ijfer

International Journal of Food Engineering Research

2149-5777

Istanbul Aydin University

PHENOTYPIC DETERMINATION OF ESBL- and AmpC- PRODUCING ENTEROBACTERIACEAE IN CHEESE SAMPLES

Özadam

Aylin

İstanbul Aydın University, Department of Food Safety Özpınar

Haydar

İstanbul Aydın University, Department of Food Engineering

06 01 2016

2 2 59 70

2015

International Journal of Food Engineering Research

The off-label over use of antibiotics results in development of antibiotic resistance in the bacteria. Beta-lactamase producing Enterobacteriaceae adversely affects the human health by leading to therapeutic failures against infections. Although microbiological criteria have been considered appropriate to the Food Codex, an inspection for antibiotic-resistant enterobacteria has not come into force yet. Therefore, the detection of foodborne beta-lactamases has gained significant importance for the human health. The objective of this study was to determine ESBLand AmpC- producing Enterobacteriaceae in cheese phenotypically. In this study, a total of 83 cheese samples was examined by performing pre-enrichment, enrichment on selective media, and oxidase test according to the Criteria by ISO/DIS21528-2 microbiologically. Based on the microbiological results, a total of 18 isolates, including Klebsiella pneumoniae 27.8% , Hafnia alvei 27.8% , Escherichia coli 22.2% , Klebsiella oxytoca 11.2% , Enterobacter cloacae 5.5% , and Citrobacter spp. 5.5% was identified by mass spectrometer. The phenotypic characterization of beta-lactamase type was conducted by disc diffusion, disc diffusion confirmation, and MIC determination according to the Guidelines of Clinical and Laboratory Standards Institute. The phenotypic results revealed that the most common beta-lactamase type was determined as ESBL in 9 isolates, followed by ESBL & AmpC in 4 isolates, and AmpC in 5 isolates, respectively. In conclusion, our study showed that ESBL- and Amp- type betalactamases were the most common phenotypes in Enterobacteriaceae from cheese. The cheese samples containing ESBL- and Amp- positive bacteria significantly presented a health risk for the consumers

Antibiotic resistance beta-lactamase cheese Enterobacteriaceae food safety public health

Van Boeckel TP, Brower C, Gilbert M, et al. (2015): Global trends in antimicrobial use in food animals. Proc Natl Acad Sci USA, 112, 5649- 5654.

Tekiner IH, Özpınar H (2016): Occurrence and characteristics of ESBL-producing Enterobacteriaceae from foods of animal origin. Braz J Microbiol, DOI: 10.1016/j.bjm.2015.11.034.

Falagas ME, Karageorgopoulos DE (2009): Extended-spectrum beta- lactamase-producing organisms. J Hosp Infect, 73, 345-354.

Shashwati N, Kiran T, Dhanvijay AG (2014): Study of extended spectrum β-lactamase producing Enterobacteriaceae and antibiotic coresistance in a tertiary care teaching hospital. J Nat Sci Biol Med, 5: 30–35.

Morosini MI, García-Castillo M, Coque TM, and et al (2006): Antibiotic coresistance in extended- spectrum-beta-lactamase-producing Enterobacteriaceae and in vitro activity of tigecycline. Antimicrob Agents Chemother, 50, 2695-2699.

Babic M, Hujer AM, Bonomo RA (2006): What's new in antibiotic resistance? Focus on beta- lactamases. Drug Resist Updat, 9:142-156.

Samaha-Kfoury JN, Araj GF (2003): Recent developments in beta lactamases and extended spectrum beta lactamases. BMJ, 327, 1209- 1213.

Zurfluh K, Hächler H, Nüesch- Inderbinen M, et al. (2013): Characteristics of extended-spectrum β-lactamase- and carbapenemase- producing Enterobacteriaceae Isolates from rivers and lakes in Switzerland. Appl Environ Microbiol, 79, 3021-3026.

McEwen SA, Fedorka-Cray PJ (2002): Antimicrobial use and resistance in animals. Clin Infect Dis, 34(Suppl 3), 93-106.

Lavilla S, Gonzalez-Lopez JJ, Miro E, et al. (2008): Dissemination of extended-spectrum beta –lactamase – producing bacteria: The food-borne outbreak lesson. J Antimicrobial Chem, 61, 1244-1251. pattern has contributed to their uncontrolled spread [2].

Van Boeckel TP, Brower C, Gilbert M, et al. (2015): Global trends in antimicrobial use in food animals. Proc Natl Acad Sci USA, 112, 5649- 5654.

Tekiner IH, Özpınar H (2016): Occurrence and characteristics of ESBL-producing Enterobacteriaceae from foods of animal origin. Braz J Microbiol, DOI: 10.1016/j.bjm.2015.11.034.

Falagas ME, Karageorgopoulos DE (2009): Extended-spectrum beta- lactamase-producing organisms. J Hosp Infect, 73, 345-354.

Babic M, Hujer AM, Bonomo RA (2006): What's new in antibiotic resistance? Focus on beta- lactamases. Drug Resist Updat, 9:142-156.

Samaha-Kfoury JN, Araj GF (2003): Recent developments in beta lactamases and extended spectrum beta lactamases. BMJ, 327, 1209- 1213.

McEwen SA, Fedorka-Cray PJ (2002): Antimicrobial use and resistance in animals. Clin Infect Dis, 34(Suppl 3), 93-106.

Lavilla S, Gonzalez-Lopez JJ, Miro E, et al. (2008): Dissemination of extended-spectrum beta –lactamase – producing bacteria: The food-borne outbreak lesson. J Antimicrobial Chem, 61, 1244-1251.

Apata DF (2009): Antibiotic resistance in poultry. Int J Poult Sci, 8, 404-408.

Dai L, Lu LM, Wu CM, and et al (2008): antimicrobial resistance among Escherichia coli isolates from chickens in China between 2001 and 2006. FEMS Microbiol Lett, 286, 178–183.

CLSI Clinical and Laboratory Standards Institute (2013): Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement, CLSI Document M100- S23, CLSI, Wayne PA.

Webb GF, D'Agata EMC, Magal P, et al (2002): A model of antibiotic- resistant bacterial epidemics in hospitals. Proc Natl Acad Sci, 99, 2293-2298.

EFSA (2011): Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β- lactamases in food and food- producing animals. EFSA Journal, 9, 2322-2417.

Neu HC (1992): The Crisis in Antibiotic Resistance. Science, 257, 1064-1073.

WHO (2015): Global Action plan on antimicrobial resistance. ISBN 9789241509763. Avenue Appia 20, 1211 Geneva, Switzerland.

Ata Z, Dinç G Yılbar A et al. (2015): Extended Spectrum beta- lactamase activity and multidrug resistance of Salmonella serovars isolated from chicken carcasses from different regions of Turkey. Ankara Ünv Vet Fak Derg, 62, 119-123.

O’Neill J (2014): Antimicrobial Resistance: Tackling a crisis for the health and wealth of nations. Review on Antimicrobial Resistance. (Available at http://amr-review.org). (Accessed December 2014).

Gündogan N, Avci E (2013): Prevalence and antibiotic resistance of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella species isolated from foods of animal origin in Turkey. Afr J Microbiol Res, 7, 4059-4064.

Kaya S, Çetin E, Arıkan S, et al. (2007): Tavuklarda izole edilen E coli, Klebsiella ve enterokoklarda antibiyotik duyarlılık durumları. SDÜ Tıp Fak Dergisi, 14, 24-27.

Alvarez-Fernandez E, Cancelo A, Diaz-Vega C, et al. (2013): Antimicrobial resistance in E. coli isolates from conventionally and organically reared poultry: a comparison of agar disc diffusion and Sensi Test Gram-negative methods. Food Control, 30, 227-234.

Stuart JC, van den Munckhof T, Voets G, et al. (2012): Comparison of ESBL contamination in organic and conventional retail chicken meat. Int J Food Microbiol, 154, 212-214.

Sudarwanto M, Akineden Ö, Odenthal S, et al. (2015): Extended- Spectrum β-Lactamase (ESBL)- Producing Klebsiella pneumoniae in Bulk Tank Milk from Dairy Farms in Indonesia. Foodborne Pathog Dis, 12, 585-590.

Overdevest I, Willemsen I, Rijnsburger M, et al. (2011): Extended-spectrum beta- lactamase genes of Escherichia coli in chicken meat and humans, the Netherlands. Emerg Infect Dis, 17, 1216-1222.

Van den Bogaard AE, Stobberingh EE (2000): Epidemiology of resistance to antibiotics. Links between animals and humans. Int J Antimicrob Agents, 14, 327-335.

Van den Bogaard AE (2001): Human health aspects of antibiotic use in food animals: a review. Tijdschr Diergeneeskd, 126, 590-595.

Lopez-Cerrero L, Egea P, Torres E, et al. (2012): Increased raw poultry meat colonization by extended spectrum beta-lactamase- producing Escherichia coli in the South of Spain. Int J Food Microbiol, 159, 69- 73.

Smet A, Martel A, Persoons D, et al. (2009): Broad-spectrum β- lactamases among Enterobacteriaceae of animal origin: molecular aspects, mobility and impact on public health. FEMS Microbiology Reviews, 34, 295-316.

Schwaiger K, Huther S, Hölzel C, et al. (2012): Prevalence of antibiotic-resistant enterobacteriaceae isolated from chicken and pork meat purchased at the slaughterhouse and at retail in Bavaria, Germany. International Journal of Food Microbiology, 154, 206–211.