Characterization and siderophores production of Rhizobium spp. isolated from wild legumes

Year 2020, Volume: 6 Issue: 3, 176 - 179, 30.11.2020

Hatice Öğütcü Hulya Avsar

Abstract

Legume plants are very significant not only ecologically but also agriculturally because they are responsible for major change of nitrogen from atmospheric N2 to ammonia. In this study, total 56 isolates of Rhizobium spp. which were previously isolated from wild legumes plant (Melilotus officinalis, Medicago sativa and Vicia cracca) existing in the central and the disricts of Kırşehir province (Kaman, Mucur, Akpınar, Akçakent, Çiçekdağı, Boztepe). In order to characterize the isolates; YMA containing Bromothymol blue, Congo red, Gram stain reaction , movement, catalase and oxidase tests were evaluated.
In addition, isolates of Rhizobium spp. (wild type) were screened for their ability to produce siderophores and it was determined that 50 of 56 isolates in total can produce siderophore.

Keywords

Rhizobium spp., Melilotus officinalis, Vicia cracca, Medicago sativa, Siderophore

Supporting Institution

KIRŞEHİR AHİ EVRAN UNİV.

Project Number

Ahi Evran Univ. BAP No: FEF.B2.16.004.

Thanks

This work was supported Ahi Evran Univ. BAP No: FEF.B2.16.004.

References

• [1] Teaumroong N., Boonkerd N., Detection of Bradyhizobium spp. and B. japonicum in Thailand by primer-based technology and direct DNA extraction. Plant Soil 204:127-134, (1998).
• [2] Rigby D., Caceres D., Organic farming and the sustainability of agricultural systems. Agricultural Systems 68:21-40. (2001). DOI: 10.1016/S0308-521X(00)00060-3.
• [3] Lee J.Y. and Song S. H., Evaluation of groundwater quality in coastal areas: implications for sustainable agriculture. Environmental Geology 52:1231-1242, (2007). DOI: 10.1007/s00254-006-0560-2.
• [4] Öğütçü H., Algur O. F., Elkoca E., Kantar F. The determination of symbiotic effectiveness of Rhizobium strains isolated from wild chickpea collected from high altitudes in Erzurum. Turkish Journal of Agriculture and Forestry 32:241-248, (2008). DOI: 10.3906/tar-0904-34.
• [5] Zahran H.H., Rhizobia from wild legumes: diversity, taxonomy, ecology, nitrogen fixation and biotechnology. Journal of Biotechnology 91:143-153, (2001). DOI: 10.1016/S0168-1656(01)00342-X.
• [6] Ögütcü H., Kasımoğlu C., Elkoca E., Effects of Rhizobium strains ısolated from wild chickpeas on the growth and symbiotic performance of chickpea (Cicer arietinum L.) under salt stress. Turk J Agric For 34: 361-371, (2010). DOI: 10.3906/tar-0904-34.
• [7] Adıguzel A., Ogutcu H., Baris O., Karadayi M., Gulluce M., Isolation and characterization of Rhizobium strains from wild vetch collected from high altitudes in Erzurum-Turkey. Romanian Biotechnological Letters 15(1) : 5017-5024, (2010).
• [8] Dhul M., Suneja S., Dadarwal K. R., Role of siderophores in chickpea (Cicer arietinum L.) – Rhizobium symbiosis. Microbiol. Res. 153:47-53, (1998). DOI: 10.1016/S0944-5013(98)80020-7.
• [9] Datta B., Chakrabartty P. K., Siderophore biosynthesis genes of Rhizobium sp. isolated from Cicer arietinum L. 3 Biotech 4:391–401,(2014). DOI 10.1007/s13205-013-0164-y.
• [10] Erdem B., Dayangaç A., Günaydin, M., Tulumoglu S., Yilmaz M., Effect of heavy metals and antibioticson siderophores producing bacterial isolates. Acta physica polonica A 130:181-183,(2016). DOI: 0.12693/APhysPolA.130.181.
• [11] Guerinot M.L., Iron uptake and metabolism in the rhizobia/ legume symbioses. Plant Soil 130:199–209, (1991).
• [12] Joshi F. R., Kholiya S. P., Archana G., Desai A. J. Siderophore cross-utilization amongst nodule isolates of the cowpea miscellany group and its effect on plant growth in the presence of antagonistic organisms. Microbiological Research 163: 564—570, (2008). DOI: 10.1016/j.micres.2006.08.004.
• [13] Vincent J.M., A Manual for the practical study of root nodule bacteria. Blackwell Scientific, Oxford, (1970).
• [14] Öğütçü H., Adıgüzel A., Güllüce M., Karadayı M., Şahin F., Molecular characterization of Rhizobium strains isolated from wild chickpeas collected from high altitudes in Erzurum-Turkey. Romanian Biotechnological Letters 14(2): 4294-4300, (2009).
• [15] Kantar F., Elkoca E., Öğütcü H., Algur Ö. F. Chickpea yields in relation to Rhizobium inoculation from wild chickpea at high altitudes. J Agronomy Crop Science 189: 291-297, (2003). DOI: 10.1046/j.1439-037X.2003.00046.x.
• [16] Schwyn B., Neilands J.B., Universal Chemical assay for the detection and determination of siderophores. Analytical Biochemistry 160: 47-56, (1987). DOI:10.1016/0003-2697(87)90612-9.
• [17] Van Rossum D., Muyotcha A., Van Verseveld H.W., Stouthamer A.H., Boogred F. C. Siderophore production by Bradyrhizobium spp. strains nodulating groundnut. Plant Soil 163: 177-187,(1994).
• [18] Tyagi A., Kumar V., Tomar P. and A. Isolation, Identification, Biochemical and Antibiotic Sensitivity Characterization of Rhizobium Strains from Vigna mungo(L) Hepper, Cicer arietinumL and Vigna radiata(L) R Wilczek in Muzaffarnagar, Uttar Pradesh, India. Int. J. Curr.Microbiol. App. Sci 6:2024-2035, (2017). DOI: 0.20546/ijcmas.2017.612.233.