The Effects of Biopesticide Azadirachtin on the Fifth Instar Galleria mellonella L. (Lepidoptera: Pyralidae) Larval Integument

Year 2016, Volume: 2 Issue: 2, 0 - 0, 07.07.2016

Sadettin Ünsal Emine Güner

Abstract

The chitin synthesis inhibitory (CSI) effects of azadirachtin on the fifth instar Galleria mellonella L. larval integument were investigated. Using probit analysis, the LC95 value for G. mellonella larvae was detected as 3991 ppm. In this study, 3991 ppm and the reduced 1995, 997, and 498 ppm concentrations were used. It was observed that azadirachtin had different effects on the integument when the larvae were fed semi-synthetic feed containing 498, 997, 1995, or 3991 ppm azadirachtin. Morphologically failed molting, disruption of cuticle structure, hemolymph loss, and darkening were observed in azadirachtin-administered larvae. In the experimental groups, depending on the dose/time relationship, high doses of azadirachtin blocked cuticle secretion in higher levels. In larvae fed a diet containing azadirachtin, cuticle secretion was reduced by 21%–24% compared with the control group.

References

• Adhikary, S., 1984. Results of field trials to control common insect. pests okra, Hibuscus esculentus L., in Togo by application of crude methanolic extracts of leaves and seed kernels of the neem tree, Azadirachta indica. A. Juss. Zeitsclirift für angewandte Entomologie, 98:4, 327-331.
• Abstracted in: Ent. Abst., (1985), 16 :5, 98, No:4480
• Arnason, J.T., Philogene, B.J.R., Donskov, N., Hudon, Mc Dougall, M.C.G., Fortier, P., Morand, D., Gardner and Lambert, J., 1985. Antifeedant and insecticidal properties of azadirachtin to the European corn borer, Ostrinia nubilatis. Entomologia Experimentalis et Applicata, 38:1, 29-34. Abstracted in: Ent. Abst. (1986), 17:1, 38-39, No: 295.
• Ascher, K.R.S., 1993. Nonconventional insecticidal effects of pesticides available from the neem tree, Azadirachta indica. Archives of Insect Biochemistry and physiology, 22: 433-449.
• Cassier, P., Bagdassarian, D., De Besse, N., Papillion, M., Baldalla, L., Porcheron, P., 1988. Ecdysteroids and activation of epidermal cells in the Locust Locusta migratoria. Journal of Insect Physiology, 34:7, 669-677.
• Clarke, B. S., Jewess, P.J., 1990. The The inhibition of chitin synthesis in Spodeptera littoralis larvae by flufenoxuron, teflubenzuron and diflubenzuron. Pesticide Science, 28: 377- 388
• Dorn, A., Redemacher, J.M. and Sehn, E., 1986. Effects of azadirachtin on the moulting cycle, endocrine system, and ovaries in last-instar larvae of the milkweed bug, Oncopeltus fasciatus. Journal of Insect Physiology, 32:3, 231- 238.
• Finney, D.J., 1971. Probit analysis (3 rd. Edn.) London: Cambridge University press.
• Garcia, E.S., Azamburja, P., Forster, H. and Rembold, H., 1984. Feeding and molt inhibition by azadirachtins A, B and 7- acethyl-azadirachtin A in Rhodnius prolixus nymphs. Zeitschrift für Naturforschung, 39:155-1158.
• Hegazy, G., Van De Veire, M. and Degheele, D., 1980. Effect of the chitin synthesis inhibitor diflubenzuron on the sixth and seventh instar of Gallaria mellonella L.: Importance of application time. Meded Fac Londbouvw. Rijksuniv. Gent, 45:453-463.
• Hegazy, G., De Cock, A., Auda, M. and Degheele, D., 1989. Diflubenzuron toxicity effect on the cuticle ultrastucture and chitin and protein content of Colarado potato beetle Leptinotursa decemlineata (Say) (Coleoptera:Chrysomelidae). Meded Fac Landbouw Rijks univ.Gent, 54:89-101.
• Hegazy, G. and Degheele, D., 1992. The effect of DFB on the ultrastructure of the Integument during moult events of Musca domestica. Parasitica, 48:3-14.
• Ker, R.F., 1978. The effects of diflubenzuron on the growth of insect cuticle. Pesticide Science, 9:259-265.
• Koul, O. and Isman M.O., 1991. Effect of azadirachtin on the dietary utilization and development of the varriegated cutworm Peridroma saucia. Journal of Insect Physiology, 37:591-598.
• Lee, S.A., Clarke, B.S., Jenner, D.W. and Williamson, F.A., 1990. Cytochemical demonstration of the effects of the acylureas flufenoxuron and diflubenzuron on the incorporation of chitin into insect cuticle. Pesticide Science, 28:367- 375.
• Manal, M.. Adel, F.S., 2000. Azadirachtin poten tiates the action of ecdysteroid agonist RH- 2485 in Epodeptera litteralis. Journal of insect physiology,46:267-274.
• Grisakova, M., Metspalu, L., Jogar, K., Hiiesaar, K., Kuusik, A. and Poldma, P., 2006. Effect of biopesticide Neem EC on the large White Butterfly, Pieris brassicae L.( Lepidoptera, Pieridae) Agronomy Research 4 (Special issue):181-186.
• Mitsui, T., Nobusava, C., Fukami, J., Colins, J. and Riddiford, L.M., 1980. Inhibition of chitin synthesis by diflubenzuron in Manduca larvae. Journal of Pesticide Science, 5: 335- 341.
• Mondal, K.A.M.S.H. and Parveen, S., 2000. Insect growth regulators and potential in the menagement of stored- product insect pests. Integrated pest management, 5:255- 295.
• Mordue (Luntz), A. J. and Blackwell, A., 1993. Azadirachtin: An update. Journal of insect physiology, 39:903-924.
• Mordue (Luntz), A.J., Nısbet, A.J., 2000. Azadirachtin from neem tree Azadirachta indica: Its action against insects. Anais da Sociedade Entomologica do Brasil, 29:4, 615- 632.
• Mordue (Luntz), A.J., 2004. Present consepts of the mode of action of azadirachtin from neem In Koul, O. S. (eds): Neem: today and in the new millennium. Kluser Academic publishers, London. 229-249.
• Osman, S.E., 1985. Effect of antimoulting agent “Dimilin” on the blood picture and cuticle formation in Spodoptera littoralis (Boisd) Larvae. Bulltein of the Entomological Society of Egypt, 14:37-46.
• Rahimin, N. and Soltani, N., 1999. Laboratory evaluation of Alsystin, a chitin synthesis inhibitor, against Culex pipiens pipiens L. (Dip, Culicidae): effect on development and cuticle secretion. Journal of Applied Entomology, 123:437-441.
• Ren, J.C., Ma, Y. and Chang, J.T., 1988. Microscopic observation on the histopatological changes of cuticle induced by DFB in two insect larvae. Acta Entomologica Sinica, 31: 366-370 (Chinese, with Engilish abstract).
• Reynolds, S.E., 1987. The cuticle, growth and moulting in insects: the essential background to the action acylurea insecticides. Pesticide Science, 20:131-146.
• Riba, M.J.M. and Sans, A., 2003. Influence of Azadirachtin on development of reproduction of Nezara viridula L. (Het., Pentomidae). Journal of Applied Entomology, 127: 37-41.
• Schmutterer, H., 1988. Potential of azadirachtin–containing pesticides for integrated pest control in developing and industralized countries. Journal of insect physiology, 34 :7, 713- 719.
• Soltani, N., Besson M. T. and Delchambre, J., 1984. Effect of diflubenzuron on the pupal adult development of Tenebrio molitor L. (Coleoptera,Tenebrionidae): Growth and development, cuticle secretion, epidermal cell density and DNA synthesis. Pesticide Biochemistry and Physiology, 21:256-264.
• Unsal, S., Ozparlak, H. and Aktumsek, A., 2004. Effect of Diflubenzuron on the Integument of Fifth Instar Gallaria mellonella L. larvae. Phytoparasitica, 32 :1, 43-51.
• Vinuela, E. and Budia, F., 1994. Ultrastructure of Ceratitis capitata Wiedemann larval integument and changes induced by the IGI cyromazine. Pesticide Biochemistry and Physiology, 48:191-201.
• Webb, R.E.M.A., Hinebaugh, R., Lindguist, K. and Jacobson, M., 1983. Evaluation of aquareous solution of neem seed extract against Liriomyza sativae and L. trifolii (Diptera, Agromyzidae). Journal Icon Entomology, 76:2, 357-362
• Yıldız, M., Gürkan, M.O., Turgut, C., Kaya, V., Ünal, G., 2005. Tarımsal savaşımda kullanılan pestisitlerin yol açtığı çevre sorunları. VI. Türkiye Ziraat Mühemdisliği Teknik Kongresi 3-7 Ocak, 2005. Ankara.
• Yin- Chang, W. Rong-Sheng, C., Chang-Kun, C. and Zi- Ping, Y., 1990. Studies on cuticular structures in two species of cutworms Acta Entomologica Sinica, 33:309-313 (Chinese, with English abstract).