hortis Horticultural Studies 2717-882X Antalya Batı Akdeniz Agricultural Research Institute 10.16882/hortis.1069414 Agricultural Engineering Ziraat Mühendisliği Determination of The Response of Wild and Cultivated Tomato Genotypes to Some Disease and Pests by Molecular Markers Aktaş Hakan ISPARTA UNIVERSITY OF APPLIED SCIENCES https://orcid.org/0000-0002-5612-6812 Aydın Gülnur ALANYA ALAADDIN KEYKUBAT UNIVERSITY 04 06 2022 39 1 15 21 12 21 2021 01 04 2022 Copyright © 2020, Horticultural Studies 2020 Horticultural Studies

Fusarium oxysporum f. sp. lycopersici, Verticillium spp., and nematodes, as well as virus diseases that negatively affect production with limited chemical control cause significant losses in greenhouse tomato cultivation. The practical and effective side of controlling diseases is genetic control by breeding. Using a genomic approach for plant breeding is a more sustainable and effective way to control disease and pests. The development of the resistant line is improved by conventional breeding methods that can be conducted over a long period. However, molecular markers make these processes considerably shorter with identifying resistant individuals. In this study, 14 wild and 188 cultivated tomato genotypes have been tested against Fusarium oxysporum f. sp. radicis-lycopersici (FORL), Meloidogyne spp. (root-knot nematodes), Tomato spotted wilt virus (TSWV), Tomato mosaic virus (ToMV), Tomato yellow leaf curl virus (TYLCV) and Verticillum dahliae Kleb. (Ve) diseases and pests with using MAS (Marker- Assisted Selection) technique. According to these results, it has been determined that the selected markers can be used effectively in breeding studies to determine the diseases mentioned above.

 Disease resistant MAS Molecular markers Tomato Acciarri, N., Rotino, G.L., Tamietti G., Valentino, D., Voltattorni, S., & Sabatini E. (2007). Molecular markers for Ve1 and Ve2: Verticillium resistance genes from Italian tomato germplasm. Plant Breeding, 126:617-621. Agrama, H.A., & Scott, J.W. (2006). Quantitative trait loci for Tomato Yellow Leaf Curl Virus and Tomato Mottle Virus resistance in tomato. Journal of the American Society for Horticultural Science, 131:267-272. Anbinder, I., Reuveni, M., Azari, R., Paran, I., Nahon, S., Shlomo, H., & Levin, I. (2009). Molecular dissection of Tomato Leaf Curl Virus resistance in tomato line TY172 derived from Solanum peruvianum. Theoretical and Applied Genetics, 119:519-530. Çevik, B., & Erkıș, G. (2008). First report of Tomato chlorosis virus in Turkey. Plant Pathology, 57(4). Dax, E., Livneh, O., Aliskevicius, E., Edelbaum, O., Kedar, N., Gavish, N., & Sela, I. (1998). A SCAR marker linked to the ToMV resistance gene, Tm2 2, in tomato. Euphytica, 101:73-77. De Castro, A.P., Blanca, J.M., Díez, M.J., & Viñals, F.N. (2007). Identification of a CAPS marker tightly linked to the Tomato Yellow Leaf Curl Disease resistance gene Ty-1 in tomato. European Journal of Plant Pathology, 117:347-356. Devran, Z., & Elekçioğlu, İ.H. (2004). The screening of F2 plants for the Root-Knot Nematode resistance gene, Mi by PCR in tomato. Turkish Journal of Agriculture and Forestry, 28:253-257. Dianese, E.C., Fonseca, M.E. Goldbach, R., Kormelink, R., Inoue-Nagata, A.K. Resende, R.O., & Boiteux, L.S. (2010). Development of a locus-specific, co-dominant SCAR marker for assisted-selection of the Sw-5 (Tospovirus resistance) gene cluster in a wide range of tomato accessions. Molecular Breeding, 25:113-142. Doyle, J.J., & Doyle, J. (1987). A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochemical bulletin, 19:11-15. El Mehrach, K., Gharsallah Chouchane, S., Mejia, L., Williamson, V. M., Vidavski, F., Hatimi, A., & Maxwell, D.P. (2005). PCR-based methods for tagging the Mi-1 locus for resistance to Root-Knot Nematode in Begomovirus-resistant tomato germplasm. Acta Horticulturae, 695:263-270. Fidan, H., & Sarı, N. (2019). Phenotypic characterization of the isolate that breaks resistance to Tomato Spotted Wilt Virus in tomato. Mediterranean Agricultural Sciences, 32:307-314. Fidan, H. (2020). Tomato Brown Rugose Fruit Virus (ToBRFV): current situation and future prospects. Mediterranean Agricultural Sciences, 33:43-49. Foolad, M.R., & Sharma, A. (2004). Molecular markers as selection tools in tomato breeding. Acta Horticulturae, 695:225-240. Foolad, M.R. (2007). Genome mapping and molecular breeding of tomato. International Journal of Plant Genomics. 2007:1-52. Gebhardt, C. (2007). Molecular markers, maps and population genetics. In Potato Biology and Biotechnology, Elsevier Science B.V. p. 77-89. Grube, R.C., Radwanski, E.R., & Jahn, M. (2000). Comparative genetics of disease resistance within the solanaceae. Genetics, 155:873–887. Guldur, M.E., Ozaslan, M., Baloglu, S., & Yilmaz, M. A. (1994). Pepper mild mottle virus in pepper in Turkey. In Proceedings of the 9th Congress of the Mediterranean Phytopathological Union, Kusadası, pp. 465-467. Hall, T.J. (1980). Resistance at the Tm-2 locus in the tomato to tomato mosaic virus. Euphytica, 29:189-197. Hanson, P., Lu, S.F., Wang, J.F., Chen, W., Kenyon, L., Tan, C.W., & Yang, R.Y. (2016). Conventional and molecular marker-assisted selection and pyramiding of genes for multiple disease resistance in tomato. Scientia Horticulturae, 201:346-354. Ji, Y., Schuster, D.J., & Scott, J.W. (2007a). Ty-3, a Begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato. Molecular Breeding, 20:271-284. Ji, Y., Scott, J.W., Hanson, P., Graham, E. & Maxwell, D.P. (2007b). Sources of resistance, inheritance, and location of genetic loci conferring resistance to members of the tomato-infecting Begomoviruses. In Tomato Yellow Leaf Curl Virus disease. Springer, Dordrecht. p. 343-362. Kaur, S., Dhaliwal, M.S., Cheema, D.S., Jindal, S.K., & Gaikwad, A.K. (2014). Screening of tomato (Solanum lycopersicum L.) germplasm for Root-Knot Nematode resistance using conventional and molecular marker techniques. Indian Journal of Nematology, 44:56-61. Kawchuk, L.M, Hachey, J., Lynch, D.R., Kulcsar, F., van Rooijen, G., Waterer, D.R., Robertson, A., Kokko, E., Byers, R., Howard, R.J., Fischer, R., & Prüfer, D. (2001). Tomato Ve disease resistance genes encode cell surface-like receptors. PNAS, 98:6511–6515. Kaya, H.B., & Tanyolaç, B. (2009). Screening of F3 segregation population lines revealed by Ty-1 markers linked to resistance locus of Tomato Yellow Leaf Curl Disease (TYLCD) in tomato (Lycopersicum esculentum). International Journal of Natural and Engineering Sciences, 33:149-153. Kaya, Y., Al-Remi, F., Arvas, Y.E., & Durmus, M. (2018). Tomato plant and ıts ın vitro micropropagation. Journal of Engineering Technology and Applied and Applied Sciences, 3:57-73. Lanfermeijer, F.C., Dijkhuis, J., Sturre, M.J., de Haan, P., & Hille, J. (2003). Cloning and characterization of the durable Tomato Mosaic Virus resistance gene Tm-2 2 from Lycopersicon esculentum. Plant Molecular Biology, 52:1039-1051. Laterrot, H., & Moretti, A. (1991). Allelism of various FORL resistance sources. Report of the Tomato Genetics Cooperative, 41:28-30. Lee, H.J., Kim, B., Bae, C., Kang, W.H., Kang, B.C., Yeam, I., & Oh, C.S. (2015). Development of a single-nucleotide polymorphism marker for the Sw-5b gene conferring disease resistance to Tomato Spotted Wilt Virus in tomato. Horticultural Science & Technology, 33:730-736. Levesque, H., Vedel, F., Mathieu, C., & De Courcel, A.G.L. (1990). Identification of a short rDNA spacer sequence highly specific of a tomato line containing Tm-1 gene introgressed from Lycopersicon hirsutum. Theoretical and Applied Genetics, 80:602-608. Mason, G., Roggero, P., & Tavella, L. (2003). Detection of Tomato Spotted Wilt Virus in its vector Frankliniella occidentalis by reverse transcription polymerase chain reaction. Journal of Virological Methods, 109:69-73. Morid, B., & Haj Mansour, S. (2017). Assessment of tomato genotypes resistance to Verticillium and Fusarium wilt diseases using molecular markers. Journal of Microbial World, 10:80-93. Mutlu, N., Demirelli, A., Ilbi, H., & Ikten, C. (2015). Development of codominant SCAR markers linked to resistant gene against the Fusarium oxysporum f. sp. radicis-lycopersici. Theoretical and Applied Genetics, 128:1791-1798. Nascimento, I.R.D., Maluf, W.R., Figueira, A.R., Menezes, C.B., Resende, J.T.V.D., Faria, M.V., & Nogueira, D.W. (2009). Marker assisted identification of Tospovirus resistant tomato genotypes in segregating progenies. Scientia Agricola, 66:298-303. Nie, X., & Singh, R.P. (2002). A new approach for the simultaneous differentiation of biological and geographical strains of Potato Virus Y by uniplex and multiplex RT-PCR. Journal of Virological Methods, 104:41-54. Ohmori, T., Murata, M., & Motoyoshi, F. (1995). Identification of RAPD markers linked to the Tm-2 locus in tomato. Theoretical and Applied Genetics, 90:307-311. Ohmori, T., Murata, M., & Motoyoshi, F. (1996). Molecular Characterization of RAPD and SCAR Markers Linked to the Tm-1 Locus in Tomato, Theoretical and Applied Genetics, 92:151-156. Pelham, J. (1966). Resistance in tomato to Tobacco Mosaic Virus. Euphytica, 15:258-267. Roberts, P.D., McGovern, R.J., & Datnoff, L.E. (2000). Fusarium crown and root rot of tomato in Florida. Plant Pathology Fact Sheet, 184:1-4. Seah, S., Williamson, V.M., Garcia, B.E., Mejia, L., Salus, M.S., Martin, C.T. & Maxwell, D.P. (2007). Evaluation of a co-dominant SCAR marker for detection of the Mi-1 locus for resistance to Root-Knot Nematode in tomato germplasm. Tomato Genetic Cooperative Report, 57:37-40. Tanksley, S.D., Ganal, M.W., Prince, J.P., De Vicente, M.C., Bonierbale, M.W., Broun, P., & Martin, G. (1992). High density molecular linkage maps of the tomato and potato genomes. Genetics, 132:1141-1160. TGRC (2014). Tomato Genetics Resource Center, University of California, Davis, CA 95616. https://tgrc.ucdavis.edu/. Accessed date: 10 September, 2021. Yurtmen, M., Guldur, M.E. & Yilmaz, M.A. (1999). Tomato Spotted Wilt Virus on peppers in Icel Province of Turkey. Petria, 9:243-344. Zamir, D., Ekstein-Michelson, I., Zakay, Y., Navot, N., Zeidan, M., Sarfatti, M., & Czosnek, H. (1994). Mapping and introgression of a Tomato Yellow Leaf Curl Virus tolerance gene, Ty-1. Theoretical and Applied Genetics, 88:141-146. Zengin, S. & İlbi, H. (2016). Development of Tomato Lines Resistant to Viral (Tomato Yellow Leaf Curl Virus, Tomato Spotted Wilt Virus), Fungal (Root and Root Rot Rot), Diseases and Nematodes (Meloidogyne Incognita) by Molecular Marker Assisted Selection. PhD Thesis, Ege University, İzmir (in Turkish).