Harran Üniversitesi Tıp Fakültesi Dergisi 1304-9623 1309-4025 Harran University Clinical Sciences Klinik Tıp Bilimleri Investigation of DNA Damage in Rats in Chronically Exposed to Sub-Hypnotic Dose Desflurane Sıçanlarda, Subhipnotik Doz Kronik Desfluran Maruziyetinde, DNA Hasarının Araştırılması Altay Nuray Yıldırım Zeynep Baysal 04 15 2015 12 1 44 52 10 01 2014 11 05 2014 Copyright © 2004, Journal of Harran University Medical Faculty 2004 Journal of Harran University Medical Faculty

Background: In this study the aim was to research the DNA damage in rats whose chronic desfluran gasesexposed.Metods: This experimental study, after the Ethic Committe permission was made in Harran UniversityMedical Faculty Farmacology Department. For the study 200-230 gr 18 female albino wistar rats were used.After and before the study care and the feeding of the rats were made in the Harran University experimentalanimal laboratory. Rats divided in two groups (Group I: Desfluran, Grup II: Control ). For the anaesthesiapump system we used special planned plastic plate. Datex Ohmeda Tec 6 plus vaporizator was used to GroupI rats to whom hypnotic doses desflurane was given ( % 0,5-1 ). İn the second group same mechanism wasused but the desflurane vaporizator was closed. Each group %50 O2 and desflurane gas mixture was given inthe 5lt/minute speed. In the environment the desflurane and the O2 concentration were measured continouslyby the anaesthesia gases monitor ( criticare ). The condition of exposing to the gases, again 1 hour in 5 days.Outside of experiment, rats waited in room condition and in their cages. At the end of fifth day, 50mg/kg withpenthotal sodium, general anaesthesia was made and their extremities were fixed in the supine position fixingto the table. Blood samples were taken from intracardiac undertaking. The blood samples were takenheparinized tubes and put in cold condition in the special plate. Then sent to the Harran University MedicalFaculty Biochemical Department. The experimental rats sacrificed and then burried with lime. DNAdamagewas measured in taken bloods mononuclear leukocytes with Comet Assay method. İn addition, in same bloodsamples were measured antioxidant capacity and oxidative stress.Results: During the study, general anaesthesia conditions in any rat were not seen and rats were continuedtheir normal activity. DNAdamage, antioxidant capacity and oxidative stress were similar in both groups.Conclusions: It was concluded that not occur in a significant DNA damage, compared to the controlgroup in rats exposed chronically desflurane.

Amaç: Bu çalışmada, subhipnotik dozda, kronik desfluran gazına maruz kalan sıçanlardaki DNA hasarınınaraştırılması amaçlandı.Materyal ve metod: Deneysel çalışma, Harran Üniversitesi Tıp Fakültesi Etik Kurulu izni sonrasında aynıfakültenin Farmakoloji Laboratuarında yapıldı. Çalışma için 200-230gr ağırlığında 18 adet dişi Wistar türüalbino sıçan kullanıldı. Sıçanların çalışma öncesi ve sonrasındaki beslenme ve bakımları, HarranÜniversitesi Deney Hayvanları Laboratuarında yapıldı. Sıçanlar randomize 2 gruba ayrıldı (GrupI=Desfluran, Grup II= Kontrol). Anestezik gaz verme sistemi için, özel olarak tasarlanmış plastik kaplarkullanıldı. Grup I' de sıçanlara, Datex-Ohmeda Tec6 Plus vaporizatörü ile hipnoz oluşturmayacak dozlarda(%0.5-1) desfluran verildi. Grup II'de de aynı düzenek kullanıldı, ancak desfluran vaporizatörü kapalıtutuldu. Her iki grupta deney düzeneğine %50 oksijen-hava karışımı 5 litre /dakika taze gaz akışı hızındaverildi. Ortamdaki desfluran ve oksijen konsantrasyonları bir anestezik gaz monitörü (Criticare ®) ile sürekliolarak ölçüldü. Çalışma gazlarına maruz kalma durumu 5 gün boyunca günde 1 saat olacak şekildetekrarlandı. Deney dışındaki saatlerde sıçanlar kendi kafeslerinde oda şartlarında bakıldılar. Beşinci gününsonunda 50 mg/kg intraperitoneal pentothal ile anestezisi sağlanan sıçanlar, supin pozisyonundaekstremitelerden masaya tespit edildi. Sıçanlardan intrakardiak girişimle kan örnekleri alındı. Alınan kanlarheparinli tüplere aktarılarak özel bir kap içerisinde, soğuk ortamda, en kısa sürede Harran Üniversitesi TıpFakültesi Biyokimya Anabilim Dalı Laboratuarına ulaştırıldı. Deney hayvanları kan alındıktan sonrasakrifiye edilip, üzerlerine kireç kaymağı dökülüp toprağa gömüldüler. Alınan kanlardaki mononükleerlökositlerinden Comet Assey yöntemi ile DNAhasarları ölçüldü. Ayrıca aynı kan örneklerinden, antioksidankapasite ve oksidatif stress indeks değerleri analiz edildi.Bulgular: Deneysel çalışma sırasında hiçbir sıçanda genel anestezi durumu oluşmazken, tüm sıçanlar 5 günboyunca normal aktivitelerini sürdürdüler. Kan örneklerinden çalışılan DNAhasarı, antioksidan kapasite veoksidatif stres indeks değerleri, her iki grupta benzer bulundu.Sonuç: Kronik olarak desflurana maruz kalan sıçanlarda, kontrol grubuna göre, belirgin bir DNA hasarınınoluşmadığı sonucuna varıldı.

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