Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers

Year 2019, Volume: 5 Issue: 1, 90 - 95, 30.06.2019

Mustafa İmren , Abdurrahman Sami Koca , Abdelfattah Amer Dababat

https://doi.org/10.24180/ijaws.512967

Cited By: 2

Abstract

The
Mediterranaen cereal cyst nematode, Heterodera
latipons, causing serious yield losses in protected cultivation cereal
cropping areas in Turkey. An accurate and reliable identification is primarily
needed to establish effective, sustainable and environmentally safe control
measures to any cyst nematode species. In this study, twenty-five populations of
Heterodera latipons collected
from cereal areas in East-Mediterranean and South-East Anatolian regions were
identified using species-specific primers (SCAR). The
SCAR primer pair, H-latac F and H-latact R, defined species-specific primers
were used to identify Heterodera
populations. In this study stated that H-latac F and H-latact R primers for
identifying of H. latipons can be
efficient tools to identify the Turkish Mediterranean cereal cyst nematode
populations. Moreover, the results indicated that H. latipons was the prominent cereal cyst nematode species in the
East Mediterranean and South East Anatolian regions of Turkey. This
clarified approach may supply a quick basis for identification of H. latipons populations for their future
management and facilitate checking its distribution in the agricultural areas.

Keywords

Heterodera latipons, identification, SCAR primer, SCAR primer

Heterodera latipons’un SCAR (Sequence Characterised Amplified Region) Primer Kullanılarak Tanımlanması

Abstract

Akdeniz Tahıl kist nematodu Heterodera
latipons, mücadelesinin yapılmadığı buğday üretim alanlarında önemli derece
ürün kayıplarına neden olabilebilmektedir. Nematodlar ile mücadelede
sürüdürülebilir ve etkili bir mücadele için nematodun türünün bilinmesi oldukça
önemlidir. Bu çalışmada, Doğu Akdeniz ve Güney Doğu Anadolu bölgeleri tahıl
alanlarından elde edilen 25 adet Heterodera latipons popülasyonu, türe
spesifik primer çifti (SCAR) H-latacF ve H-latactR kullanılarak tanımlanmıştır.
Çalışma sonucunda, Heterodera latipons popülasyonlarının tanımlanmasında
H-latacF ve H-latactR primerleri etmeni başarılı bir şekilde tanımlamış olup,
nematodun Doğu Akdeniz ve Güney Doğu Anadolu bölgelerindeki en önemli Tahıl
kisti nematod türü olduğu ortaya konulmuştur. Ayrıca, çalışmada kullanılan türe
özgü primerler Heterodera latipons popülasyonlarının kolay ve hızlı
tanımlanmasında kullanılabileceği sonucuna varılmıştır. 

Keywords

Heterodera latipons, teşhis, SCAR primer

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