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Determination of the Genetic Variation within the Egg Parasitoid, Trichogramma brassicae, Bezdenko (Hymenoptera: Trichogrammatidae) Populations in Düzce Province, Turkey

Year 2018, Volume: 4 Issue: 1, 26 - 32, 20.06.2018
https://doi.org/10.24180/ijaws.395438

Abstract

Members of Trichogramma genus are the most widely used as natural enemies in biological control programs. Choice of natural enemies is known to have a critical important for the succession of biological control. Their morphologic identification has some bottlenecks as laborious, time-consuming and mostly based on very small structures of the bodies. Molecular techniques have been widely used for detection, discrimination, phylogenetic analysis, identification and characterization in biological control agents belong to Trichogramma spp. In this study, the efficacy of the direct sequencing analysis of internal transcribed spacer locus of rDNA was investigated for analyzing the genetic variation within nine Trichogramma brassicae population from corn growing areas in Düzce province. The comparison of the sequences was performed with other ITS2 locus of Trichogramma species available in GenBank database. The result of analyses indicated that no genetic variation was determined in the Düzce population.

References

  • Alvarez JM and Hoy MA., 2002. Evaluation of the ribosomal ITS2 DNA sequences in closely related populations of the parasitoid Ageniaspis (Hymenoptera: Encyrtidae). Annals of the Entomological Society of America, 95(2): 250-256.
  • Chang SC., Hu NT, Hsin CY and Sun CN., 2001. Characterization of differences between two Trichogramma wasps by molecular markers. Biol. Control. 21: 75-78.
  • Cruickshank RH., 2002. Molecular markers for the phylogenetics of mites and ticks. Systematic & Applied Acarology, 7: 3-14.
  • Hall TA., 1999. BioEdit: A User-friendly Biological Sequence Alignment [ed.], and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95-98.
  • Jeong G., Kim H, Choi Y, Kim W, Park K, Bae S, Park K and Choi J., 2010. Molecular identification of two Trichogramma species (Hymenoptera: Trichogrammatidae) in Korea. Journal of Asia-Pacific Entomology, 13(1): 41-44.
  • Knutson A., 1998. The Trichogramma manual, Agricultural communication, The Texas A&M University System, Texas.
  • Kimura M., 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 111-120.
  • Kumar GA., Jalali SK, Venkatesan T, Stouthamer R, Niranjana P and Lalitha Y., 2009. Internal transcribed spacer-2 restriction fragment length polymorphism (ITS-2-RFLP) tool to differentiate some exotic and indigenous trichogrammatid egg parasitoids in India. Biological control, 49(3): 207-213.
  • Kumar S., Stecher G and Tamura K., 2016 MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33: 1870-1874.
  • Kutuk H., 2016. Identification and natural parasitism of Trichogramma species on Ostrinia nubilalis Hübner (Lepidoptera: Noctuidae) eggs in Düzce, Turkey. Entomological News, 126(4): 290-298.
  • Li Y., 1994. Worldwide use of Trichogramma for Biological Control on Different Crops: A survey. In: Wajnberg, E. and Hassan, S.A. (Eds.). Biological Control with Eggs Parasitoids. Center for Agriculture and Biosciences International. Wallingford, Great Britain. pp. 37-54.
  • Meksem K and Kahl G., 2005. The Handbook of Plant Genome Mapping, Genetic and Physical Mapping, Wiley-VCH, Weinheim, 380 p. Nagarkatti S and Nagaraja h., 1977. Biosystematics of Trichogramma and Trichogrammatoidea species. Annual Review of Entomology, 22: 157-176.
  • Pinto JD and Stouthamer R., 1994. Systematics of the Trichogrammatidae with Emphasis on Trichogramma. In: Wajnberg E, Hassan SA (eds) Trichogramma and other Egg Parasitoids. CAB Intl., London, pp. 1-36.
  • Pinto JD., 1999. Systematics of the North American species of Trichogramma Westwood (Hymenoptera: Trichogrammmatidae). Memoirs of the Entomological Society of Washington, 22: 287-301.
  • Smith SM., 1996. Biological control with Trichogramma: advances, successes, and potential of their use. Annual Review of Entomology, 41: 375-406.
  • Smith SM and Hubbes M., 1986. Isoenzyme patterns and biology of Trichogramma minutum as influenced by rearing temperature and host. Entomologia Experimentalis et Applicata, 42: 249-258.
  • Stouthamer R., Hu J, van Kan FJPM, Platner GR and Pinto JD., 1999. The utility of Internally Transcribed Spacer 2 DNA sequences of the nuclear ribosomal gene for distinguishing sibling species of Trichogramma (Hymenoptera: Trichogrammatidae). BioControl, 43: 421-440.
  • Subbotin SA., Sturhan D, Rumpenhorst HJ and Moens M., 2003. Molecular and morphological characterisation of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Nematology, 5: 515-538.
  • Sumer F., Tuncbilek AS, Oztemiz S, Pintureau B, Rugman-Jones P and Stouthamer R., 2009. A molecular key to the common species of Trichogramma of the Mediterranean region. BioControl, 54: 617-624.
  • Sumer Ercan F., Oztemiz S, Tuncbilek AS and Stouthamer R., 2011. Sequence analysis of the ribosomal DNA ITS2 region in two Trichogramma species (Hymenoptera: Trichogrammatidae). Archieves of Biological Sciences, 63(4), 949-954.
  • Sumer Ercan F., Oztemiz S and Tuncbilek A.S., 2013. Mitochondrial and ribosomal sequence analysis for discrimination of Trichogramma euproctis Girault and Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae). Turkish Journal of Entomology, 37: 195-201.
  • Tanha Maafi Z., Subbotin SA and Moens M., 2003. Molecular identification of cyst forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology, 5: 99-111.
  • Thomson L., Rundle BJ, Carew ME and Hoffmann AA., 2003. Identification and characterization of Trichogramma species from south-eastern using the internal transcribed spacer 2 (ITS2) region of the ribosomal gene complex. Entomologia Experimentalis et Applicata, 106(3): 235-240.
  • Waeyenberge L., Ryss A, Moens M, Pinochet J and Vrain TC., 2000. Molecular characterization of 18 Pratylenchus species using rDNA restriction fragment length polymorphism. Nematology, 2: 135-2.

Yumurta Parazitoiti, Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae)’nin Düzce İlindeki Popülasyonları İçerisindeki Genetik Varyasyonların Belirlenmesi

Year 2018, Volume: 4 Issue: 1, 26 - 32, 20.06.2018
https://doi.org/10.24180/ijaws.395438

Abstract

Trichogramma türleri, biyolojik mücadele programlarında en çok kullanılan doğal düşmanlardandır. Doğal düşmanın seçiminin, biyolojik kontrolün başarısı açısından kritik bir öneme sahip olduğu bilinmektedir. Bu cinse bağlı türlerin morfolojik teşhisleri zahmetli olmaları, zaman kaybı oluşturmaları ve vücut yapılarının oldukça küçük boyutlarda olmasına bağlı olarak problem oluşturmaktadır. Moleküler teknikler, Trichogramma cinsine ait biyolojik kontrol ajanlarında tespit etme, ayırt etme, filogenetik analiz, tanımlama ve karakterizasyon için yaygın olarak kullanılmaktadır. Bu çalışmada, Düzce ilinde mısır yetiştirilen alanlardan elde edilen dokuz Trichogramma brassicae popülasyonunun genetik varyasyon analizi için rDNA’nın ITS bölgesinin
doğrudan sekanslama analizinin etkinliği araştırılmıştır. Sekansların karşılaştırılması, GenBank veri tabanında mevcut olan Trichogramma türlerinin ITS2 bölgeleri ile gerçekleştirilmiştir. Analiz sonuçları, Düzce popülasyonlarında genetik varyasyonun tespit edilmediğini göstermiştir.

References

  • Alvarez JM and Hoy MA., 2002. Evaluation of the ribosomal ITS2 DNA sequences in closely related populations of the parasitoid Ageniaspis (Hymenoptera: Encyrtidae). Annals of the Entomological Society of America, 95(2): 250-256.
  • Chang SC., Hu NT, Hsin CY and Sun CN., 2001. Characterization of differences between two Trichogramma wasps by molecular markers. Biol. Control. 21: 75-78.
  • Cruickshank RH., 2002. Molecular markers for the phylogenetics of mites and ticks. Systematic & Applied Acarology, 7: 3-14.
  • Hall TA., 1999. BioEdit: A User-friendly Biological Sequence Alignment [ed.], and Analysis Program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41: 95-98.
  • Jeong G., Kim H, Choi Y, Kim W, Park K, Bae S, Park K and Choi J., 2010. Molecular identification of two Trichogramma species (Hymenoptera: Trichogrammatidae) in Korea. Journal of Asia-Pacific Entomology, 13(1): 41-44.
  • Knutson A., 1998. The Trichogramma manual, Agricultural communication, The Texas A&M University System, Texas.
  • Kimura M., 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 111-120.
  • Kumar GA., Jalali SK, Venkatesan T, Stouthamer R, Niranjana P and Lalitha Y., 2009. Internal transcribed spacer-2 restriction fragment length polymorphism (ITS-2-RFLP) tool to differentiate some exotic and indigenous trichogrammatid egg parasitoids in India. Biological control, 49(3): 207-213.
  • Kumar S., Stecher G and Tamura K., 2016 MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33: 1870-1874.
  • Kutuk H., 2016. Identification and natural parasitism of Trichogramma species on Ostrinia nubilalis Hübner (Lepidoptera: Noctuidae) eggs in Düzce, Turkey. Entomological News, 126(4): 290-298.
  • Li Y., 1994. Worldwide use of Trichogramma for Biological Control on Different Crops: A survey. In: Wajnberg, E. and Hassan, S.A. (Eds.). Biological Control with Eggs Parasitoids. Center for Agriculture and Biosciences International. Wallingford, Great Britain. pp. 37-54.
  • Meksem K and Kahl G., 2005. The Handbook of Plant Genome Mapping, Genetic and Physical Mapping, Wiley-VCH, Weinheim, 380 p. Nagarkatti S and Nagaraja h., 1977. Biosystematics of Trichogramma and Trichogrammatoidea species. Annual Review of Entomology, 22: 157-176.
  • Pinto JD and Stouthamer R., 1994. Systematics of the Trichogrammatidae with Emphasis on Trichogramma. In: Wajnberg E, Hassan SA (eds) Trichogramma and other Egg Parasitoids. CAB Intl., London, pp. 1-36.
  • Pinto JD., 1999. Systematics of the North American species of Trichogramma Westwood (Hymenoptera: Trichogrammmatidae). Memoirs of the Entomological Society of Washington, 22: 287-301.
  • Smith SM., 1996. Biological control with Trichogramma: advances, successes, and potential of their use. Annual Review of Entomology, 41: 375-406.
  • Smith SM and Hubbes M., 1986. Isoenzyme patterns and biology of Trichogramma minutum as influenced by rearing temperature and host. Entomologia Experimentalis et Applicata, 42: 249-258.
  • Stouthamer R., Hu J, van Kan FJPM, Platner GR and Pinto JD., 1999. The utility of Internally Transcribed Spacer 2 DNA sequences of the nuclear ribosomal gene for distinguishing sibling species of Trichogramma (Hymenoptera: Trichogrammatidae). BioControl, 43: 421-440.
  • Subbotin SA., Sturhan D, Rumpenhorst HJ and Moens M., 2003. Molecular and morphological characterisation of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Nematology, 5: 515-538.
  • Sumer F., Tuncbilek AS, Oztemiz S, Pintureau B, Rugman-Jones P and Stouthamer R., 2009. A molecular key to the common species of Trichogramma of the Mediterranean region. BioControl, 54: 617-624.
  • Sumer Ercan F., Oztemiz S, Tuncbilek AS and Stouthamer R., 2011. Sequence analysis of the ribosomal DNA ITS2 region in two Trichogramma species (Hymenoptera: Trichogrammatidae). Archieves of Biological Sciences, 63(4), 949-954.
  • Sumer Ercan F., Oztemiz S and Tuncbilek A.S., 2013. Mitochondrial and ribosomal sequence analysis for discrimination of Trichogramma euproctis Girault and Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae). Turkish Journal of Entomology, 37: 195-201.
  • Tanha Maafi Z., Subbotin SA and Moens M., 2003. Molecular identification of cyst forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology, 5: 99-111.
  • Thomson L., Rundle BJ, Carew ME and Hoffmann AA., 2003. Identification and characterization of Trichogramma species from south-eastern using the internal transcribed spacer 2 (ITS2) region of the ribosomal gene complex. Entomologia Experimentalis et Applicata, 106(3): 235-240.
  • Waeyenberge L., Ryss A, Moens M, Pinochet J and Vrain TC., 2000. Molecular characterization of 18 Pratylenchus species using rDNA restriction fragment length polymorphism. Nematology, 2: 135-2.
There are 24 citations in total.

Details

Primary Language English
Journal Section Plant Protection
Authors

Abdurrahman Sami Koca 0000-0002-7657-5615

Mustafa İmren 0000-0002-7217-9092

Halil Kütük 0000-0002-2390-2053

Publication Date June 20, 2018
Submission Date February 15, 2018
Acceptance Date April 17, 2018
Published in Issue Year 2018 Volume: 4 Issue: 1

Cite

APA Koca, A. S., İmren, M., & Kütük, H. (2018). Determination of the Genetic Variation within the Egg Parasitoid, Trichogramma brassicae, Bezdenko (Hymenoptera: Trichogrammatidae) Populations in Düzce Province, Turkey. Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi, 4(1), 26-32. https://doi.org/10.24180/ijaws.395438

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