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Yenilebilir Hercai Menekşe Çiçeğinin (Viola × wittrockiana) Biyoaktif bileşikleri Üzerine Bir Etilen İnhibitörü Aminoethoxyvinylglycine (AVG)’in Etkisi

Year 2025, Volume: 11 Issue: 2, 172 - 177, 29.08.2025
https://doi.org/10.24180/ijaws.1704379

Abstract

Yenilebilir çiçekler tüketiciler tarafından salata, tatlı, içecek ve garnitür gibi yemeklerde besin takviyesi olarak kullanılmaktadır. Ayrıca bunlar insan sağlığına zararlı olmayan ve besin değeri yüksek gıdalardır. Yenilebilir çiçekler vitamin, mineral, fenolik bileşikler ve yüksek antioksidan kapasiteye sahip gıdalardır ve yemeklere görsel çekicilik ve çeşitlilik katarlar. Bu çalışmada, etilen inhibitörü aminoetoksivinilglisinin (AVG) yenilebilir hercai menekşelerin biyoaktif bileşenleri üzerindeki etkisi araştırılmıştır. Çalışmada uygulama olarak kontrol (saf su), 250 µmol AVG ve 500 µmol AVG dozları kullanılmıştır. Uygulamalar, yapraktan püskürtme (sprey) yöntemi ile gerçekleştirilmiştir. Çalışmada, toplam fenolik içeriği bakımından uygulamalar arasında istatistiksel olarak anlamlı bir farkın olmadığı belirlenmiştir. Ancak, AVG uygulanan çiçeklerin flavonoid içeriğinin, kontrol grubuna kıyasla daha düşük olduğu tespit edilmiştir. Bununla birlikte, toplam antosiyanin içeriği AVG uygulamaları ile anlamlı düzeyde artmıştır. Nitekim en yüksek değerler sırasıyla 0.66 ve 0.74 g cy-3 kg⁻¹ ile 250 µmol ve 500 µmol AVG uygulamalarında elde edilmiştir. DPPH testine göre, AVG uygulanan çiçeklerde antioksidan aktivite kontrol grubuna göre daha düşük bulunmuştur. Ancak, FRAP testine göre 500 µmol AVG uygulaması antioksidan aktiviteyi anlamlı şekilde artırmıştır. 250 µmol AVG uygulamasında, kateşin, klorojenik asit ve epikateşin içerikleri diğer uygulamalara kıyasla istatistiki açından önemli düzeyde daha yüksek bulunurken, 500 µmol AVG uygulamasında ise rutin içeriği önemli şekilde daha yüksek olduğu tespit edilmiştir. Sonuç olarak, çalışma AVG uygulamasının hercai menekşe çiçeklerinin fitokimyasal içeriği üzerinde etkili olduğunu ve bu etkinin doza göre değiştiğini ortaya koymuştur.

References

  • Al-Zughbi, I., & Krayem, M. (2022). Quince fruit Cydonia oblonga Mill nutritional composition, antioxidative properties, health benefits and consumers preferences towards some industrial quince products: A review. Food Chemistry, 393, 133362. https://doi.org/10.1016/j.foodchem.2022.133362
  • Aglar, E., Ozturk, B., Guler, S. K., Karakaya, O., Uzun, S., & Saracoglu, O. (2017). Effect of modified atmosphere packaging and ‘Parka’ treatments on fruit quality characteristics of sweet cherry fruits (Prunus avium L.‘0900 Ziraat’) during cold storage and shelf life. Scientia Horticulturae, 222, 162-168. https://doi.org/10.1016/j.scienta.2017.05.024
  • Aglar, E. (2023). Effects of aminoethoxyvinylglycine (AVG) on fruit quality and bioactive content of jujube fruit (Ziziphus jujuba) harvested at three maturity stages during cold storage. Erwerbs-Obstbau, 65(4), 879-888. https://doi.org/10.1007/s10341-022-00752-0
  • Babalık, Z. (2021). Effect of aminoethoxyvinylglycine (AVG) on quality, berry set and coloration of ‘Alphonse Lavallée’ table grapes. The Journal of Agricultural Science, 159(3-4), 236-242. https://doi.org/10.1017/S0021859621000496
  • Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1), 70-76. https://doi.org/10.1006/abio.1996.0292
  • Chang, C. C., Yang, M. H., Wen, H. M., & Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), 178-182.
  • da Silva, L. A., Fischer, S. Z., & Zambiazi, R. C. (2020). Proximal composition, bioactive compounds content and color preference of Viola x wittrockiana flowers. International Journal of Gastronomy and Food Science, 22, 100236. https://doi.org/10.1016/j.ijgfs.2020.100236
  • Fadhil, N. N., & Al-Bamarny, S. F. (2009, April 8-12). Postharvest Aminoethoxyvinylglycine (Avg) Dips on Storage Characteristics of 'golden Delicious' and 'red Delicious' apples [Paper presentation]. In VI International Postharvest Symposium, Türkiye.
  • Fernandes, L., Ramalhosa, E., Baptista, P., Pereira, J. A., Saraiva, J. A., & Casal, S. I. (2019). Nutritional and nutraceutical composition of pansies (Viola × wittrockiana) during flowering. Journal of Food Science, 84(3), 490-498. https://doi.org/10.1111/1750-3841.14482
  • Giusti, M. M., & Wrolstad, R. E. (2001). Characterization and measurement of anthocyanins by UV‐visible spectroscopy. Current Protocols in Food Analytical Chemistry, 1, F1-2. https://doi.org/10.1002/0471142913.faf0102s00
  • Khan, A. S., Singh, Z., & Abbasi, N. A. (2007). Pre-storage putrescine application suppresses ethylene biosynthesis and retards fruit softening during low temperature storage in ‘Angelino’ plum. Postharvest Biology and Technology, 46(1), 36-46. https://doi.org/10.1016/j.postharvbio.2007.03.018
  • Karaman, S., Ozturk, B., Aksit, H., & Erdogdu, T. (2013). The effects of pre-harvest application of aminoethoxyvinylglycine on the bioactive compounds and fruit quality of ‘Fortune’ plum variety during cold storage. Food Science and Technology International, 19(6), 567-576. https://doi.org/10.1177/1082013212457668
  • Karakaya, O., Öztürk, B., Ağlar, E., & Balık, H. İ. (2021). The Influence of the Rootstocks on Biochemical and Bioactive Compound Content of '0900 Ziraat' Sweet Cherry Fruit. Erwerbs-Obstbau, 63, 247–253, https://doi.org/10.1007/s10341-021-00542-0
  • Kucuker, E., Ozturk, B., Yildiz, K., & Ozkan, Y. (2015). Effect of aminoethoxyvinylglycine (AVG) on the quality of Japanese plum (Prunus salicina Lindell cv. Fortune) fruits. Acta Scientiarum Polonorum Hortorum Cultus, 14(5), 3-17.
  • Lim, T. K. (2012). Edible medicinal and non-medicinal plants. Dordrecht, The Netherlands, Springer.
  • Peng, C. H., Chyau, C. C., Chan, K. C., Chan, T. H., Wang, C. J., & Huang, C. N. (2011). Hibiscus sabdariffa polyphenolic extract inhibits hyperglycemia, hyperlipidemia, and glycation-oxidative stress while improving insulin resistance. Journal of Agricultural and Food Chemistry, 59(18), 9901-9909. https://doi.org/10.1021/jf2022379
  • Rop, O., Mlcek, J., Jurikova, T., Neugebauerova, J., & Vabkova, J. (2012). Edible flowers-a new promising source of mineral elements in human nutrition. Molecules, 17(6), 6672-6683. https://doi.org/10.3390/molecules17066672
  • Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144-158. http://doi.org/10.5344/ajev.1965.16.3.144
  • Yildiz, K., Kilic, K., Ozkan, Y., Ozturk, B., & Kucuker, E. (2018). The role of pre-harvest aminoethoxyvinylglycine (AVG) treatments on total phenolics, antioxidant capacity and fruit quality attributes of sweet cherry cultivars. Erwerbs-Obstbau, 60(3), 221-230. https://doi.org/10.1007/s10341-017-0362-6
  • Villavicencio, A. L., Heleno, S. A., Calhelha, R. C., Santos-Buelga, C., Barros, L., & Ferreira, I. C. (2018). The influence of electron beam radiation in the nutritional value, chemical composition and bioactivities of edible flowers of Bauhinia variegata L. var. candida alba Buch.-Ham from Brazil. Food Chemistry, 241, 163-170. https://doi.org/10.1016/j.foodchem.2017.08.093

Effect of Aminoethoxyvinylglycine (AVG) as an Ethylene Inhibitor on Bioactive Compounds of Edible Pansy Flowers (Viola × wittrockiana)

Year 2025, Volume: 11 Issue: 2, 172 - 177, 29.08.2025
https://doi.org/10.24180/ijaws.1704379

Abstract

Edible flowers are used by consumers as a dietary supplement in dishes such as salads, desserts, drinks and garnitures. They are foods that are not harmful to human health and have a high nutritional value. Edible flowers are foods with vitamins, minerals, phenolic compounds and high antioxidant capacity, and they add visual appeal and variety to dishes. In this study, the effect of the ethylene inhibitor aminoethoxyvinylglycine (AVG) on the bioaktive compounds of edible pansies was investigated. In the study, the treatments included control (distilled water), 250 µmol AVG, and 500 µmol AVG. The treatments were applied to the plants as a foliar spray. Measurements were made before and 5 days after application of AVG. In the study, no statistically significant difference was found between the treatments in terms of total phenolic content. However, the flavonoid content of flowers treated with AVG was found to be lower compared to the control treatment. On the other hand, total anthocyanin content significantly increased with AVG treatments. Indeed, the highest values were obtained with 250 µmol and 500 µmol AVG treatments, measuring 0.66 and 0.74 g cy-3 kg⁻¹, respectively. According to the DPPH test, the antioxidant activity of AVG-treated flowers was lower than that of the control group. However, the FRAP test showed that the 500 µmol AVG treatment significantly enhanced antioxidant activity. In the 250 µmol AVG treatment, catechin, chlorogenic acid, and epicatechin contents were found to be significantly higher compared to the other treatments, while in the 500 µmol AVG treatment, rutin content was determined to be significantly higher. As a result, the study revealed that AVG application has an effect on the phytochemical content of pansy flowers, and this effect varies according to the dose.

References

  • Al-Zughbi, I., & Krayem, M. (2022). Quince fruit Cydonia oblonga Mill nutritional composition, antioxidative properties, health benefits and consumers preferences towards some industrial quince products: A review. Food Chemistry, 393, 133362. https://doi.org/10.1016/j.foodchem.2022.133362
  • Aglar, E., Ozturk, B., Guler, S. K., Karakaya, O., Uzun, S., & Saracoglu, O. (2017). Effect of modified atmosphere packaging and ‘Parka’ treatments on fruit quality characteristics of sweet cherry fruits (Prunus avium L.‘0900 Ziraat’) during cold storage and shelf life. Scientia Horticulturae, 222, 162-168. https://doi.org/10.1016/j.scienta.2017.05.024
  • Aglar, E. (2023). Effects of aminoethoxyvinylglycine (AVG) on fruit quality and bioactive content of jujube fruit (Ziziphus jujuba) harvested at three maturity stages during cold storage. Erwerbs-Obstbau, 65(4), 879-888. https://doi.org/10.1007/s10341-022-00752-0
  • Babalık, Z. (2021). Effect of aminoethoxyvinylglycine (AVG) on quality, berry set and coloration of ‘Alphonse Lavallée’ table grapes. The Journal of Agricultural Science, 159(3-4), 236-242. https://doi.org/10.1017/S0021859621000496
  • Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1), 70-76. https://doi.org/10.1006/abio.1996.0292
  • Chang, C. C., Yang, M. H., Wen, H. M., & Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), 178-182.
  • da Silva, L. A., Fischer, S. Z., & Zambiazi, R. C. (2020). Proximal composition, bioactive compounds content and color preference of Viola x wittrockiana flowers. International Journal of Gastronomy and Food Science, 22, 100236. https://doi.org/10.1016/j.ijgfs.2020.100236
  • Fadhil, N. N., & Al-Bamarny, S. F. (2009, April 8-12). Postharvest Aminoethoxyvinylglycine (Avg) Dips on Storage Characteristics of 'golden Delicious' and 'red Delicious' apples [Paper presentation]. In VI International Postharvest Symposium, Türkiye.
  • Fernandes, L., Ramalhosa, E., Baptista, P., Pereira, J. A., Saraiva, J. A., & Casal, S. I. (2019). Nutritional and nutraceutical composition of pansies (Viola × wittrockiana) during flowering. Journal of Food Science, 84(3), 490-498. https://doi.org/10.1111/1750-3841.14482
  • Giusti, M. M., & Wrolstad, R. E. (2001). Characterization and measurement of anthocyanins by UV‐visible spectroscopy. Current Protocols in Food Analytical Chemistry, 1, F1-2. https://doi.org/10.1002/0471142913.faf0102s00
  • Khan, A. S., Singh, Z., & Abbasi, N. A. (2007). Pre-storage putrescine application suppresses ethylene biosynthesis and retards fruit softening during low temperature storage in ‘Angelino’ plum. Postharvest Biology and Technology, 46(1), 36-46. https://doi.org/10.1016/j.postharvbio.2007.03.018
  • Karaman, S., Ozturk, B., Aksit, H., & Erdogdu, T. (2013). The effects of pre-harvest application of aminoethoxyvinylglycine on the bioactive compounds and fruit quality of ‘Fortune’ plum variety during cold storage. Food Science and Technology International, 19(6), 567-576. https://doi.org/10.1177/1082013212457668
  • Karakaya, O., Öztürk, B., Ağlar, E., & Balık, H. İ. (2021). The Influence of the Rootstocks on Biochemical and Bioactive Compound Content of '0900 Ziraat' Sweet Cherry Fruit. Erwerbs-Obstbau, 63, 247–253, https://doi.org/10.1007/s10341-021-00542-0
  • Kucuker, E., Ozturk, B., Yildiz, K., & Ozkan, Y. (2015). Effect of aminoethoxyvinylglycine (AVG) on the quality of Japanese plum (Prunus salicina Lindell cv. Fortune) fruits. Acta Scientiarum Polonorum Hortorum Cultus, 14(5), 3-17.
  • Lim, T. K. (2012). Edible medicinal and non-medicinal plants. Dordrecht, The Netherlands, Springer.
  • Peng, C. H., Chyau, C. C., Chan, K. C., Chan, T. H., Wang, C. J., & Huang, C. N. (2011). Hibiscus sabdariffa polyphenolic extract inhibits hyperglycemia, hyperlipidemia, and glycation-oxidative stress while improving insulin resistance. Journal of Agricultural and Food Chemistry, 59(18), 9901-9909. https://doi.org/10.1021/jf2022379
  • Rop, O., Mlcek, J., Jurikova, T., Neugebauerova, J., & Vabkova, J. (2012). Edible flowers-a new promising source of mineral elements in human nutrition. Molecules, 17(6), 6672-6683. https://doi.org/10.3390/molecules17066672
  • Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144-158. http://doi.org/10.5344/ajev.1965.16.3.144
  • Yildiz, K., Kilic, K., Ozkan, Y., Ozturk, B., & Kucuker, E. (2018). The role of pre-harvest aminoethoxyvinylglycine (AVG) treatments on total phenolics, antioxidant capacity and fruit quality attributes of sweet cherry cultivars. Erwerbs-Obstbau, 60(3), 221-230. https://doi.org/10.1007/s10341-017-0362-6
  • Villavicencio, A. L., Heleno, S. A., Calhelha, R. C., Santos-Buelga, C., Barros, L., & Ferreira, I. C. (2018). The influence of electron beam radiation in the nutritional value, chemical composition and bioactivities of edible flowers of Bauhinia variegata L. var. candida alba Buch.-Ham from Brazil. Food Chemistry, 241, 163-170. https://doi.org/10.1016/j.foodchem.2017.08.093
There are 20 citations in total.

Details

Primary Language English
Subjects Horticultural Production (Other)
Journal Section Research Article
Authors

Sefa Gün 0000-0002-9516-386X

Burhan Öztürk 0000-0002-0867-3942

Can Alper Hekimoglu 0009-0004-2809-8545

Early Pub Date August 28, 2025
Publication Date August 29, 2025
Submission Date May 22, 2025
Acceptance Date July 23, 2025
Published in Issue Year 2025 Volume: 11 Issue: 2

Cite

APA Gün, S., Öztürk, B., & Hekimoglu, C. A. (2025). Effect of Aminoethoxyvinylglycine (AVG) as an Ethylene Inhibitor on Bioactive Compounds of Edible Pansy Flowers (Viola × wittrockiana). Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi, 11(2), 172-177. https://doi.org/10.24180/ijaws.1704379

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