Investigation of In vitro Propagation Possibilities of Endemic Campanula phitosiana Yıldırım & Şentürk

Year 2022, Volume: 39 Issue: 3, 107 - 112, 15.12.2022

Ademi Fahri Pırhan , Ebru Sevindik Başar Sevindik

https://doi.org/10.16882/hortis.1200592

Cited By: 1

Abstract

Campanula phitosiana Yıldırım & Şentürk, is a local endemic to the Aydın Mountain range, which distribute across in Western Anatolia (both İzmir and Aydın). This species belongs to the Mediterranean basin floristic region. According to the International Union for Conservation of Nature’s criteria, C. phitosiana is classified as “Critically Endangered” (CR). The aim of the study is to develop in vitro regeneration protocol for critically endangered endemic Campanula phitosiana. To investigate the efficient medium and plant growth regulator combinations for callus initiation and shoot proliferation, petiole and leaves were used as explant and explants cultivated on MS medium including NAA (1-Naphthylacetic acid) (0.3 mg L-1), TDZ (Thidiazuron) (0.5, 1.0, 2.0, and 3.0 mg L-1), BA (6-Benzylaminopurine) (0.5, 1.0, 2.0, and 3.0 mg L-1) and Gibberellic Acid (GA3) (0.5, 1.0, 2.0, and 3.0 mg L-1). Explants subcultured 3 times. Experiments were conducted according to completely randomized design repeated with 5 replicates and each replicates including 5 explants. As a result of the experiment, callus initiation and shoot proliferation were investigated. Efficient callus initiation was observed petiole explants as 100% rate. Shoot proliferation was observed on MS medium including 0.3 mg L-1 NAA + 2.0 mg L-1 GA3.

Keywords

Campanula, Endemic, Plant growth regulator, Regeneration, Tissue calture

References

• Airo, M., Savona, M., Mascarello, C., De Benedetti, L., Nicoletti, F., Casazza, G., Ruffoni, B., & Minuto, L. (2009). Analysis of in vitro performances and genetic variability of several Campanula sabatia populations in Italy. In IV International Symposium on Breeding Research on Medicinal and Aromatic Plants-ISBMAP2009 860 (pp. 109-112).
• Alçıtepe, E., Everest, A., & Sungur, M.A. (2011). Some soil parameters in Campanula species (sect. Quinqueloculares) from Mediterranean climate areas in Turkey. African Journal of Agricultural Research, 6(7):1735–1743.
• Brandt, K. (1994). Variation among and within clones in formation of roots and shoots during micropropagation of Campanula isophylla. Plant Cell Tissue and Organ Culture, 39(1):63-68.
• Contandriopoulos, J. (1984). Differentiation and evolution of the genus Campanula in the Mediterranean region. In Grant, W.F. (Ed.). Plant biosystematics (ss 140–175). Ontario: Academic Press.
• Fedorov, A.A., & Kovanda, M. (1976). Campanula L. In Tutin, T.G., Heywood, V.H., Burges, N.A., & Valentine, D.H. (Eds.). Flora Europaea, vol. 4 (ss 74–93). London: Cambridge University Press.
• Frello, S., Stummann, B.M., & Serek, M. (2002). Shoot regeneration of Campanula carpatica Jacq. (Campanulaceae) via callus phase. Scientia Horticulturae, 93(1):85-90.
• Grigoriadou, K., Krigas, N., & Maloupa, E. (2014). GIS-facilitated ex situ conservation of the rare Greek endemic Campanula incurva Aucher: Seed germination requirements and effect of growth regulators on in vitro proliferation and rooting. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 148(6):1169-1177.
• Joung, Y.H., Liao, M.S., Roh, M.S., Kamo, K., & Song, J.S. (2002). In vitro propagation of Campanula glomerata, ‘Acaulis’ from leaf blade explants. Scientia Horticulturae, 92(2):137-146.
• Kolomiets, T.M., Malyarovskaya, V.I., & Samarina, L.S. (2016). In vitro conservation of Campanula sclerophylla Kolak endemic endangered species of Western Caucasus. Plant Tissue Culture and Biotechnology, 26(2):143-149.
• Lammers, T.G. (2007). Campanulaceae Jussieu, Gen. Pl. 163 (1789), nom. Cons. In Kadereit, J.W. & Jeffrey, C. (Eds.). The families and genera of vascular plants, Vol. VIII, Asterales (ss 26-57). Springer.
• Maria, A.E. (2014). Campanula rotundifolia L specie endangered with extinction, conserved through in vitro techniques. Analele Universității din Oradea, Fascicula: Protecția Mediului, 23:565-576.
• Mørk, E.K., Sriskandarajah, S., & Serek, M. (2005). Influence of seed germination conditions on regenerative ability in Campanula carpatica. European Journal of Horticultural Science, 70(4):173.
• Muller, S.A.,& Lipschutz, L. (1984). Potato. In: Ammirato, P.V., D.A. Evans, W.R. Sharp and Y. Yamada, (Eds.), Handbook of Plant Cell Culture, Collier Mcmillan Publishers, London, 3: 295.
• Özdöl, T., Altıoğlu, Y., Deniz, İ.G., & Yıldırım, H.A. (2022). New variety of Campanula myrtifolia (Campanulaceae) from South Anatolia, Turkiye. Herbarium Turcicum, 1:37-43.
• Paunescu, A. (2010). In vitro propagation of Campanula polymorpha Witas. An endemic plant of Carpathian Mountains. Analele Universităţii din Oradea - Fascicula Biologie, 17(2):235-238.
• Roest, S., & Bokelmann, G.S. (1976). Vegetative propagation of Solanum tuberosum L. in vitro. Potato Research, 19:173-178.
• Röper, A.C., Lütken, H., Christensen, B., Boutilier, K., Petersen, K.K., & Müller, R. (2015). Production of interspecific Campanula hybrids by ovule culture: exploring the effect of ovule isolation time. Euphytica, 203(3):643-657.
• Scariot, V., Seglie, L., Caser, M., & Devecchi, M. (2008). Evaluation of ethylene sensitivity and postharvest treatments to improve the vase life of four Campanula species. European Journal of Horticultural Science, 73(4):166.
• Seglie, L., Scariot, V., Larcher, F., Devecchi, M. and Chiavazza, P. M. (2012). In vitro seed germination and seedling propagation in Campanula spp. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 146(1):15-23.
• Sevindik, B., İzgü, T., Şimşek, Ö., Tütüncü, M., Çürük, P., Yılmaz, Ö., Kaynak, Y., Aka Kaçar, Y., Teixeira da Silva, J.A., Mendi, Y.Y. (2017). In vitro culture of Turkish Origanum sipyleum L. American Journal of Plant Biology, 2(5-1):32-36.
• Shim, J.N., Kim, G.H., & Jeong, B.R. (2005). Effect of CO2 concentration, NAEH and light intensity on the photoautotrophic growth of Campanula punctata'Rubriflora'plantlets in vitro. Journal of Bio-Environment Control, 14(4):233-238.
• Sivanesan, I., Lee, Y. M., Song, J.Y., & Jeong, B.R. (2007). Adventitious shoot regeneration from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino. Propagation Ornamental Plants, 7:210-215.
• Sivanesan, I., Lim, M.Y., & Jeong, B R. (2011). Somatic embryogenesis and plant regeneration from leaf and petiole explants of Campanula punctata Lam. var. rubriflora Makino. Plant Cell, Tissue, and Organ Culture, 107(2):365-369.
• Sriskandarajah, S., Frello, S., & Serek, M. (2001). Induction of adventitious shoots in vitro in Campanula carpatica. Plant Cell, Tissue and Organ Culture, 67(3):295-298.
• Stamenkovıć, V., Jelenčıć, B., & Tkalec, M. (2012). Propagation and antioxidative enzymes of Campanula velebitica Borbas grown in vitro. Periodicum Biologorum, 114(1):77-82.
• Tanaka, N., Matsuura, E., Terahara, N., & Ishimaru, K. (1999). Secondary metabolites in transformed root cultures of Campanula glomerata. Journal of plant Pphysiology, 155(2):251-254.
• Ullah, I., Jadoon, M., Rehman, A., Zeb, T., & Khan, K. (2012). Effect of different GA3 concentration on in vitro propagation of potato variety Desiree. Asian Journal of Agricultural Sciences, 4(2):108-109.
• Yıldırım, H. (2018). Campanula leblebicii (Campanulaceae), a new chasmophyte species from western Turkey. Phytotaxa, 376:114–122.
• Yıldırım, H., & Özdöl, T. (2019). A new suggestion IUCN threat category for Campanula peshmenii Güner (Campanulaceae). Acta Biologica Turcica, 32(3):168–173.
• Yıldırım, H., Şentürk, O., Özdöl, T., & Pirhan, A.F. (2019). A new bellflower, Campanula phitosiana sp. nov.(Campanulaceae) from Western Anatolia, Turkey. Phytotaxa, 399(1):25-36.