A comparative study on solvent extraction effect on the phytochemical profiling and antioxidant capacity of Zizyphus lotus L and Glycyrrhizaglabra L roots extracts from Algeria

Yıl 2020, Cilt: 4 Sayı: 2, 167 - 172, 30.12.2020

Mohammed Cheurfa , Rachida Allem Khawla Zabel , Waffa Aichouni , Abdalbasit Marıod

Öz

The aim of this research work is the determination of total phenols, flavonoids, condensed tannin levels, and antioxidant power of methanolic and aqueous extracts of Zizyphus lotus L and Glycyrrhiza glabra L roots, using three methods (Test DPPH, bleaching of β-carotene and hemoglobin denaturation protection). The higher levels of total phenols were found in methanol extracts of G. glabra and Z. lotus (58.94 ± 0.05 and 51.80 ± 0.02 mg GAE/ g of extract, respectively), the methanol extract of Z. lotus was the richest in flavonoids and tannins condensed with the content of 16.00 ± 0.01 and 17.22 ± 0.1 mg CE/g of extract respectively. With the DPPH method, the water extract of Z. lotus represents significantly (p <0.05) the most extract active (04.52 ± 0.17 mg/ml). For bleaching of β-carotene, G. glabra aqueous extract showed significantly (P <0.05) a higher antioxidant activity (71.47±01.86%). The methanol extracts of G. glabra and Z. lotus showed inhibition of hemoglobin denaturation with values of 71.80±0.06 and 71.71±0.33%, respectively. Based on these results, it could be concluded that the G. glabra and Z. lotus roots are a rich source of natural antioxidants of high value.

Anahtar Kelimeler

flavonoid,, phenolics,, Zizyphus lotus L,, Glycyrrhiz aglabra,, Free Radicals,, Antioxidant activity,

Kaynakça

• Akram, R., Saqib, M., Khalid, B., & Arshad, A. (2015). Effects of Extraction Media and Techniques on the Antioxidant Properties and Recovery of Phenolics from Roots of Glycyrrhiza Glabra. Journal of Molecular Pathophysiology, 4(4), 138. https://doi.org/10.5455/jmp.20151215121630
• Atmani, D., Chaher, N., Berboucha, M., Ayouni, K., Lounis, H., Boudaoud, H., … Atmani, D. (2009). Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chemistry, 112(2), 303–309. https://doi.org/10.1016/J.FOODCHEM.2008.05.077
• Bakhtaoui, F. Z., Lakmichi, H., Megraud, F., Chait, A., & Gadhi, C. E. A. (2014). Gastro-protective, anti-Helicobacter pylori and, antioxidant properties of Moroccan Zizyphus lotus L. Journal of Applied Pharmaceutical Science, 4(10), 81–87. https://doi.org/10.7324/JAPS.2014.40115
• Bors, W., Foo, Y., Hertkorn, N., Michel, C., & Stett-maier, K. (2001). Chemical studies of proanthocyanidins and hydrolysable tannins. Antioxid. Redox. Sign, 3, 995–1008. Boyd, B., Ford, C., Koepke, M., Gary, K., Horn, E., & et al. (2003). Etude pilote ouverte de l’effet antioxydant d’Ambrotose sur des personnes en bonne santé. Glycoscience & Nutrition, 4(6), 7.
• Cheurfa, M., & Allem, R. (2016). Evaluation of antioxidant activity of different extracts of Aloysia triphylla leaves (L’Herit.) from Algeria in vitro. Phytotherapie, 14(3). https://doi.org/10.1007/s10298-015-0969-4
• De Quirós, A. R.-B., Lage-Yusty, M. A., & López-Hernández, J. (2009). HPLC-analysis of polyphenolic compounds in Spanish white wines and determination of their antioxidant activity by radical scavenging assay. Food Research International, 42(8), 1018–1022. https://doi.org/10.1016/j.foodres.2009.04.009
• El-Waziry, A. . (2007). Nutritive value assessment of ensiling or mixing Acacia and Atriplex using in vitro gas production technique Title. Res. J. Agric. Biol. Sci, 3(6), 605–614.
• Fant, J., Grandon, B., Rathinasabapathi, B., Olson, S., & Lyrene, P. (2005). Potentiel antioxydant des fruits: l’évaluation de deux cultivars de bleuets à l’aide de test in vitro. Proc. Fla Etat Hort. Soc, 118, 250–252.
• Favier, A. (2003). Le stress oxydant Intérêt conceptuel et expérimental dans la compréhension. Journal de La Société Chimique de France, (Novembre/Decembre), 108–115. https://doi.org/10.1016/j.cub.2014.07.028
• Ferreria, A., Proenca, C., Serralheiro, L., & Aranjo, M. (2008). The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plant from Portugal. Journal of Ethnopharmacology, 108, 31–37.
• Gormez, A. A., & Sengul, M. (2007). Total phenolics, mineral contents, antioxidant and antibacterial activities of Glycyrrhiza glabra L . roots grown wild in Turkey Volume. (December 2014).
• Heim, K. E., Tagliaferro, A. R., & Bobilya, D. J. (2002). Flavonoid antioxidants: chemistry, metabolism, and structure-activity relationships. The Journal of Nutritional Biochemistry, 13(10), 572–584. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12550068
• Kang, D. G., Yun, C. keun, & Lee, H. S. (2003). Screening and comparison of antioxidant activity of solvent extracts of herbal medicines used in Korea. Journal of Ethnopharmacology, 87(2–3), 231–236. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12860314
• Ksouri, R., Megdiche, W., Falleh, H., Trabelsi, N., Boulaaba, M., Smaoui, A., & Abdelly, C. (2008). Influence of biological, environmental, and technical factors on phenolic content and antioxidant activities of Tunisian halophytes. Comptes Rendus Biologies, 331(11), 865–873. https://doi.org/10.1016/j.crvi.2008.07.024
• Naz, S., Sultana, B., Shahid, M., & Ur-Rehman, K. (2013). Alteration in antioxidant and antimicrobial attributes of leaves of Zizyphus species in response to maturation. Journal of Medicinal Plants Research, 7(2), 61–70.
• Sanchez-Moreno, C. (2002). Review: Methods Used to Evaluate the Free Radical Scavenging Activity in Foods and Biological Systems. Food Science and Technology International, 8(3), 121–137. https://doi.org/10.1106/108201302026770
• Sroka, Z. (2005). Antioxidative and antiradical properties of plant phenolics. Zeitschrift Fur Naturforschung. C, Journal of Biosciences, 60(11–12), 833–843. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/16402542
• Suhayla, K. M. (2014). Antioxidative Activities of Aqueous and Ethanolic Extracts of Licorice Roots. Pakistan Journal of Nutrition, 13(5), 267–270. https://doi.org/10.3923/pjn.2014.267.270
• Sultana, S., Haque, A., Hamid, K., Urmi, K., & Roy, S. (2010). Antimicrobial, cytotoxic, and antioxidant activity of methanolic extract of Glycyrrhiza glabra. Agriculture and Biology Journal of North America, 1(5), 957–960. https://doi.org/10.5251/abjna.2010.1.5.957.960
• Sun, B., Ricardo-da-Silva, J., & Spranger, I. (1998). Critical Factors of Vanillin Assay for Catechins and Proanthocyanidins. J. Agric. Food Chem, 46(10), 4267–4274. https://doi.org/10.1021/JF980366J
• Tepe, B., Daferera, D., Sökmen, M., Polissiou, M., & Sökmen, A. (2004). In Vitro Antimicrobial and Antioxidant Activities of the Essential Oils and Various Extracts of Thymus eigii M. Zohary et P.H. Davis. Journal of Agricultural and Food Chemistry, 52(5), 1132–1137. https://doi.org/10.1021/jf035094l
• Walker, J. E., Saraste, M., Runswick, M. J., & Gay, N. J. (1982). Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. The EMBO Journal, 1(8), 945–951. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/6329717
• Wong, S., Leong, L., & Williamkoh, J. (2006). Antioxidant activities of aqueous extracts of selected plants. Food Chemistry, 99(4), 775–783. https://doi.org/10.1016/j.foodchem.2005.07.058
• Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555–559. https://doi.org/10.1016/S0308-8146(98)00102-2
• Zhou, X., Peng, J., Fan, G., & Wu, Y. (2005). Isolation and purification of flavonoid glycosides from Trollius ledebouri using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phase. Journal of Chromatography A, 1092(2), 216–221. https://doi.org/10.1016/j.chroma.2005.07.064