A comparative study on solvent extraction effect on the phytochemical profiling and antioxidant capacity of Zizyphus lotus L and Glycyrrhizaglabra L roots extracts from Algeria

Year 2020, Volume: 4 Issue: 2, 167 - 172, 30.12.2020

Mohammed Cheurfa , Rachida Allem Khawla Zabel , Waffa Aichouni , Abdalbasit Marıod

Abstract

The aim of this research work is the determination of total phenols, flavonoids, condensed tannin levels, and antioxidant power of methanolic and aqueous extracts of Zizyphus lotus L and Glycyrrhiza glabra L roots, using three methods (Test DPPH, bleaching of β-carotene and hemoglobin denaturation protection). The higher levels of total phenols were found in methanol extracts of G. glabra and Z. lotus (58.94 ± 0.05 and 51.80 ± 0.02 mg GAE/ g of extract, respectively), the methanol extract of Z. lotus was the richest in flavonoids and tannins condensed with the content of 16.00 ± 0.01 and 17.22 ± 0.1 mg CE/g of extract respectively. With the DPPH method, the water extract of Z. lotus represents significantly (p <0.05) the most extract active (04.52 ± 0.17 mg/ml). For bleaching of β-carotene, G. glabra aqueous extract showed significantly (P <0.05) a higher antioxidant activity (71.47±01.86%). The methanol extracts of G. glabra and Z. lotus showed inhibition of hemoglobin denaturation with values of 71.80±0.06 and 71.71±0.33%, respectively. Based on these results, it could be concluded that the G. glabra and Z. lotus roots are a rich source of natural antioxidants of high value.

Keywords

flavonoid,, phenolics,, Zizyphus lotus L,, Glycyrrhiz aglabra,, Free Radicals,, Antioxidant activity,

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