In this study,linseed oil was obtained with the aid of screw presses and linseed oilbiodiesel (B100) (linseed oil methyl ester) production was performed bytransesterification method. Linseed biodiesel was blended with regular dieselfuel at different ratios as B2 (98% diesel + 2% biodiesel), B5 (95% diesel + 5%biodiesel), B20 (80% diesel + 20% biodiesel), B50 (50% diesel + 50% biodiesel).Fuel properties tests were performed on all fuels. Results revealed that engineperformance values of linseed biodiesel and fuel blends were similar with thestandard diesel fuel. With regard to maximum torque, while the highest valuewas obtained as about 59.6 Nm at 1000 rpm with diesel fuel, the value wasobserved as about 53.8 Nm at 1200 1/min with B100 fuels. The highest maximumpower output was recorded as approximately 10.96 kW at 2100 rpm with dieselfuel and as approximately 10.23 kW at 2000 rpm with B100 fuels. With regard tominimum specific fuel consumption, while the lowest value was measured as about231.36 g/kWh at 1000 rpm with diesel fuel, the value was measured as about296.73 g/kWh at 1200 rpm with B100 fuels. Exhaust emissions are generallyimproved by the addition of linseed biodiesel to diesel fuel.