Determination of physiological biochemical and molecular effects of zinc stress on the growth of sunflower seedlings (Helianthus annuus L.). International Journal of Environment, Agriculture and Biotechnology 3 (2018) 530-536.
  • Wang Q, Pu Y, Yang D, Yin X, He Z. Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea. Biochemical and Biophysical Research Communications 495 (2018) 1851-1857.
  • Creissen GP, Edwards EA, Mullineaux PM. Glutathione reductase and ascorbate peroxidase. In Causes of photooxidative stress and amelioration of defense systems in plants CRC press (2019) 343-364.
  • Hasanuzzaman M, Bhuyan MHM, Anee TI, Parvin K, Nahar K. Regulation of ascorbate-glutathione pathway in mitigating oxidative damage in plants under abiotic stress. Antioxidants 8 (2019) 380-384.
  • Rozen S, Skaletsky H. Primer-3 on the www for general users and for biologist programmers. Methods in Molecular Biology 132 (2000) 365-386.
  • Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25 (2001) 402-408.

Details

Primary Language English
Subjects Basic Sciences
Journal Section Research Articles
Authors

Ekrem BÖLÜKBAŞI (Primary Author)
AMASYA ÜNİVERSİTESİ
0000-0003-3828-1226
Türkiye

Supporting Institution Amasya University Scientific Research Unit
Project Number FMB-BAP 20-0480
Thanks The author gratefully acknowledge the financial support of this work by Amasya University Scientific Research Unit (FMB-BAP 20-0480). And also, the author acknowledges to Prof. Dr. Emine Sumer ARAS at Ankara University, Science Faculty, and Department of Biology for her valuable support.
Publication Date December 31, 2021
Application Date November 11, 2021
Acceptance Date December 20, 2021
Published in Issue Year 2021, Volume 8, Issue 4

Cite

Bibtex @research article { hjse1022458, journal = {Hittite Journal of Science and Engineering}, issn = {}, eissn = {2148-4171}, address = {Hitit Üniversitesi Mühendislik Fakültesi Kuzey Kampüsü Çevre Yolu Bulvarı 19030 Çorum / TÜRKİYE}, publisher = {Hitit University}, year = {2021}, volume = {8}, pages = {339 - 345}, doi = {10.17350/HJSE19030000247}, title = {Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants}, key = {cite}, author = {Bölükbaşı, Ekrem} }
APA Bölükbaşı, E. (2021). Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants . Hittite Journal of Science and Engineering , 8 (4) , 339-345 . DOI: 10.17350/HJSE19030000247
MLA Bölükbaşı, E. "Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants" . Hittite Journal of Science and Engineering 8 (2021 ): 339-345 <https://dergipark.org.tr/en/pub/hjse/issue/67751/1022458>
Chicago Bölükbaşı, E. "Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants". Hittite Journal of Science and Engineering 8 (2021 ): 339-345
RIS TY - JOUR T1 - Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants AU - Ekrem Bölükbaşı Y1 - 2021 PY - 2021 N1 - doi: 10.17350/HJSE19030000247 DO - 10.17350/HJSE19030000247 T2 - Hittite Journal of Science and Engineering JF - Journal JO - JOR SP - 339 EP - 345 VL - 8 IS - 4 SN - -2148-4171 M3 - doi: 10.17350/HJSE19030000247 UR - https://doi.org/10.17350/HJSE19030000247 Y2 - 2021 ER -
EndNote %0 Hittite Journal of Science and Engineering Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants %A Ekrem Bölükbaşı %T Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants %D 2021 %J Hittite Journal of Science and Engineering %P -2148-4171 %V 8 %N 4 %R doi: 10.17350/HJSE19030000247 %U 10.17350/HJSE19030000247
ISNAD Bölükbaşı, Ekrem . "Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants". Hittite Journal of Science and Engineering 8 / 4 (December 2021): 339-345 . https://doi.org/10.17350/HJSE19030000247
AMA Bölükbaşı E. Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants. Hittite J Sci Eng. 2021; 8(4): 339-345.
Vancouver Bölükbaşı E. Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants. Hittite Journal of Science and Engineering. 2021; 8(4): 339-345.
IEEE E. Bölükbaşı , "Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants", Hittite Journal of Science and Engineering, vol. 8, no. 4, pp. 339-345, Dec. 2021, doi:10.17350/HJSE19030000247