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Doğal Florada Çimlenmiş Sekiz Farklı Salep Orkidesi Türünün İlk Yıl Gelişimlerinin Tanımlanması

Year 2020, Volume: 6 Issue: 2, 302 - 309, 21.07.2020
https://doi.org/10.24180/ijaws.680153

Abstract

Yumrulu (terrestrial) orkideler, doğaya sayılamayacak kadar çok tohum bırakırlar. Orkide tohumlarında besi doku yoktur ve normal şartlarda çimlenmezler. Bu nedenle üretilemeyen türler arasındadır ve dünya genelinde koruma altına alınmıştır. Sadece mikorizalar ile simbiyotik ilişki kurabilen tohumlar çimlenebilirler. Çimlenen tohumların ise çiçek açması, tohum üretmesi uzun yıllar almaktadır. Bu çalışmada bazı salep türlerinin doğal ortamlarında çimlenmiş tohumları tespit edilmiş ve materyal olarak kullanılmıştır. Çimlenme yılı olarak tanımlanan ilk gelişim yılında, ele alınan türlerde, ilk yıl gelişim performansları yaprak sayısı, yaprak eni-boyu, yaş yumru eni-boyu, yaş yumru ağırlığı ve yaş biyokütle ağırlığı ile belirlenmiştir. Beş cinse ait sekiz türün incelendiği bu çalışmada çimlenme yılında 1-3 minik yaprak oluşumu gözlenmiş, toplam biyokütleleri ise bir gramdan daha az olmuştur. Geliştirdikleri yumruların taze ağırlıkları, türlere göre 0.06-0.31 gram arasında tespit edilmiştir. Veriler değerlendirildiğinde salep orkidelerinin çimlenme yılında minyatür bitkicikler olarak ilk yıl gelişimlerini tamamladıkları anlaşılmaktadır. Salep orkidelerinde, sorunlu çimlenme döneminden sonra, büyüme ve gelişmenin de yavaş gerçekleştiği görülmüştür.

References

  • Arabacı, O., Tutar, M., Öğretmen, N.G., Yasar, F., & Tan, U. (2014). Effects of different cultural practices in salep orchids. II. Medicinal and Aromatic Plants Symposium Proceeding Books, Yalova.
  • Arditti, J. (1992). Fundamentals of Orchid Biology. John Wiley and Sons, New York, USA.
  • Arditti, J., & Ghani, A. K. (2000). Numerical and physical properties of orchid seeds and their biyological implications. New Phytologist, 145, 367-421.
  • Attri, L.A. 2016. Therpeutic potential of orchids. World Journal of Pharmacy and Pharmaceutical Sciences, 5(2), 438-446.
  • Çığ, A., Demirer, D., & İşler, S. (2018). In vitro symbiotic germination potentials of some Anacamptis, Dactylorhiza, Orchis and Ophrys terrestrial orchid species. Applied Ecology and Environmental Research, 16(4), 5141-5155.
  • Bektaş, E., Cüce, M., & Sökmen, A. (2013). In vitro germination, protocorm formation, and plantlet development of Orchis coriophora (Orchidaceae), a naturally growing orchid species in Turkey. Turkish Journal of Botany, 37, 336-342.
  • Bektaş, E., & Sökmen, A. (2016). In vitro seed germination, plantlet growth, tuberization, and synthetic seed production of Serapias vomeracea (Burm.f.) Briq. Turkish Journal of Botany, 40, 584-594.
  • Boyraz, M., Korkmaz, H., & Durmaz, A. (2019). Dormancy and germination on seeds. Black Sea Journal of Engineering and Science, 2(3), 92-105.
  • Bulpitt, C. J. (2005). The uses and misuses of orchids in medicine. QJM An International Journal of Medicine, 98(9), 625-631.
  • Çalışkan, Ö. (2018). Salep Orkideleri. Erol Ofset Yayınları, Samsun.
  • Çalışkan, Ö. (2019). Middle Black Sea Region sahlep orchid species and tuber properties. Anadolu Tarım Bilimleri Dergisi, 34(1), 78-83.
  • Çalışkan, Ö., & Kurt, D. (2019). Dactylorhiza romana (sebast.) soo. salep orkidelerinde dört yıllık gelişim sürecinin tanımlanması. Anadolu Tarım Bilimleri Dergisi, 34, 195-200.
  • De, L.C., & Pathak, P. (2018). Conservation, management and utilization of orchid genetic resources. The Journal of the Orchid Society of India, 32, 81-91.
  • Delforge, P. (2006). Orchids of Europe, North Africa and the Middle East. Timber Press, Portland.
  • Endersby, J. (2016). Orchid: A Cultural History. Royal Botanic Garden Kew, London, UK.
  • Ertas, S., Ozel, A., & Erden, K. (2019). Determination of the botanical properties and glucomannan contents of some salep species cultivated in Şanlıurfa conditions. Harran Journal of Agricultural and Food Science, 23(1), 39-46.
  • Erzurumlu, G.S., & Doran, İ. (2011). Türkiye’de salep orkideleri ve salep kültürü. Harran Üniversitesi Ziraat Fakültesi Dergisi, 15(1), 29-34.
  • Giri, D., & Tamta, S. (2012). Propagation and conservation of Dactylorhiza hatagirea (D. Don) Soo, an endangered alpine orchid. African Journal of Biotechnology, 11(62), 12586-12594.
  • Ghorbani, A., Gravendeel, B., Zarre, S., & Boer, H. (2014). İllegal wild collection and international trade of Cites listed terretrial orchid tubers in Iran. Traffic Bulletin, 26(2), 53-58.
  • Govaerts, R., Bernet, P., Kratochvil, K., Gerlach, G., Carr, G., Alrich, P., Pridgeon, A. M., Pfahl, J., Campacci, M. A., Holland Baptista, D., Tigges, H., Shaw, J., Cribb, P., George, A., Kreuz, K., & Wood. J. J. (2017). World Checklist of Orchidaceae. Royal Botanic Gardens, Kew, London, UK.
  • Gümüş, C. (2009). Batı Karadeniz bölgesinde salep elde edilmesinde kullanılan bazı orkide türlerinin çoğaltım yöntemleri üzerine araştırmalar. Doktora tezi, Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Ankara.
  • Guler, N. (2005). Morphological and chorological studies on the orchids growing in Kazdağları. PhD Dissertation, Trakya University, Graduate School of Sciences, Tekirdağ.
  • Herrera, H., Valadares, R., Contreras, D., Bashan, Y., & Arriagada, C. (2017). Mycorrhizal compatibility and symbiotic seed germination of orchids from the Coastal Range and Andes in south central Chile. Mycorrhiza, 27, 175-188.
  • Hossain, M.M. (2011). Therapeutic orchids: traditional uses and recent advances-an overview. Fitoterapia, 82, 102-140.
  • Hughes, O.T.W. (2018). Orchid – mycorrhiza relationships, propagation of terrestrial and epiphytic orchids from seed. Doctoral thesis (PhD), Metropolitan University, Graduate School of Sciences, Manchester.
  • Jalal, J.S., Kumar, P., & Pangtey, Y.P.S. (2008). Ethnomedicinal orchids of Uttarakhand, Western Himalaya. Ethnobotanical Leaflets, 12, 1227-1230.
  • Kauth, P. (2005). In vitro seed germination and seedling development of Calopogon tuberosus and Sacoila lanceolata var. lanceolata: two florida native terrestrial orchids. Master Thesis, University of Florida, Graduate School of Sciences, Florida.
  • Kalimuthu, K., Senthilikumar, R., & Vijayakumar, S. (2007). In vitro micropropagation of orchid, Oncidum sp. African Journal of Biotechnology, 6(10), 1171-1174.
  • Kasparek, M., & Grim, U. (1999). European trade inTurkish salep with special reference to Germany. Economic Botany, 53(4), 396-406.
  • Kayıkçı, S., & Oğur, E. (2012). Hatay ilinde yayılış gösteren bazı orkide türleri üzerine bir inceleme. Anadolu Ege Tarımsal Araştırma Enstitüsü Dergisi, 22(2), 1-12.
  • Kreziou, A., De Boer, H., & Gravendeel, B. (2016). Harvesting of salep orchids in north-western Greece continues to threaten natural populations. Oryx, Fauna & Flora International, 50(3), 393-396.
  • Pant, B. (2013). Medicinal orchids and their uses: Tissue culture a potential alternative for conservation. African Journal of Plant Science, 7(10), 448-467.
  • Pierik, R. L. M. (1997). In vitro culture of higher plants. Kluwer academic Publisher, Wageningen Agricultural University, Netherlands.
  • Rasmussen, H. N. (1992). Seed dormancy patterns in Epipactis palustris (Orchidaceae): Requirements for germination and establishment of mycorrhiza. Physiologia Plantarum, 86, 161-167.
  • Rasmussen, H. N. (1995). Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge, UK.
  • Rasmussen, H. N. (2002). Recent developments in the study of orchid mycorrhiza. Plant and Soil, 244, 149-163.
  • Rasmussen, H. N., Dixon, K. W., Jersakova, J., & Tesitelova, T. (2015). Germination and seedling establishment in orchids: a complex of requirements. Annals of Botany, 116, 391-402.
  • Sandal, G. (2009). Eastern Mediterranean region and grows orchids with the threat of habitat characteristics to investigate factors. PhD Dissertation, Çukurova University, Graduate School of Sciences, Adana.
  • Sevgi, E., Altundag, E., Kara, O., Sevgi, O., Tecimen, H. B., & Bolat, I. (2012). Relations between morphological characteristics and community structure of Serapias bergonii E.G. Camus species in Çanakkale-Biga. Turkey II. Orchid and Salep Workshop Proceedings Book, Aegean Agricultural Research Institue, Izmir.
  • Sezik, E. (1984). Orkidelerimiz Türkiye’nin Orkideleri. Sandoz Kültür Yayınları, No: 6, İstanbul.
  • Szendrak, E. (1997). Asymbiotic in vitro seed germination, micropropagation and scanning electron microscopy of several temperate terrestrial orchids (Orchidaceae). PhD thesis, University of Nebraska, Graduate School of Sciences, Lincoln.
  • Şen, M.A. (2017). The forbidden treasure from soil to cone: Salep. Journal of Food Engineering, 42, 65-69.
  • Tutar, M., Parlak, S., Sarı, A.O., & Çiçek, F. (2013;). Salep orkidelerinde tohumdan üretim. XI. Ulusal Ekoloji ve Çevre Kongresi, Samsun.
  • Utami, E.S.W., Hariyanto, S., & Manuhara, Y.S.W. (2017). In vitro propagation of the endangered medicinal orchid, Dendrobium lasianthera J.J.Sm through mature seed culture. Asian Pacific Journal of Tropical Biomedicine, 7(5), 406-410.
  • Warghat, A.R., Bajpai, P.K., Srivastava, R.B., Chaurasia, P., Chauhan, R.S., & Sood, H. (2014). In vitro protocorm development and mass multiplication of an endangered orchid, D. hatagirea. Turkish Journal of Botany, 38, 737-746.
  • Whigham, D.F. & Willems, J.H. (2003). Demographic studies and life-history strategies of temperate terrestrial orchids as a basis for conservation. In W. Kingsley Dixon, P. Shelagh, R. L. Barrett & Philip Cribb (Eds.), Orchid conservation (pp. 137-158). Kota Kinabaluk Sabah, Malaysia.

Description of the First Year's Development of Eight Different Species of Salep Orchids Germinated in Natural Flora

Year 2020, Volume: 6 Issue: 2, 302 - 309, 21.07.2020
https://doi.org/10.24180/ijaws.680153

Abstract

Tuberous (terrestrial) orchids leave too many seeds to nature. Orchid seeds do not have endosperm and cannot germinate under normal conditions. For this reason, they are among the species that cannot be produced and it is protected worldwide. Seeds that can form symbiotic relationships with mycorrhiza can only germinate. It takes many years for germinated seeds to inflorescence and produce seeds. In this study, germinated seeds of some salep species in the natural flora were used as material. In the first year of development defined as the germination year, the developmental performances of the species were determined by measuring the number of leaves, leaf width, leaf lenght, tuber width, tuber lenght, fresh tuber weight and fresh biomass weight. In this study, in which eight species belonging to five genera were examined, 1-3 small leaf formation was observed in the germination year, and total fresh biomass was less than one gram. The fresh weights of the tubers were determined between 0.06-0.31 grams according to the species. When the data are evaluated, it is understood that salep orchids have completed their first year development as miniature plants in the germination year. It was observed that growth and development were slow, after the problematic germination period, in salep orchids.

References

  • Arabacı, O., Tutar, M., Öğretmen, N.G., Yasar, F., & Tan, U. (2014). Effects of different cultural practices in salep orchids. II. Medicinal and Aromatic Plants Symposium Proceeding Books, Yalova.
  • Arditti, J. (1992). Fundamentals of Orchid Biology. John Wiley and Sons, New York, USA.
  • Arditti, J., & Ghani, A. K. (2000). Numerical and physical properties of orchid seeds and their biyological implications. New Phytologist, 145, 367-421.
  • Attri, L.A. 2016. Therpeutic potential of orchids. World Journal of Pharmacy and Pharmaceutical Sciences, 5(2), 438-446.
  • Çığ, A., Demirer, D., & İşler, S. (2018). In vitro symbiotic germination potentials of some Anacamptis, Dactylorhiza, Orchis and Ophrys terrestrial orchid species. Applied Ecology and Environmental Research, 16(4), 5141-5155.
  • Bektaş, E., Cüce, M., & Sökmen, A. (2013). In vitro germination, protocorm formation, and plantlet development of Orchis coriophora (Orchidaceae), a naturally growing orchid species in Turkey. Turkish Journal of Botany, 37, 336-342.
  • Bektaş, E., & Sökmen, A. (2016). In vitro seed germination, plantlet growth, tuberization, and synthetic seed production of Serapias vomeracea (Burm.f.) Briq. Turkish Journal of Botany, 40, 584-594.
  • Boyraz, M., Korkmaz, H., & Durmaz, A. (2019). Dormancy and germination on seeds. Black Sea Journal of Engineering and Science, 2(3), 92-105.
  • Bulpitt, C. J. (2005). The uses and misuses of orchids in medicine. QJM An International Journal of Medicine, 98(9), 625-631.
  • Çalışkan, Ö. (2018). Salep Orkideleri. Erol Ofset Yayınları, Samsun.
  • Çalışkan, Ö. (2019). Middle Black Sea Region sahlep orchid species and tuber properties. Anadolu Tarım Bilimleri Dergisi, 34(1), 78-83.
  • Çalışkan, Ö., & Kurt, D. (2019). Dactylorhiza romana (sebast.) soo. salep orkidelerinde dört yıllık gelişim sürecinin tanımlanması. Anadolu Tarım Bilimleri Dergisi, 34, 195-200.
  • De, L.C., & Pathak, P. (2018). Conservation, management and utilization of orchid genetic resources. The Journal of the Orchid Society of India, 32, 81-91.
  • Delforge, P. (2006). Orchids of Europe, North Africa and the Middle East. Timber Press, Portland.
  • Endersby, J. (2016). Orchid: A Cultural History. Royal Botanic Garden Kew, London, UK.
  • Ertas, S., Ozel, A., & Erden, K. (2019). Determination of the botanical properties and glucomannan contents of some salep species cultivated in Şanlıurfa conditions. Harran Journal of Agricultural and Food Science, 23(1), 39-46.
  • Erzurumlu, G.S., & Doran, İ. (2011). Türkiye’de salep orkideleri ve salep kültürü. Harran Üniversitesi Ziraat Fakültesi Dergisi, 15(1), 29-34.
  • Giri, D., & Tamta, S. (2012). Propagation and conservation of Dactylorhiza hatagirea (D. Don) Soo, an endangered alpine orchid. African Journal of Biotechnology, 11(62), 12586-12594.
  • Ghorbani, A., Gravendeel, B., Zarre, S., & Boer, H. (2014). İllegal wild collection and international trade of Cites listed terretrial orchid tubers in Iran. Traffic Bulletin, 26(2), 53-58.
  • Govaerts, R., Bernet, P., Kratochvil, K., Gerlach, G., Carr, G., Alrich, P., Pridgeon, A. M., Pfahl, J., Campacci, M. A., Holland Baptista, D., Tigges, H., Shaw, J., Cribb, P., George, A., Kreuz, K., & Wood. J. J. (2017). World Checklist of Orchidaceae. Royal Botanic Gardens, Kew, London, UK.
  • Gümüş, C. (2009). Batı Karadeniz bölgesinde salep elde edilmesinde kullanılan bazı orkide türlerinin çoğaltım yöntemleri üzerine araştırmalar. Doktora tezi, Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Ankara.
  • Guler, N. (2005). Morphological and chorological studies on the orchids growing in Kazdağları. PhD Dissertation, Trakya University, Graduate School of Sciences, Tekirdağ.
  • Herrera, H., Valadares, R., Contreras, D., Bashan, Y., & Arriagada, C. (2017). Mycorrhizal compatibility and symbiotic seed germination of orchids from the Coastal Range and Andes in south central Chile. Mycorrhiza, 27, 175-188.
  • Hossain, M.M. (2011). Therapeutic orchids: traditional uses and recent advances-an overview. Fitoterapia, 82, 102-140.
  • Hughes, O.T.W. (2018). Orchid – mycorrhiza relationships, propagation of terrestrial and epiphytic orchids from seed. Doctoral thesis (PhD), Metropolitan University, Graduate School of Sciences, Manchester.
  • Jalal, J.S., Kumar, P., & Pangtey, Y.P.S. (2008). Ethnomedicinal orchids of Uttarakhand, Western Himalaya. Ethnobotanical Leaflets, 12, 1227-1230.
  • Kauth, P. (2005). In vitro seed germination and seedling development of Calopogon tuberosus and Sacoila lanceolata var. lanceolata: two florida native terrestrial orchids. Master Thesis, University of Florida, Graduate School of Sciences, Florida.
  • Kalimuthu, K., Senthilikumar, R., & Vijayakumar, S. (2007). In vitro micropropagation of orchid, Oncidum sp. African Journal of Biotechnology, 6(10), 1171-1174.
  • Kasparek, M., & Grim, U. (1999). European trade inTurkish salep with special reference to Germany. Economic Botany, 53(4), 396-406.
  • Kayıkçı, S., & Oğur, E. (2012). Hatay ilinde yayılış gösteren bazı orkide türleri üzerine bir inceleme. Anadolu Ege Tarımsal Araştırma Enstitüsü Dergisi, 22(2), 1-12.
  • Kreziou, A., De Boer, H., & Gravendeel, B. (2016). Harvesting of salep orchids in north-western Greece continues to threaten natural populations. Oryx, Fauna & Flora International, 50(3), 393-396.
  • Pant, B. (2013). Medicinal orchids and their uses: Tissue culture a potential alternative for conservation. African Journal of Plant Science, 7(10), 448-467.
  • Pierik, R. L. M. (1997). In vitro culture of higher plants. Kluwer academic Publisher, Wageningen Agricultural University, Netherlands.
  • Rasmussen, H. N. (1992). Seed dormancy patterns in Epipactis palustris (Orchidaceae): Requirements for germination and establishment of mycorrhiza. Physiologia Plantarum, 86, 161-167.
  • Rasmussen, H. N. (1995). Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge, UK.
  • Rasmussen, H. N. (2002). Recent developments in the study of orchid mycorrhiza. Plant and Soil, 244, 149-163.
  • Rasmussen, H. N., Dixon, K. W., Jersakova, J., & Tesitelova, T. (2015). Germination and seedling establishment in orchids: a complex of requirements. Annals of Botany, 116, 391-402.
  • Sandal, G. (2009). Eastern Mediterranean region and grows orchids with the threat of habitat characteristics to investigate factors. PhD Dissertation, Çukurova University, Graduate School of Sciences, Adana.
  • Sevgi, E., Altundag, E., Kara, O., Sevgi, O., Tecimen, H. B., & Bolat, I. (2012). Relations between morphological characteristics and community structure of Serapias bergonii E.G. Camus species in Çanakkale-Biga. Turkey II. Orchid and Salep Workshop Proceedings Book, Aegean Agricultural Research Institue, Izmir.
  • Sezik, E. (1984). Orkidelerimiz Türkiye’nin Orkideleri. Sandoz Kültür Yayınları, No: 6, İstanbul.
  • Szendrak, E. (1997). Asymbiotic in vitro seed germination, micropropagation and scanning electron microscopy of several temperate terrestrial orchids (Orchidaceae). PhD thesis, University of Nebraska, Graduate School of Sciences, Lincoln.
  • Şen, M.A. (2017). The forbidden treasure from soil to cone: Salep. Journal of Food Engineering, 42, 65-69.
  • Tutar, M., Parlak, S., Sarı, A.O., & Çiçek, F. (2013;). Salep orkidelerinde tohumdan üretim. XI. Ulusal Ekoloji ve Çevre Kongresi, Samsun.
  • Utami, E.S.W., Hariyanto, S., & Manuhara, Y.S.W. (2017). In vitro propagation of the endangered medicinal orchid, Dendrobium lasianthera J.J.Sm through mature seed culture. Asian Pacific Journal of Tropical Biomedicine, 7(5), 406-410.
  • Warghat, A.R., Bajpai, P.K., Srivastava, R.B., Chaurasia, P., Chauhan, R.S., & Sood, H. (2014). In vitro protocorm development and mass multiplication of an endangered orchid, D. hatagirea. Turkish Journal of Botany, 38, 737-746.
  • Whigham, D.F. & Willems, J.H. (2003). Demographic studies and life-history strategies of temperate terrestrial orchids as a basis for conservation. In W. Kingsley Dixon, P. Shelagh, R. L. Barrett & Philip Cribb (Eds.), Orchid conservation (pp. 137-158). Kota Kinabaluk Sabah, Malaysia.
There are 46 citations in total.

Details

Primary Language Turkish
Journal Section Field Crops
Authors

Dursun Kurt 0000-0001-6697-3954

Ömer Çalışkan 0000-0003-2235-3184

Publication Date July 21, 2020
Submission Date January 26, 2020
Acceptance Date May 29, 2020
Published in Issue Year 2020 Volume: 6 Issue: 2

Cite

APA Kurt, D., & Çalışkan, Ö. (2020). Doğal Florada Çimlenmiş Sekiz Farklı Salep Orkidesi Türünün İlk Yıl Gelişimlerinin Tanımlanması. International Journal of Agricultural and Wildlife Sciences, 6(2), 302-309. https://doi.org/10.24180/ijaws.680153

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