Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers

Year 2019, Volume: 5 Issue: 1, 90 - 95, 30.06.2019

Mustafa İmren Abdurrahman Sami Koca Abdelfattah Amer Dababat

https://doi.org/10.24180/ijaws.512967

Cited By: 2

Abstract

The
Mediterranaen cereal cyst nematode, Heterodera
latipons, causing serious yield losses in protected cultivation cereal
cropping areas in Turkey. An accurate and reliable identification is primarily
needed to establish effective, sustainable and environmentally safe control
measures to any cyst nematode species. In this study, twenty-five populations of
Heterodera latipons collected
from cereal areas in East-Mediterranean and South-East Anatolian regions were
identified using species-specific primers (SCAR). The
SCAR primer pair, H-latac F and H-latact R, defined species-specific primers
were used to identify Heterodera
populations. In this study stated that H-latac F and H-latact R primers for
identifying of H. latipons can be
efficient tools to identify the Turkish Mediterranean cereal cyst nematode
populations. Moreover, the results indicated that H. latipons was the prominent cereal cyst nematode species in the
East Mediterranean and South East Anatolian regions of Turkey. This
clarified approach may supply a quick basis for identification of H. latipons populations for their future
management and facilitate checking its distribution in the agricultural areas.

Keywords

Heterodera latipons, identification, SCAR primer, SCAR primer

Heterodera latipons’un SCAR (Sequence Characterised Amplified Region) Primer Kullanılarak Tanımlanması

Abstract

Akdeniz Tahıl kist nematodu Heterodera
latipons, mücadelesinin yapılmadığı buğday üretim alanlarında önemli derece
ürün kayıplarına neden olabilebilmektedir. Nematodlar ile mücadelede
sürüdürülebilir ve etkili bir mücadele için nematodun türünün bilinmesi oldukça
önemlidir. Bu çalışmada, Doğu Akdeniz ve Güney Doğu Anadolu bölgeleri tahıl
alanlarından elde edilen 25 adet Heterodera latipons popülasyonu, türe
spesifik primer çifti (SCAR) H-latacF ve H-latactR kullanılarak tanımlanmıştır.
Çalışma sonucunda, Heterodera latipons popülasyonlarının tanımlanmasında
H-latacF ve H-latactR primerleri etmeni başarılı bir şekilde tanımlamış olup,
nematodun Doğu Akdeniz ve Güney Doğu Anadolu bölgelerindeki en önemli Tahıl
kisti nematod türü olduğu ortaya konulmuştur. Ayrıca, çalışmada kullanılan türe
özgü primerler Heterodera latipons popülasyonlarının kolay ve hızlı
tanımlanmasında kullanılabileceği sonucuna varılmıştır. 

Keywords

Heterodera latipons, teşhis, SCAR primer

References

• Al-Banna, L., Ploeg, A. T., Williamson, V. M., & Kaloshian, I. (2004). Discrimination of six pratylenchus species using PCR and species-specific primers. Journal of Nematology, 36(2), 142-146.
• Blok, V. C., & Powers, T. O. (2009). Biochemical and molecular identification. In R. N. Perry, M. Moens & J. Starr (Eds.), Root-Knot Nematodes (pp 98-112). Lincoln: CABI.
• Cherry, T., Szalanski, A. L., Todd, T. C., & Powers, T. O. (1997). The internal transcribed spacer region of Belonolaimus (Nemata: Belonolaimidae). Journal of Nematology, 29, 21-29.
• Cook, R., & Noel, G. R. (2002). Cyst Nematodes: Globodera and Heterodera species. In J. L. Star, R. Cook & J. Bridge (Eds.), Plant Resistance to Parasitic Nematodes (pp. 71-105). CAB International.
• Cohn, E., & Ausher, R. (1973). Longidorus cohni and Heterodera latipons economic nematode pest of oat Israil. Plant Disease Report, 57, 53-54.
• Dababat, A. A., Orakcı, G. E., Toktay, H., Imren, M., Akın, B., Braun, HJ., Dreisigacker, S., Elekcioglu, I. H., & Morgunov, A. (2014). Resistance of winter wheat to Heterodera filipjevi in Turkey. Turkish Journal of Agriculture and Forestry, 38, 180-186.
• Dababat, A. A., Imren, M., Orakci, E. G., Ashrafi, S., Yavuzaslanoglu, E., Toktay, H., Pariyar, S., Elekcioğlu, I. H., Morgounov, A., & Mekete T. (2015). The importance and management strategies of cereal cyst nematodes, Heterodera spp., in Turkey. Euphytica, 202, 173-188.
• Fenwick, D. W. (1940). Methods for the recovery and counting of cysts of H. schachtii from soil. Journal of Helminthology, 18, 155-172.
• Franklin, M. T. (1969). Heterodera latipons n. sp., a cereal cyst nematode from the Mediterranean region. Nematologica, 15, 535-542.
• Hando, Z. A. (2002). A key compendium to species of the Heterodera avenae group (Nematode: Heteroderidae). Journal of Nematology, 34, 250-262.
• Imren, M., Toktay, H., Özarslandan, A., Nicol, J. M., & Elekcioglu, İ. H. (2012). Güneydoğu Anadolu Bölgesi tahıl alanlarında tahıl kist nematodu, Heterodera avenae group türlerinin belirlenmesi. Türkiye Entomoloji Dergisi, 36(2), 265-275.
• Imren, M., & Elekcioğlu, İ. H. (2015). Effect of cereal cyst nematode Heterodera avenae (Tylenchida: Heteroderidae) on yield of some spring wheat varieties in Adana Province, Turkey. Turkish Journal of Agriculture and Forestry, 38(6), 820-823.
• Imren, M., Waeyenberge, L., Viaene, N., Elekcioğlu, İ. H., & Dababat, A. A. (2015). Morphological and molecular identification of cereal cyst nematodes from the eastern Mediterranean region of Turkey. Turkish Journal of Agriculture and Forestry, 39, 91-98.
• Kovaleva, E. S., Subbotin, A. S., Edward, P. M., & Chitwood, D. (2005). Molecular characterization of the actin gene from cyst nematodes in comparison with those from other nematodes. Comparative Parasitology, 72(1), 39-50.
• Maafi, Z. T., Subbotin, S. A., & Moens, M. (2003). Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology, 5(1), 99-111.
• McDonald, A. H., & Nicol, J. M. (2005). Nematode parasites of cereals. In M. Luc, R. A. Sikora & J. Bridge (Eds.). Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (pp. 131-191) CAB International.
• Mundo-Ocampo, M., Troccoli, A., Subbotin, S. A., Del Cid, J., Baldwin, J. G., & Inserra, R. N. (2008). Synonymy of Afenestrata with Heterodera supported by phylogenetics with molecular and morphological characterisation of H. koreana comb. n. and H. orientalis comb. n. (Tylenchida: Heteroderidae). Nematology, 10(5), 611-632.
• Mor, M., Cohn, E., & Spiegel, Y. (1992). Phenology, pathogenicity and pathotypes of cereal cyst nematodes, Heterodera avenae and H. latipons (Nematoda: Heteroderidae) in Israel. Nematologica, 38, 494-501.
• Nicol, J. M., Rivoal, R., Bolat, N., Aktas, H., Braun, H., Mergoum, J. M., Yildirim, A. F., Bagci, A., Elekcioglu, İ. H., & Yahyaoui, A. (2002). The frequency and diversity of the cyst and lesion nematodes on wheat in the Turkish central Anatolian plateau. Nematology, 4, 272.
• Philis, J. (1995). An updated list of plant parasitic nematodes from Cyprus and their economic importance. Nematologia Mediterranea, 23, 307-314.
• Powers, T. O., Todd, T. C., Burnell, A. M., Murray, P. C. B., Fleming, C. C., Szalanski, A. L., Adams, B. A., & Harris, T. S. (1997). The internal transcribed spacer region as a taxonomic marker for nematodes. Journal of Nematology, 29, 441-450.
• Rivoal, R., & Cook, R. (1993). Nematode pests of cereals. In K. Evans, D. L. Trudgill & J. M. Webster (Eds.), Plant Parasitic Nematodes in Temperate Agriculture (pp. 259-303). CAB International: Wallingford, United Kingdom.
• Rivoal, R., Valette, S., Bekal, S., Gauthier, J. P. & Yahyaoui, A. (2003). Genetic and phenotypic diversity in the graminaceous cyst nematode complex, inferred from PCR-RFLP of ribosomal DNA and morphometric analysis. European Journal of Plant Pathology, 109, 227-241.
• Rumpenhorst, H. J., Elekçioğlu, I. H., Sturhan, D., Öztürk, G., & Enneli, S. (1996). The cereal cyst nematode Heterodera filipjevi (Madzhidov) in Turkey. Nematologia Mediterránea, 24, 135-138.
• Smiley, R. W., Gourlie, J. A., Whittaker, R. G., Easley, S. A., & Kidwell, K. K. (2004). Economic impact of Hessian fly (Diptera: Cecidomyiidae) on spring wheat in Oregon and additive yield losses with Fusarium crown rot and lesion nematode. Journal of Economic Entomology, 97(2), 397-408.
• Subbotin, S. A., Mundo-Ocampo, M., & Baldwin, J. G. (2010). Systematics of cyst nematodes (Nematode: Heteroderinae) nematology monographs and perspectives 8A. In: Biology and Evolution. Brill Leiden–Boston.
• Szalanski, A. L., Sui, D. D., Harris, T. S., & Powers, T. O. (1997). Identification of cyst nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1. Journal of Nematology, 29, 255-267.
• Toumi, F., Waeyenberge, L., Viaene, N., Dababat, A. A., Nicol, J. M., Ogbonnaya, F., & Moens, M. (2013). Development of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevi. European Journal of Plant Pathology, 136(3), 613-624.
• Toumi, F., Waeyenberge, L., Viaene, N., Dababat, A. A., Nicol, J. M., Ogbonnaya, F. C., & Moens, M. (2015). Development of qPCR assays for quantitative detection of Heterodera avenae and H. latipons. European Journal of Plant Pathology, 143(2), 305-316.
• Tytgat, T., Vanholme, B., & De Meutter, J. (2004). new class of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in plant parasitic cyst nematodes. Molecular Plant-Microbe Interactions,17, 846-852.
• Yousef, D. M., & Jacob, J. J. S. (1994). A nematode survey of vegetable crops and some orchards in the ghour of Jordan. Journal of Nematologia Mediterranea, 22, 11-15.
• Waeyenberge, L., Viaene, N., Subbotin, S. A., & Moens, M. (2009). Molecular Identification of Heterodera spp., an Overview of Fifteen Years of Research. Cereal Cyst Nematodes: Status, Research and Outlook. In Proceedings of the First Workshop of The International Cereal Cyst Nematode Initiative, 21, 109-11.