Use of Heat-killed Aspergillus ochraceus NRRL 3174 Discs as Biosorbent for petroleum Removal

Year 2023, Volume: 10 Issue: 3, 219 - 228, 30.09.2023

Nermin Hande Avcioglu Sezen Bılen Ozyurek Işıl Seyis Bilkay

https://doi.org/10.17350/HJSE19030000310

Abstract

The purpose of this work was to evaluate the petroleum sorption capacity of heat-killed fungal discs obtained from Aspergillus ochraceus strain. Effect of various parameters such as biosorbent dose (0.5g-2.5g/100mL), petroleum concentration (0.5-5%), pH (4.0-8.0), contact time (1-12h) and re-usability of biosorbent (1-6) were investigated. Accordingly, the highest biosorption capacity was obtained with 1% petroleum concentration, 1.5 g/100mL heat-killed fungal discs, 10h contact time at pH: 5.0 and room temperature. Additionally, each disc was able to actively use for at least 6 more cycles in biosorption experiments. The specific removal rate was calculated as 0.114 day−1, the rate constant and half-life period were also 1.609 day-1, t1/2 = 0.431, respectively. The kinetic study was described by the pseudo-second order model and the equilibrium modeling was found to be well fitted with Langmuir isotherm. The biosorbent(s) were characterized by Focused Ion Beam Scanning Electron Microscopy (FIB-SEM). Over 80% removal of long-chain n-alkanes by the heat-killed fungal discs was confirmed by GC-MS analysis. Since there has been no similar study investigating the sorption of petroleum with heat-killed Aspergillus ochraceous discs, this novel bio-based sorbent with its low cost, environmentally friendly and easy-to-apply properties can be used in advanced biosorption studies.

Keywords

Petroleum, Biosorption, Heat-killed Aspergillus ochraceus, Biosorption Isotherm and Kinetics

Supporting Institution

Hacettepe University

Project Number

FHD-2019-17719

Thanks

This work is funded by Hacettepe University Scientific Research Projects Coordination Unit (Project number: FHD-2019-17719).

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