harran univ vet fak derg Harran University Journal of the Faculty of Veterinary Medicine 2146-7188 Harran University 10.31196/huvfd.508862 Zootechny (Other) Hayvansal Üretim (Diğer) Effects of Oleuropein on Hyperglycemia, Total Oxidant and Antioxidant Capacity in Streptozotosin-Diabetic Rats Treated with Metformin and Insulin Streptozotosin ile Diyabet Oluşturulmuş ve Metformin-İnsülin ile Diyabet Tedavisi Gören Ratlarda, Oleuropeinin Hiperglisemi, Total Oksidan ve Total Antioksidan Düzeyleri Üzerine Etkileri Kamiloğlu Nadide Nabil Department of Physiology, Faculty of Veterinary Medicine, Kafkas University, 36300, Kars, Turkey Dağdelen Hastürk Hülya Department of Physiology, Health Sciences Institute, Kafkas University, 36300, Kars, Turkey 12 27 2018 7 2 138 143 03 28 2018 11 25 2018 Copyright © 2012, Harran University Journal of the Faculty of Veterinary Medicine 2012 Harran University Journal of the Faculty of Veterinary Medicine

In thisstudy, it was aimed to investigate the effects of oleuropein on hyperglycemiaand oxidant-antioxidant levels in metformin-insulin treatedstreptozotocin-diabetic rats. For this purpose, 40 Sprague-Dawley rats wereused. Animals were divided into 5 groups as 4 experimental and 1 Control each containing8 rats. 1 ml isotonic NaCl solution was injected intraperitoneally (ip) to the Controlgroup while 50 mg/kg STZ ip was given to the G1, 50 mg/kg STZ + 100 mg/kgMetformin + 4 IU/kg insulin ip was given to the G2, 50 mg/kg STZ + 100 mg/kgMetformin + 4 IU/kg insulin ip and 30 mg/kg orally oleuropein was given to the G3and 50 mg/kg STZ ip and 30 mg/kg oleuropein orally was given to the G4. Bloodglucose values were statistically higher in the experimental groups than thecontrol after streptozotocin administration (P<0.001, P<0.01, P<0.01respectively). Plasma Total Antioxidant Capacity (TAC) of G2 and G3 were statistically higher thanG1 (P<0.001). It was found that plasma TAC levels of G3 were statisticallyhigher than the control (P<0.05). It was determined that the G1(P<0.001), G2 (P<0.01) and G3 (P<0.05) group had significantly higherplasma Total Oxidant Capacity (TOC) than the control group. TAC levels in theliver of the experimental groups were determined to be statisticallyinsignificant according to the control group. The liver TOC levels of the G2and G4 group were significantly higher than the control group (P<0.01, P<0.001respectively). In conclusion, the use of oleuropein alone did not affect theblood glucose level, the live weight, the plasma and the liver TAC and TOClevels, but the use of metformin and insulin with oleuropein as an antioxidantand free radical scavenger following to the rising oxidation in diabetes weresupported and strengthen the system.

Bu çalışmada,streptozotosin ile diyabet oluşturulmuş ve metformin ve insülin ile diyabettedavisi gören ratlarda, oleuropeinin hiperglisemi, oksidan-antioksidandüzeyleri üzerine etkilerinin araştırılması amaçlandı. Bu maksatla, 40 adeterkek Sprague Dawley rat kullanıldı. Ratlar her grupta 8 hayvan bulunacakşekilde 4 deney ve 1 kontrol grubu olmak üzere 5 gruba ayrıldı. Kontrol grubuna%0.09 NaCl intraperitoneal (ip) uygulandı. G1’e 50 mg/kg STZ ip, G2’ye 50 mg/kgSTZ + 100 mg/kg metformin + 4 İU/kg insülin ip, G3’e 50 mg/kg STZ + 100 mg/kgmetformin + 4 İU/kg insülin ip ve 30 mg/kg oleuropein oral olarak ve G4’e 50mg/kg STZ ip olarak ve 30 mg/kg oleuropein oral olarak uygulandı. Streptozotosinuygulandıktan sonraki 3, 10 ve 21. günlerde deneme gruplarının kan glukozdeğerleri kontrol grubuna göre istatistiksel olarak önemli düzeyde yüksekbulunmuştur (sırasıyla P<0.001, P<0.01, P<0.01). Plazma totalantioksidan kapasiteleri gruplar arasında kıyaslandığında, G2 ve G3 grubunun TotalAntioksidan Kapasite (TAC) düzeyleri, G1 grubuna göre istatistiksel olarak dahayüksek belirlenmiştir (P<0.001). Kontrol grubu ile deneme gurupları arasındakıyaslama yapıldığında G3 grubunun TAC düzeylerinin, kontrol grubuna göreistatistiksel olarak yüksek olduğu belirlenmiştir (P<0.05). G1 (P<0.001),G2 (P<0.01) ve G3 (P<0.05) grubunun plazma Total Oksidan Kapasitelerinin(TOC) kontrol grubuna göre istatistiksel olarak yüksek olduğu belirlenmiştir.Deneme gruplarının karaciğer TAC düzeylerinin kontrol grubuna göreistatistiksel olarak önemsiz olduğu tespit edildi. Karaciğer total oksidankapasiteleri gruplar arasında kıyaslandığında G2 grubu ile G4 gurubunun TOCdüzeylerinin kontrol grubuna göre istatistiksel olarak yüksek olduğu (sırasıylaP<0.01, P<0.001) belirlenmiştir. Sonuç olarak oleuropeinin tek başınakullanımının kan glukoz düzeyini, canlı ağırlığı ve plazma ile karaciğer TAC veTOC kapasitelerini etkilemediği, ancak diyabette yükselen oksidasyona karşılık,metformin ve insülinle birlikte kullanımının antioksidan sisteme destek olduğuve serbest radikal tutucu etkiyi kuvvetlendirdiği düşünülmektedir.

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